METHOD FOR DETECTING COLIFORM BACTERIA CONTAINED IN MILK

申请号 EP14870998 申请日 2014-12-17 公开(公告)号 EP3085769A4 公开(公告)日 2017-07-19
申请人 ASAHI CHEMICAL IND; 发明人 MAEHANA KOJI; MATSUYAMA KENJI;
摘要 The object is to provide a lysis method, lysis treatment solution, detection method using an immunochromatographic device, and detection kit comprising an immunochromatographic device for detecting whether causative bacteria of mastitis are coliform bacteria or not by using milk of a livestock animal. There is provided a method for lysing coliform bacteria, which comprises the step of mixing a lysis agent containing a lytic enzyme, and at least one kind of anionic surfactant, and preferably further containing at least one kind of nonionic surfactant, with milk obtained form a livestock animal to lyse coliform bacteria existing in the milk. The lytic enzyme is preferably lysozyme.
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