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序号 专利名 申请号 申请日 公开(公告)号 公开(公告)日 发明人
141 Transformation vector for developing plant endotoxin and itsapplication JP779986 1986-01-17 JPS62111689A 1987-05-22 GREVE HENRI MARCEL JOZEF DE; SALGADO MARIA BENITA LEONOR FE; MONTAGU MARC CHARLES ERNEST VA; VAECK MARK ALBERT; ZABEAU MARCUS FLORENT OSCAR; LEEMANS JAN JOZEF AUGUST; HOFTE HERMANUS FRANSISCUS PAUL
(1) Chimeric gene capable of being expressed in differentiated plant cells comprising (a) DNA fragment comprising a promoter region derived from a gene naturally expressed in a plant cell; and (b) a DNA fragment (s) coding for a polypeptide toxin produced by Bacillus thuringiensis or having sequence homology to it is new. (2) Hybrid plasmid rector comprising (a) a DNA fragment(s) delineating a DNA fragment to be integrated into a plant cell genome; and (b) a chimeric gene(s) as defined above is new. (3) Intermediate plasmid vector contg. a chimeric gene(s) as defined above is new. (4) Plant, plant seed or plant tissue including in its cells genome and expressing a chimeric gene as defined above (the seed or tissue germinating into the plant expressing the gene) is new. (5) Plasmids harboured within cell cultures deposited as DSM 3127-3130 and ATCC 53385-53390 and 53392-53402 are new.
142 식물 엽록체에서의 대장균 장독소 B서브유닛의 고발현 방법 KR1020030021346 2003-04-04 KR1020040087094A 2004-10-13 양문식; 강태진
PURPOSE: A high expression method of the B subunit of E. coli heat-labile enterotoxin in the chloroplast of plants is provided, thereby easily and efficiently forming immune response of the animal by introducing plants expressing animal disease inducing antigen into animals. CONSTITUTION: An antigen expression cassette for transforming the chloroplast of plants comprises (a) chloroplast rRNA operon promoter(Prrn); (b) ribosome binding nucleotide sequence; (c) antibiotic resistance gene; (d) polynucleotide containing the nucleotide sequence encoding an animal disease inducing antigen; and (e) 3'-untranslated region of chloroplast psbA gene, wherein the animal disease inducing antigen is B subunit of E. coli enterotoxin. A vector pMY-CH-LTB comprises the antigen expression cassette. A transgenic plant containing the antigen expression cassette in the chromosomal genome by homologous recombination, wherein the plant is animal edible plant; and the plant is Nicotiana tabacum/pMY-CH-LTB(KCTC 10447BP). The high expression method of the B subunit of E. coli heat-labile enterotoxin in the chloroplast of plants comprises inserting the antigen expression cassette into the chromosomal genome of a plant and expressing the animal disease inducing antigen from the plant chromosome.
143 Antibody production and detecting method for detection of gonyautoxin from phytoplankton KR20100119317 2010-11-29 KR20120057808A 2012-06-07 CHANG MAN; LEE JAE HAC; LEE TAEK KYUN
PURPOSE: A biosensor for detecting gonyautoxin secreted by phytoplankton is provided to early detect toxin using a precise analyzer and to diagnose other harmful toxin. CONSTITUTION: A biosensor for detecting phytoplankton-derived gonyautoxin contains anti-GTX antiserum isolated from a mouse which is immunized with a conjugate of GTX and proteins. The proteins are ovalbumin(OVA) or bovine serum albumin(BSA). A method for detecting gonyautoxin comprises: a step of preparing the conjugate; a step of immunizing by injecting the conjugate to the mouse; and a step of measuring chromogenesity by antigen-antibody reaction of antiserum and gonyautoxin.
144 적용 또는 유도된 옥신에 의한 식물 병원균 및 해충 억제 KR1020067003511 2004-08-18 KR1020070024448A 2007-03-02 스톨러제리에이치
The present invention is directed to methods for inhibiting the growth of disease organisms, particularly fungi and bacteria, on plant tissues. The present invention is also directed to methods for inhibiting the infestation of plants by insects and larva, particularly sucking and chewing insects. These methods are achieved by applying an auxin or a plant growth regulator (PGR) which will effect the level of auxin in the plant tissue to the seeds or tubers of the plant prior to planting or to the roots, foliage, flowers or fruit of the plant after planting. The auxin or PGR is applied in an amount effective to inhibit growth of the disease organisms or insects, but in an amount insufficient to negatively effect growth of the plant tissues. The auxin may be applied as a natural auxin, synthetic auxin, auxin metabolite, auxin precursor, auxin derivative or a mixture thereof. The presently preferred auxin is indole-3-acetic acid (IAA). The auxin or PGR may be applied to the seeds, tubers or plant tissues. Seeds or tubers may be sprayed with or immersed in an aqueous solution containing the auxin or PGR. Conventional spraying and drip irrigation systems may be used to apply an aqueous solution containing an auxin or PGR to plant tissues. The auxin or PGR may also be applied to the plant tissues as a powder or may be encapsulated within a biologically compatible material to provide slow release to the roots of the plant. The plant tissues may be dusted with a powder, including the auxin or PGR. The encapsulated auxin may be placed in the root zone for uptake of the auxin or PGR by the roots. ® KIPO & WIPO 2007
145 장내 독소 제거 기능을 갖는 식물 복발효 효소액 및 이를사용한 기능성 음료 KR1020040053494 2004-07-09 KR100512323B1 2005-09-02 김양문; 배미정; 김솔
본 발명은 장내 이상 발효에 의한 독소(가스)를 제거하는 효과를 갖는 식물 복발효 효소액에 관한 것이다. 본 발명은 본 발명은 3∼6 중량%의 개감수, 3∼6 중량%의 나팔꽃, 3∼6 중량%의 맥문동, 3∼6 중량%의 꽃다지, 3∼6 중량%의 다닥냉이, 3∼6 중량%의 지치, 5∼10 중량%의 민들레, 3∼6 중량%의 차즈기, 3∼6 중량%의 참당귀, 3∼6 중량%의 탱자나무, 5∼10 중량%의 대추나무, 5∼10 중량%의 뽕나무, 3∼6 중량%의 살구나무, 3∼6 중량%의 자두나무, 5∼10 중량%의 잣나무, 3∼6 중량%의 금불초 및 5∼10 중량%의 녹나무로 이루어진 약재를 분쇄하여 혼합하는 단계와; 상기 혼합물을 전고형분 중량대비 2∼3배의 물에 넣고 35∼48℃의 중온에서 유효성분을 추출하는 단계와; 상기 추출된 추출액 200중량부에 천연과즙 50∼60 중량부와 곡물분말 40∼50 중량부를 첨가하여 균일하게 혼합한 다음, 포도당 40∼60 중량부와 설탕 40∼60 중량부를 첨가하고, 10∼15℃에서 2∼3개월간 pH 3.5∼4.5로 저온 발효하는 단계와; 상기 저온 발효된 발효액을 여과한 여액에 젖산균을 첨가하여 15∼35℃에서 3∼5일간 pH 3.5∼4.5로 젖산 발효하는 단계; 및 상기 젖산발효된 발효액을 여과하여 남은 여액을 수득한 장내 독소 제거 기능을 갖는 식물 복발효 효소액을 제공한다. 본 발명은 장기간 복용해도 부작용이 없고 장기능을 활성화하여 변비 개선은 물론 장내 이상 발효를 예방하고, 장내 독소 제거 효능이 우수한 식물의 복발효 효소액을 제공한다.
146 적용 또는 유도된 옥신에 의한 식물 병원균 및 해충 억제 KR1020067003511 2004-08-18 KR101120972B1 2012-03-08 스톨러제리에이치
본발명은식물조직상의질병생물, 특히곰팡이및 박테리아의성장을억제시키기위한방법에관한것이다. 본발명은또한곤충및 유충, 특히흡입곤충및 저작곤충에의한식물의감염을억제시키기위한방법이다. 이러한방법은식물조직내의옥신수준에영향을미치는옥신또는식물성장조절물질 (PGR) 을식재전에식물의종자또는덩이줄기에, 또는식재후에식물의뿌리, 군엽, 꽃또는열매에적용하여달성된다. 옥신또는 PGR 은질병생물또는곤충의성장을억제하기에효과적이지만, 식물조직에부정적인영향을미치기에는불충분한양으로적용된다. 옥신은천연옥신, 합성옥신, 옥신대사물질, 옥신전구체, 옥신유도체또는그의혼합물로적용될수 있다. 현재바람직한옥신은인돌-3-아세트산 (IAA) 이다. 옥신또는 PGR 은종자, 덩이줄기또는식물조직에적용될수 있다. 종자또는덩이줄기는옥신또는 PGR 을함유하는수성용액으로분무되거나그에담그어질수 있다. 통상적인분무및 적하관개시스템이, 옥신또는 PGR 을함유하는수성용액을식물조직에적용하기위해사용될수 있다. 또한, 옥신또는 PGR 은식물조직에분말로적용되거나생물학적으로친화적인물질내에캡슐화되어식물의뿌리로서서히방출될수 있다. 식물조직은옥신또는 PGR 을함유하는분말로살포될수 있다. 캡슐화된옥신은뿌리지대에놓여, 뿌리에의해옥신또는 PGR 이흡수될수 있다.
147 불투명 심백배유 발현과 웅성불임을 유발시키는다면발현성을 갖는 식물체의 UGPase1 유전자 KR1020070137046 2007-12-26 KR1020090069397A 2009-07-01 고희종; 우미옥; 함태호; 강문수; 추상호; 최민선
A pleiotropic UGPase1 gene which causes chalky endosperm and male-sterility by substituting mono nucleotide in the UGPase1 gene is provided. A plant forms chalky endosperm by mutating a rice-derived UGPase 1 gene which comprises base sequence of the sequence number 1(SEQ ID NO:1). The amylase content, gel consistency or magnesium/potassium ratio of chalky endosperm is reduced comparing a normal endosperm. The protein content, lipid content, magnesium and calcium content or fatty acid total content is increased comparing the normal endosperm. The starch structure of chalky endosperm has more straight chain amylase and an amylopectin having more branches. The UGPase1 gene has pleiotropic property and causes chalky endosperm and male-sterility.
148 알콕시메틸리덴 에피포도필로톡신 배당체 KR1019900018740 1990-11-19 KR100068220B1 1993-12-02 다께시오오누마; 다까유끼나이또; 히데오가메이
149 액분사력에 의한 식물해충 구제방법및 그 장치 KR1020030081800 2003-11-18 KR100549561B1 2006-02-08 야노하라요시타미
불필요한 설비의 대형화를 피하는 것이 가능하고, 키가 크고 적은 어느 식물에 대해서도 해충구제를 행하는 것이 가능한 해충구제 방법을 제공한다. 해충이 떨어지는 액체의 액면상에 해충이 부착한 식물을 지지하고, 상기 식물에 대하여 액체를 다방향으로부터 분사하며, 그 분사력에 의하여 식물에 부착한 해충을 불어서 떨어트려 상기 해충이 떨어지는 액체로 흘러 내리도록 하는 액분사력에 의한 식물해충 구제방법이다. 해충이 떨어지는 액체, 해충 부착 식물, 액분사력, 식물해충 구제방법, 식물해충 구제장치
150 피토알렉신 생산 방법 및 그에 의해 생산된 피토알렉신 KR1020110074700 2011-07-27 KR101841620B1 2018-03-26 전희영; 서대방; 조시영; 이상준
본발명은종자를발아시키는발아공정; 및종자를발효시키는발효공정을포함하며, 상기종자는콩을포함하지않는피토알렉신생산방법을개시한다. 또한본 발명은피토알렉신생산방법으로서, 종자를발아시키는발아공정; 및종자를발효시키는발효공정을포함하며, 상기피토알렉신은쿠메스트롤을포함하지않는피토알렉신생산방법을개시한다.
151 METHODS FOR TREATING A PLANT EXPOSED TO A PHYTOTOXICANT PCT/US2005/007172 2005-03-02 WO2005087691A1 2005-09-22 YAMASHITA, Thomas, T.

Methods of treating a plant exposed to a phytotoxicant are provided. Embodiments of the subject methods include identifying a plant exposed to a phytotoxicant and applying an assimilable carbon-skeleton energy component-comprising composition to the identified plant. Embodiments of the subject compositions may include one or more of a macronutrient component, micronutrient component, vitamin/cofactor component, complexing agent and microbe. Kits for use in practicing the subject invention are also provided. The subject methods find use in a variety of different applications in which a plant is phytotoxic or at least in danger of becoming phytotoxic due to exposure or potential exposure to a phytotoxicant.

152 USE OF PHYCOTOXINS IN VETERINARY APPLICATIONS PCT/US2006017449 2006-05-05 WO2006119499A2 2006-11-09 WILSON NESTOR ANTONIO LAGOS
Pharmaceutical compositions comprising tricyclic 3,4-propinoperhydropurines and uses thereof which for providing a local anesthetic, muscle relaxant, facilitating wound healing, and immobilization to an animal are provided. Also provided are a variety of methods of treating animals using the compositions of the invention.
153 ENHANCEMENT OF PRODUCTION OF CAMPTOTHECINS FROM PLANTS PCT/US0029344 2000-10-25 WO0130753A9 2002-05-16 LI SHIYOU
The present invention provides a system for increasing the production of indole and quinoline alkaloids, particularly camptothecins and related compounds, in plants, based upon management of plant hormones. This invention includes a system and a method for accelerating the growth of young tissues, inducing the production of camptothecins-bearing glandular trichomes, maximizing the harvest of glandular trichomes, and preserving and treating the glandular trichomes for maximum camptothecins yield. The present invention is useful for any plant matters containing alkaloids, regardless of the plant matters' genetic origin, natural or cultivated variety, and natural or cultivated growing conditions.
154 ORALLY IMMUNOGENIC BACTERIAL ENTEROTOXINS EXPRESSED IN TRANSGENIC PLANTS PCT/US9930747 1999-12-22 WO0037609A2 2000-06-29 MASON HUGH S; ARNTZEN CHARLES J
The invention provides mutant Escherichia coli heat labile (LT) and Vibrio cholerae toxin (CT) polypeptides and the polynucleotides that encode them. The mutant LT and CT polypeptides can be readily produced in plants and can be used to treat or prevent diseases caused by E. coli and V. cholera. The polypeptides are also useful as adjuvants.
155 PLANT-OPTIMIZED GENES ENCODING PESTICIDAL TOXINS PCT/US9823457 1998-11-04 WO9924581A3 1999-09-30 CARDINEAU GUY A; STELMAN STEVEN J; NARVA KENNETH E
The subject invention concerns materials and methods useful in the control of pests and, particularly, plant pests. More specifically, the subject invention provides plant-optimized, polynucleotide sequences that encode pesticidal toxins (full-length and truncated). Truncated polynucleotide sequences can be used to produce truncated toxins or for the production of fusion (or chimeric) genes and proteins. The polynucleotide sequences of the subject invention have certain modifications, compared to wild-type sequences, that make them particularly well-suited for optimized expression in plants. Using techniques known to those skilled in the art, the polynucleotide sequences described herein can be used to transfer plants in order to confer pest resistance upon said plants. The subject invention further provides plant-optimized, polynucleotide sequences that encode C-terminal, protoxin portions that can be used with genes encoding truncated, core toxins to produce full-length toxins. In addition, the subject invention provides preferred amino acid sequences that are encoded by the plant-optimized polynucleotide sequences disclosed herein.
156 METHOD OF PROTECTING SEEDS TREATED WITH A PHYTOTOXIC AGENT PCT/US0336178 2003-12-01 WO2004049778A9 2005-09-22 ASRAR JAWED; BEKKER VLADIMIR O; DING YIWEI
A method of improving germination rate in pesticide-treated plant seeds involves forming a pesticide-free polymer coating on a plant seed before treating the seed with a pesticide, where the type of polymer and the coating thickness are designed to block phytotoxic contact of the pesticide with the seed while allowing sufficient transfer of oxygen to maintain the seed's viability and sufficient transfer of moisture under environmental conditions normally encountered by the seed after planting to enable its germination; and then treating the coated plant seed with a pesticide. Seeds that have been treated by this method, and plants that are grown from the treated seeds are also described.
157 METHOD OF PROTECTING SEEDS TREATED WITH A PHYTOTOXIC AGENT PCT/US2003/036178 2003-12-01 WO2004049778A1 2004-06-17 ASRAR, Jawed; BEKKER, Vladimir, O.; DING, Yiwei

A method of improving germination rate in pesticide-treated plant seeds involves forming a pesticide-free polymer coating on a plant seed before treating the seed with a pesticide, where the type of polymer and the coating thickness are designed to block phytotoxic contact of the pesticide with the seed while allowing sufficient transfer of oxygen to maintain the seed's viability and sufficient transfer of moisture under environmental conditions normally encountered by the seed after planting to enable its germination; and then treating the coated plant seed with a pesticide. Seeds that have been treated by this method, and plants that are grown from the treated seeds are also described.

158 PLANT-OPTIMIZED GENES ENCODING PESTICIDAL CYTC-TYPE TOXINS PCT/US1999/024648 1999-10-21 WO00024905A1 2000-05-04
The subject invention concerns materials and methods useful in the control of pests and, particularly, plant pests. More specifically, the subject invention provides new, plant-optimized polynucleotide sequences that encode pesticidal toxins. The polynucleotide sequences of the subject invention have certain modifications, compared to wild-type sequences, that make them particularly well-suited for optimized expression in plants. Using the polynucleotide sequences described herein, the transformation of plants can be accomplished, using techniques known to those skilled in the art, in order to confer pest resistance upon said plants. In preferred embodiments, the subject invention provides plant-opimized polynucleotide sequences which encode toxin proteins that can be referred to as cytC-type toxins. A preferred toxin encoded by a plant-optimized gene is also disclosed.
159 PLANT-OPTIMIZED GENES ENCODING PESTICIDAL TOXINS PCT/US1998/023457 1998-11-04 WO99024581A2 1999-05-20
The subject invention concerns materials and methods useful in the control of pests and, particularly, plant pests. More specifically, the subject invention provides plant-optimized, polynucleotide sequences that encode pesticidal toxins (full-length and truncated). Truncated polynucleotide sequences can be used to produce truncated toxins or for the production of fusion (or chimeric) genes and proteins. The polynucleotide sequences of the subject invention have certain modifications, compared to wild-type sequences, that make them particularly well-suited for optimized expression in plants. Using techniques known to those skilled in the art, the polynucleotide sequences described herein can be used to transfer plants in order to confer pest resistance upon said plants. The subject invention further provides plant-optimized, polynucleotide sequences that encode C-terminal, protoxin portions that can be used with genes encoding truncated, core toxins to produce full-length toxins. In addition, the subject invention provides preferred amino acid sequences that are encoded by the plant-optimized polynucleotide sequences disclosed herein.
160 PLANTS TRANSFORMED TO PRODUCE BACILLUS THURINGIENSIS INSECTICIDAL TOXINS PCT/EP1990000244 1990-02-14 WO1990009445A1 1990-08-23 PLANT GENETIC SYSTEMS, N.V.
Two new $i(Bacillus thuringiensis) strains, which are deposited at the DSM under accession nos. 5131 and 5132, produce crystal proteins during sporulation that are toxic to Coleoptera. The crystal proteins contain 74 kDa and 129 kDa protoxins, respectively, which can yield 68 and 66 kDa toxins, respectively, as trypsin-digestion products. A plant, the genome of which is transformed with a DNA sequence that comes from either one of the strains and that codes for its respective toxin, is resistant to Coleoptera. Each strain, itself, or its crystals, crystal proteins, protoxin or toxin can be used as the active ingredient in an insecticidal composition for combatting Coleoptera.
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