| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 1 | 用于显微技术的方法与装置 | CN202110910336.1 | 2016-03-15 | CN113514945B | 2024-04-09 | 爱德华多·沃马; 西蒙·乔纳森·思朋斯; 塞缪尔·罗丝·加兰·蓝阳; 本尼迪克特·约翰·斯图尔特-斯蒂尔 |
| 本发明提供利用通过在延时测量间隔内同时地捕获生物样本的亮视野图像与暗视野图像的显微技术来评估生物样本的发育活力。 | ||||||
| 2 | 用于显微技术的方法与装置 | CN202110910336.1 | 2016-03-15 | CN113514945A | 2021-10-19 | 爱德华多·沃马; 西蒙·乔纳森·思朋斯; 塞缪尔·罗丝·加兰·蓝阳; 本尼迪克特·约翰·斯图尔特-斯蒂尔 |
| 本发明提供利用通过在延时测量间隔内同时地捕获生物样本的亮视野图像与暗视野图像的显微技术来评估生物样本的发育活力。 | ||||||
| 3 | 用于显微技术的方法与装置 | CN201680027834.9 | 2016-03-15 | CN107533217A | 2018-01-02 | 爱德华多·沃马; 西蒙·乔纳森·思朋斯; 塞缪尔·罗丝·加兰·蓝阳; 本尼迪克特·约翰·斯图尔特-斯蒂尔 |
| 本发明提供利用通过在延时测量间隔内同时地捕获生物样本的亮视野图像与暗视野图像的显微技术来评估生物样本的发育活力。 | ||||||
| 4 | 一种改进苗帘暗处理技术的简便高效海带育苗方法 | CN202010545612.4 | 2020-06-16 | CN111543305A | 2020-08-18 | 曲善村; 王伟伟; 盛宝利; 程朝霞; 王青岩; 史良; 赵楠; 孙娟; 田萍萍; 李晓捷 |
| 本发明公开了一种改进苗帘暗处理技术的简便高效海带育苗方法，育苗附着基由北方传统红棕绳更换为维尼纶绳，孢子采集阶段控制胚孢子在10-15个/160倍显微镜视野的低密度水平即可；采孢子完成后直接将苗帘多层叠加置于黑暗环境中，流水、低温保存，然后单层平铺育苗池中正常培育。本发明的优点在于：本发明的育苗方法适用于规模化海带苗种生产，不仅能获得高质量苗种，还简化了苗帘暗处理技术操作，压缩了海带前期育苗生产阶段占用生产车间的容量和生产投入，缩短了正常育苗时间，大幅降低了生产成本。 | ||||||
| 5 | 一种阿贝聚光镜挡光片托架 | CN202120754937.3 | 2021-04-13 | CN214335357U | 2021-10-01 | 刘书宝 |
| 一种新型阿贝聚光镜挡光片托架。特别涉及一种阿贝聚光镜挡光片托架。实用新型为克服现有技术存在当聚光镜挡光片托架安装在显微镜上时，不能实现明暗视野转换，进而不能更好地显示显微镜图像上的清晰度的问题。一种阿贝聚光镜挡光片托架，它包括托架和聚光镜挡光托架手动明暗场转换旋钮；托架上分别加工有明场光源出光口和暗场光源出光口，明场光源出光口的圆孔直径小于暗场光源出光口的圆孔直径，托架靠近暗场光源出光口处一侧端面上安装有聚光镜挡光托架手动明暗场转换旋钮。用于托架装置领域。 | ||||||
| 6 | 微纳米粒子计数装置 | CN201120430159.9 | 2011-11-03 | CN202330239U | 2012-07-11 | 孙永军 |
| 本实用新型是关于一种微纳米粒子计数装置，包括一玻璃片，所述玻璃片上有一平台，平台上面刻有方格网，方格网的网线上涂敷有涂层，所述涂层在暗视野下可发光。本实用新型与现有技术相比具有明显的优点和有益效果，在普通光学显微镜和聚光器构造的暗视野背景下，利用微纳米粒子计数器，计量出体积已知的溶液中微纳米粒子的个数，从而快速准确计算出微纳米粒子的浓度。 | ||||||
| 7 | 相差聚光镜 | CN200920030970.0 | 2009-07-31 | CN201444212U | 2010-04-28 | 谢晓鸿; 张宝运; 孙堂明 |
| 本实用新型提供一种相差聚光镜，属于显微镜配件技术领域，其结构包括底座、非球面透镜、相差环转盘、转轴、明场、暗场和相衬，相差环转盘通过转轴固定在底座上，在相差环转盘的周边分布有明场、暗场和相衬，非球面透镜固定在相差环转盘上，相差环转盘上的相衬有两个，且其中一个相衬的金属环位于相衬视野的中心，颜色为浅绿色；另一个相衬的金属环在相衬视野上呈偏中心设置，颜色为浅灰色。本实用新型的相差聚光镜与现有技术相比，在光学显微镜的基础上，将聚光器上的相差环转盘透光金属片孔径光阑进行光路装置改进，将原有的金属片孔径光阑加入专用多组滤色片进行光路色彩过滤，改变成单一光线，形成单一波长，使透光率更高，分辨率更强。 | ||||||
| 8 | 一种弱衍射特征晶体相的表征方法 | CN202311689827.3 | 2023-12-07 | CN117686534A | 2024-03-12 | 汪姚岑; 曹崇德 |
| 一种弱衍射特征晶体相的表征方法，步骤一：观察材料明场像，确定基本晶体分布状况与尺寸；步骤二：选择显微视野中的区域做多晶电子衍射，寻找至少一个符合目标相特征衍射的斑点，但物相结构的确定通常需要寻找多个特征单晶衍射斑或多个特征多晶衍射环，单个特征斑点通常不足以证明目标相存在；步骤三：在衍射斑点中选择符合目标相特征衍射的斑点，并以此为基准对衍射采样区域取暗场像；步骤四：初步筛选暗场像中发亮的若干晶体为疑似目标晶体，对其进行单晶衍射或高分辨拍照，并根据衍射图样或高分辨照片的晶面间距和空间频率特征确定目标有序晶体。将需要精细观测的疑似目标晶体数量从数千缩小到十余个的程度，由此可大幅减少需要分析观察的晶体数量；可利用高分辨成像+频域转换(FFT)技术对极小的观测区域进行结构衍射模拟分析，避免了对纳米尺度晶体观察需要纳米束斑的特殊要求。 | ||||||
| 9 | 一种便携式显微镜 | CN201220115862.5 | 2012-03-26 | CN202472111U | 2012-10-03 | 王文汇 |
| 本实用新型涉及显微镜，属于实验器具技术领域。一种便携式显微镜，包括底座，底座前面设置有电池盖，电池盖内放置有电池，电池盖一侧设有开关，底座的上面安装有压片夹，底座中部设有灯罩，灯罩内嵌有灯泡，底座的侧端通过转轴与实心轴连接，实心轴与镜筒之间通过活动支架连接，镜筒的两端分别设有目镜和物镜，镜筒为可伸缩结构。本实用新型的便携式显微镜，体积小，重量轻，便于携带，适用场合广泛，镜筒采用可伸缩结构进行调距，从而可以使放大倍数更大，光源采用的是照明灯泡，这就避免了因天气原因而导致的视野阴暗的缺陷，并且镜筒与实心轴之间采用的是活动支架连接，这样就方便了折叠，利于携带。 | ||||||
| 10 | 一种基于微流控芯片的多功能集成分析多孔细胞培养芯片 | CN201420543019.6 | 2014-09-22 | CN204198745U | 2015-03-11 | 秦建华; 许慧; 李中玉 |
| 一种基于微流控芯片的多功能集成分析多孔细胞培养芯片属细胞培养分析技术领域。该细胞培养芯片主要由细胞培养池、小皿组成；细胞培养池位于小皿内底部，与小皿可逆封接而成；细胞培养池上有2～6个孔室。所述细胞培养池的材料为可透光透气的PDMS聚合物，所述孔室由打孔器制出。所述细胞培养池为厚度为1mm～5mm。本实用新型细胞培养芯片具操作简便，成本低廉，试剂及细胞消耗量低，显微观察视野无明显的明暗影响，加液换液时细胞不易被冲起，细胞接种时密度更均一等优点，能够实现特定的实验目的，在细胞培养与分析评价方面有广泛的应用价值。 | ||||||
| 11 | 암시야 현미경 기술과 ｚ－ｄｅｐｔｈ 의존 공초점 표면증강 라만 산란을 이용한 단세포내 단백질 코팅된 금속 나노파티클의 탐지방법 | KR1020100108343 | 2010-11-02 | KR1020120046603A | 2012-05-10 | 주상우; 이소영; 조근창; 박진호 |
| PURPOSE: A method for detecting a protein-coated metal nanoparticle inside a single cell using a z-depth dependent confocal surface-enhanced Raman scattering and a dark-field microscopy technique informs absorption inside a cell of a metal nano particle within an A549 cell and behavioral characteristic thereof by using a super spectral imaging technique and a SERS(Surface Enhanced Raman Scattering) combined with a DFM(Dark-Field Microscopy). CONSTITUTION: A method for detecting a protein-coated metal nanoparticle inside a single cell is as follows. A protein-coated metal nanoparticle is manufactured. The nanoparticle is absorbed inside a cell and detects the absorbed nanoparticle by using a dark-field microscope technique and a z-depth dependent confocal surface-enhanced Raman scattering. | ||||||
| 12 | SERS 센서용 기판의 평가방법 | PCT/KR2017/004474 | 2017-04-27 | WO2017188744A1 | 2017-11-02 | 권혁상 |
본 발명은 a) 암시야 현미경을 통해 SERS 센서용 기판 상에 위치한 나노입자의 색상을 측정하는 단계; b) 측정된 색상을 나노입자간 거리로 환산하는 단계; c) SERS 센서용 기판의 라만 신호강도를 획득하는 단계; d) 환산된 거리와 동일한 거리를 가지는 나노입자를 포함하는 표준 SERS 센서용 기판의 표준 라만 신호강도를 획득하는 단계; 및 e) 라만 신호강도와 표준 라만 신호강도를 비교하는 단계;를 포함하는 SERS 센서용 기판의 평가방법에 관한 것이다. |
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| 13 | NOVEL GREEN AND ORANGE FLUORESCENT LABELS AND THEIR USES | PCT/US0330167 | 2003-09-23 | WO2004027388A3 | 2004-06-10 | BHATT RAM; CONRAD MICHAEL J; BENCHEIKH AZZOUZ; XIONG YIFENG |
| The present invention provides novel fluorescent compounds and covalent attachment chemistries which facilitate the use of these compounds as labels for ultrasensitive and quantitative fluorescent detection of low levels of biomolecules. In a preferred embodiment, the fluorescent labels of this invention are novel derivatives of the hydroxy-pyrene trisulphonic and disulphonic acids which may be used in any assay in which radioisotopes, colored dyes or other fluorescent molecules are currently used. Thus, for example, any assay using labeled antibodies, proteins, oligonucleotides or lipids, including fluorescent cell sorting, fluorescence microscopy (including dark-field microscopy), fluorescence polarization assays, ligand, receptor binding assays, receptor activation assays and diagnostic assays can benefit from use of the compounds disclosed herein. | ||||||
| 14 | 顕微鏡の照明装置 | JP2009001618 | 2009-03-18 | JP3150912U | 2009-06-04 | 耀昌 李; 佩▲ゆ▼ ▲黄▼ |
| 【課題】大部分の入射光源は浪費されず、イメージのコントラストを増加し、また、レーザー光を直接光源とすることができ、拡張なしで、イメージの解像力を向上させる顕微鏡の照明装置を提供する。【解決手段】顕微鏡の照明装置は、一対の凸面錐体鏡123aと凹面錐体鏡123bからなる一組の錐体鏡を利用して、暗視野顕微鏡に必要な環形光を生成し、両錐体鏡の同軸軌道に沿って凸面錐体鏡123aを滑動させて、環形光の大きさを連続で変化させる。この他、更に、明視野顕微鏡の照明を提供する。直接、凸面錐体鏡123aと連接する45度の柱状明視野顕微鏡を利用して、暗視野顕微鏡と同一光源、或いは、異なる光源が生成する光を受けたり、発射したりする。よって、デュアル照明功能を有し、現行の顕微鏡に応用できる。【選択図】図3 | ||||||
| 15 | NOVEL GREEN AND ORANGE FLUORESCENT LABELS AND THEIR USES | PCT/US2003/030167 | 2003-09-23 | WO2004027388A2 | 2004-04-01 | BHATT, Ram; CONRAD, Michael, J.; BENCHEIKH, Azzouz; XIONG Yifeng |
The present invention provides novel fluorescent compounds and covalent attachment chemistries which facilitate the use of these compounds as labels for ultrasensitive and quantitative fluorescent detection of low levels of biomolecules. In a preferred embodiment, the fluorescent labels of this invention are novel derivatives of the hydroxy-pyrene trisulphonic and disulphonic acids which may be used in any assay in which radioisotopes, colored dyes or other fluorescent molecules are currently used. Thus, for example, any assay using labeled antibodies, proteins, oligonucleotides or lipids, including fluorescent cell sorting, fluorescence microscopy (including dark-field microscopy), fluorescence polarization assays, ligand, receptor binding assays, receptor activation assays and diagnostic assays can benefit from use of the compounds disclosed herein. |
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| 16 | IMPROVED METHOD OF LIVE BLOOD CELL ANALYSIS | PCT/US1984001673 | 1984-10-17 | WO1985001960A1 | 1985-05-09 | LIVCELL ANALYSIS, INC.; PRIVITERA, James, R. |
| A method of performing an analysis of a live blood cell preparation to detect and identify pathological conditions that are manifested by pathophysiological alterations of blood cells. The determination of cellular aberrations and inclusions is determined on a live blood cell preparation that are subjected to dark-field microscopic examination immediately after it has been withdrawn from a patient. The method utilizes a microscope with a video camera attachment thereby permitting simultaneously viewing of the live blood cell preparation by both the attending technician and the patient. Further, the invention involves a method of treatment that utilizes natural substances, vitamin and mineral supplements, and modified diets. | ||||||
| 17 | Illumination structure for medical instrument | JP2008106949 | 2008-04-16 | JP2009254566A | 2009-11-05 | YAMAZAKI TOSHIO |
| PROBLEM TO BE SOLVED: To provide an illumination structure for a medical instrument for reliably illuminating an inside of a cavity-shaped part at a short distance. SOLUTION: Since a light transmission lens part is provided on the surface of a brain retractor 3, where an optical fiber distal end led inside the brain retractor 3 faces, the transmission light lens part of the brain retractor 3 itself emits light so that the illumination light B reliably lights the inside of the cavity-shaped part 2 at a short distance. Therefore, by increasing magnification of a surgical microscope 4, the inside of the cavity-shaped part 2 is lighted by the illumination light B from the brain retractor 3 at a short distance even when the brightness in the field of view is insufficient only with illumination light A from the surgical microscope 4 (the larger the magnification of the microscope becomes, the darker a field of view becomes). Thus, a state inside the cavity-shaped part 2 is reliably observed by the surgical microscope 4 in a bright field of view. COPYRIGHT: (C)2010,JPO&INPIT | ||||||
| 18 | Ophthalmological surgical microscope | JP2003163931 | 2003-06-09 | JP2005000214A | 2005-01-06 | KITAJIMA NOBUAKI |
| <P>PROBLEM TO BE SOLVED: To provide an ophthalmological surgical microscope that provides a good field of vision. <P>SOLUTION: The ophthalmological surgical microscope tilts the optical axis O<SB>f</SB>of a front lens 40 by nearly half the angle formed by an observation axis O and a lighting axis L with respect to the observation axis O. Consequently, two reflected images of the outgoing pupil of a lighting optical system attributed to the upper and lower refraction faces of the front lens 40 appear to be nearly superposed on each other, making the observable area in the field of vision expandable. A shading member 16 is arranged in the position conjugate to the center A of the incoming pupil 12a of an observation optical system 12. Since the shading area 16c of the shading member 16 shades a part of lighting light for microscoping ophthalmic surgery corresponding to the position where the reflected images are observed. The area of the reflected images are observed as scotoma, it is easy to observe the peripheries of the images. <P>COPYRIGHT: (C)2005,JPO&NCIPI | ||||||
| 19 | System and method for correlating photoreceptor pigmented film layer to electrical performance | US12490183 | 2009-06-23 | US08045156B2 | 2011-10-25 | Dante M. Pietrantoni; Kamran U. Zaman; Jonathan H. Herko; Scott J. Griffin; Michael S. Roetker |
| The presently disclosed embodiments are directed to a system and method for obtaining spectra of highly scattering pigmented layers and providing a spectral reflection ratio, which can be correlated to photoreceptor electrical performance. The present embodiments employ the use of dark field microscopy in combination with a noise reducing normalization technique to provide real-time production adjustments to optimize photoreceptor characteristics and/or performance. | ||||||
| 20 | SYSTEM AND METHOD FOR CORRELATING PHOTORECEPTOR PIGMENTED FILM LAYER TO ELECTRICAL PERFORMANCE | US12490183 | 2009-06-23 | US20100321689A1 | 2010-12-23 | Dante M. Pietrantoni; Kamran U. Zaman; Jonathan H. Herko; Scott J. Griffin; Miichael S. Roetker |
| The presently disclosed embodiments are directed to a system and method for obtaining spectra of highly scattering pigmented layers and providing a spectral reflection ratio, which can be correlated to photoreceptor electrical performance. The present embodiments employ the use of dark field microscopy in combination with a noise reducing normalization technique to provide real-time production adjustments to optimize photoreceptor characteristics and/or performance. | ||||||
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