Method of preparing high yields of double-stranded ribonucleic acid专利检索-宿主植物生物防治专利检索查询-专利查询网

Method of preparing high yields of double-stranded ribonucleic acid

阅读：122发布：2023-09-21

专利汇可以提供Method of preparing high yields of double-stranded ribonucleic acid专利检索，专利查询，专利分析的服务。并且An unusual bacteriophage phi 6, ATCC No. 21781-B, containing a lipid coat and double-stranded ribonucleic acid has been isolated for the plant pathogenic bacterium Pseudomonas phaseolicola ATCC No. 21781. High yields of double-stranded ribonucleic acid may be obtained rapidly and expediently by cultivating a host bacterium for the bacteriophage, infecting the host bacteria with the bacteriophage to provide a lysate and recovering double-stranded ribonucleic acid from the separated bacteriophage. The intact or whole bacteriophage, the bacteriophage with its lipid coat removed, and the isolated nucleic acid have all been found to be effective interferon inducers.，下面是Method of preparing high yields of double-stranded ribonucleic acid专利的具体信息内容。

权利要求

2. The method of claim 1 wherein the double-stranded-containing bacteriophage is phage phi 6 having the ATCC reference No. 21781-B.

3. The method of claim 2 wherein the host bacteria are selected from strains of Pseudomonas phaseolicola, Pseudomonas glycinea and Pseudomonas syringae.

4. The method of claim 3 wherein the host bacteria are Pseudomonas phaseolicola, having ATCC reference No. 21781.

5. The method of claim 3 wherein the phage phi 6 is added when the Pseudomonas phaseolicola count is in the range of about 2 -10 X 108 cells per ml of nutrient medium.

6. The method of claim 2 wherein the recovery of said double-stranded ribonucleic acid comprises adding a deproteinizing solvent to an aqueous dispersion of said separated phage phi 6 to extract protein, centrifuging the mixture and recovering the resulting aqueous phase containing double-stranded ribonucleic acid.

7. The method of claim 6 wherein prior to said phenol extraction liquid is removed from said phage phi 6 by extraction with a lipid solvent or by treatment with lipid-removing enzymes.

8. The process of claim 6 wherein the deproteinizing solvent is phenol or phenol sodium dodecyl sulfate.

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Method of preparing high yields of double-stranded ribonucleic acid

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