| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 461 | Non-reducing saccharide-forming enzyme, trehalose-releasing enzyme, and process for producing saccharides using the enzymes | EP99307220.6 | 1999-09-13 | EP0990704A2 | 2000-04-05 | Yamamoto, Takuo; Maruta, Kazuhiko; Kubota, Michio; Fukuda, Shigeharu; Miyake, Toshio |
A non-reducing saccharide-forming enzyme and a trehalose-releasing enzyme, which have an optimum temperature in a medium temperature range, i.e., a temperature of over 40 or 45°C but below 60°C; and an optimum pH in an acid pH range, i.e., a pH of less than 7. The two-types of enzymes can be obtained in a desired amount, for example, by culturing in a nutrient culture medium microorganisms capable of producing the enzymes or by recombinant DNA technology. |
||||||
| 462 | Polynucleotides encoding trehalose-6-phosphate phosphatase and methods of use thereof | US13549794 | 2012-07-16 | US09121033B2 | 2015-09-01 | Kateri Duncan; Michael L. Nuccio; Weining Gu |
| The present invention relates generally to the field of molecular biology and regards various polynucleotides, polypeptides and methods that may be employed to enhance yield in transgenic plants. Specifically the transgenic plants may exhibit any one of the traits consisting of increased yield, increased tolerance to abiotic stress, increased cell growth and increased nutrient use efficiency. | ||||||
| 463 | Recombinant thermostable enzyme for converting maltose into trehalose from Thermus aquaticus | US537002 | 1995-09-29 | US5773282A | 1998-06-30 | Keiji Tsusaki; Michio Kubota; Toshiyuki Sugimoto |
| Disclosed are a recombinant thermostable enzyme, which converts maltose into trehalose and is stable up to a temperature of about 80.degree. C. even when incubated at pH 7.0 for 60 min, a preparation of the enzyme, a DNA encoding the enzyme, a recombinant DNA containing the DNA, a transformant, and an enzymatic conversion method of maltose by using the enzyme. | ||||||
| 464 | Recombinant enzyme for converting maltose into trehalose from pimelobacter sp. | US528199 | 1995-09-14 | US5763228A | 1998-06-09 | Michio Kubota; Keiji Tsusaki; Toshiyuki Sugimoto |
| A recombinant enzyme, having a molecular weight of about 57,000-67,000 daltons on SDS-PAGE and a pI of about 4.1-5.1 on isoelectrophoresis, which converts maltose into trehalose and vice versa. Depending on the enzymatic conditions, the enzyme forms about 70 w/w % of trehalose when acts on maltose, while about 20 w/w % of maltose when acts on trehalose. The culture of a transformant, prepared by introducing into a host a recombinant DNA containing a DNA coding for the enzyme and a self-replicable vector, facilitates the industrial-scale production of trehalose. | ||||||
| 465 | "Alga species lobsphaera TM-33 (ATCC 75630) which is useful for preparing trehalase" | US231318 | 1994-04-22 | US5529927A | 1996-06-25 | Sumio Kitahata; Hirofumi Nakano; Tsutomu Washino; Masamitsu Moriwaki |
| Novel trehalase having the properties that it hydrolyzes .alpha.,.alpha.'-trehalose, 2,2'-dideoxy-.alpha.,.alpha.'-trehalose and 2-deoxy-.alpha.,.alpha.'-trehalose into the respective constituting sugars while it does not act on neotrehalose, lactose, maltose, celobiose and sucrose; its optimum pH is 5 to 6; its optimum temperature is 65.degree. C.; it is stable against heating up to 65.degree. C.; its molecular weight as measured by a gel filtration is 400,000 to 500,000 while the molecular weight of the subunit as measured by a sodium dodecylsulfate--polyacrylamide gel electrophoresis is 180,000 to 250,000; its isoelectric point as measured by an isoelectricfocusing is 2.7; and it is a glycoprotein. | ||||||
| 466 | PROCESS FOR PRODUCING ETHANOL FROM A HYDROLYSATE OF THE HEMICELLULOSE FRACTION OF SUGARCANE BAGASSE IN A PRESS REACTOR | US12667369 | 2007-07-03 | US20100273228A1 | 2010-10-28 | Lidia Maria Melo Sant'Anna; Nei Pereira; Gabriel Jamie Vargas Bitancur; Juliana Vaz Bevilaqua; Absai da Conceicao Gomes; Emerson Pires Menezes |
| The invention is a process for producing ethanol from sugarcane bagasse, the principal steps of which are mild sulphuric acid hydrolysis of the hemicellulose fraction of the sugarcane bagasse, followed by extraction of the hydrolysate and then fermentation thereof with the yeast Pichia stipitis. The process can be carried out with different solid : liquid ratios, and provides a step of acclimatizing the Pichia stipitis yeast, which results in a greater rate of ethanol production. The process takes place in a press reactor specially designed for this purpose, which allows more efficient extraction of the hydrolysate and, as a consequence, better process performance. | ||||||
| 467 | Methods for the identification of inhibitors of Trehalose-6-Phosphate Synthase as antibiotics | US10703292 | 2003-11-07 | US20040146960A1 | 2004-07-29 | Blaise Darveaux; Sanjoy Mahanty; Ryan Heiniger; Amy Covington; Huaqin Pan; Rex Tarpey; Jeffrey Shuster; Matthew M. Tanzer; Lisbeth Hamer; Kiichi Adachi; Todd DeZwaan; Sze-Chung Lo; Maria Victoria Montenegro-Chamorro; Sheryl Frank |
| The present inventors have discovered that Trehalose-6-Phosphate Synthase is essential for normal fungal pathogenicity. Specifically, the inhibition of Trehalose-6-Phosphate Synthase gene expression in fungi results in reduced pathogenicity (i.e. smaller, non-viable lesions). Thus, Trehalose-6-Phosphate Synthase can be used as a target for the identification of antibiotics, preferably antifungals. Accordingly, the present invention provides methods for the identification of compounds that inhibit Trehalose-6-Phosphate Synthase expression or activity. The methods of the invention are useful for the identification of antibiotics, preferably antifungals. | ||||||
| 468 | USE OF SEA CUCUMBER GLYCOSAMINOGLYCAN IN PREPARING MEDICINE FOR PREVENTION AND TREATMENT OF THROMBOEMBOLIC DISEASE | US14890854 | 2014-05-07 | US20160082051A1 | 2016-03-24 | Zhiguo Wang; Quanhai Liu; Xuehai Wu |
| The present invention discloses use of sea cucumber glycosaminoglycan in the preparation of drugs. In particular, the present invention relates to use of medical use of sea cucumber glycosaminoglycan, and more particularly to use of depolymerized sea cucumber glycosaminoglycan or natural molecular segments of sea cucumber glycosaminoglycan with a weight average molecular weight greater than 54,500 Da in the preparation of a drug for the prevention and treatment of thromboembolic diseases. Thromboembolic diseases include atherosclerotic thrombotic diseases, venous thromboembolic diseases, hypercoagulable states and postoperative thrombosis or treatment of postoperative thrombi. The present invention has a wide treatment window for thromboembolic diseases, has a higher level of safety, and has good development and research value. | ||||||
| 469 | Sequence-determined DNA fragments encoding trehalose-6P phosphatase proteins | US14301826 | 2014-06-11 | US09068173B2 | 2015-06-30 | Nickolai Alexandrov; Vyacheslav Brover; Kenneth Feldmann |
| The present invention provides DNA molecules that constitute fragments of the genome of a plant, and polypeptides encoded thereby. The DNA molecules are useful for specifying a gene product in cells, either as a promoter or as a protein coding sequence or as an UTR or as a 3′ termination sequence, and are also useful in controlling the behavior of a gene in the chromosome, in controlling the expression of a gene or as tools for genetic mapping, recognizing or isolating identical or related DNA fragments, or identification of a particular individual organism, or for clustering of a group of organisms with a common trait. | ||||||
| 470 | OtsA gene encoding trehalose-6-phosphate synthase from a coryneform bacterium | US10637551 | 2003-08-11 | US07307160B1 | 2007-12-11 | Hiromi Ohtaki; Jun Nakamura; Hiroshi Izui; Tsuyoshi Nakamatsu |
| L-Glutamic acid is produced by culturing a coryneform bacterium having L-glutamic acid producing ability, in which trehalose synthesis ability is decreased or deleted by, for example, disrupting the otsA gene derived from a coryneform bacterium source, coding for trehalose-6-phosphate synthase, to produce and accumulate L-glutamic acid in the medium, and collecting the L-glutamic acid from the medium. | ||||||
| 471 | Immobilized acetylcholinesterase stabilized by a film of gelatin or albumin containing trehalose | US405816 | 1995-03-16 | US5624831A | 1997-04-29 | Nguyen Vu Khue; Phillippe Poindron; Roland Maes |
| Acetylcholinesterase and/or a receptor of acetylcholinesterase is immobilized on a solid support and stabilized by covering with a protective film of gelatin and/or albumin containing trehalose. The film is applied by covering the immobilized acetylcholinesterase and/or receptor of acetylcholinesterase with a layer of a gel-forming solution of gelatin or albumin in an evaporable solvent containing dissolved trehalose, and evaporating the solvent to leave the film. The film provides stability against dry heat, organic solvents, proteases and changes in pH. Other additives such as polyhydric alcohols, organic solvents, polymers and/or ionic and non-ionic components may be present to increase stabilization. A diagnostic kit or chromatography column may be formed containing the stabilized immobilized acetylcholinesterase and/or receptor of acetylcholinesterase. | ||||||
| 472 | Trehalase and a method of manufacturing sugars by said enzyme | EP94105701.0 | 1994-04-13 | EP0661381A3 | 1995-11-29 | Kitahata, Sumio; Nakano, Hirofumi; Washino, Tsutomu; Moriwaki, Masamitsu |
Novel trehalase having the properties that it hydrolyzes α,α'-trehalose, 2,2'-dideoxy-α,α'-trehalose and 2-deoxy-α,α'-trehalose into the respective constituting sugars while it does not act on neotrehalose, lactose, maltose, celobiose and sucrose; its optimum pH is 5 to 6; its optimum temperature is 65°C; it is stable against heating up to 65°C; its molecular weight as measured by a gel filtration is 400,000 to 500,000 while the molecular weight of the subunit as measured by a sodium dodecylsulfate - polyacrylamide gel electrophoresis is 180,000 to 250,000; its isoelectric point as measured by an isoelectricfocusing is 2.7; and it is a glycoprotein. |
||||||
| 473 | Trehalase and a method of manufacturing sugars by said enzyme | EP94105701.0 | 1994-04-13 | EP0661381A2 | 1995-07-05 | Kitahata, Sumio; Nakano, Hirofumi; Washino, Tsutomu; Moriwaki, Masamitsu |
Novel trehalase having the properties that it hydrolyzes α,α'-trehalose, 2,2'-dideoxy-α,α'-trehalose and 2-deoxy-α,α'-trehalose into the respective constituting sugars while it does not act on neotrehalose, lactose, maltose, celobiose and sucrose; its optimum pH is 5 to 6; its optimum temperature is 65°C; it is stable against heating up to 65°C; its molecular weight as measured by a gel filtration is 400,000 to 500,000 while the molecular weight of the subunit as measured by a sodium dodecylsulfate - polyacrylamide gel electrophoresis is 180,000 to 250,000; its isoelectric point as measured by an isoelectricfocusing is 2.7; and it is a glycoprotein. |
||||||
| 474 | Non-reducing saccharide-forming enzyme, trehalose-releasing enzyme, and process for producing saccharides using the enzymes | US11564493 | 2006-11-29 | US07575900B2 | 2009-08-18 | Takuo Yamamoto; Kazuhiko Maruta; Michio Kubota; Shigeharu Fukuda; Toshio Miyake |
| A non-reducing saccharide-forming enzyme and a trehalose-releasing enzyme, which have an optimum temperature in a medium temperature range, i.e., a temperature of over 40 or 45° C. but below 60° C.; and an optimum pH in an acid pH range, i.e., a pH of less than 7. The two-types of enzymes can be obtained in a desired amount, for example, by culturing in a nutrient culture medium microorganisms capable of producing the enzymes or by recombinant DNA technology. | ||||||
| 475 | USE OF TREHALOSE-6-PHOSPHATE SYNTHASE TO MODULATE PLANT GROWTH | EP07703096.3 | 2007-01-29 | EP1989312A1 | 2008-11-12 | LEYMAN, Barbara; RAMON, Matthew; ROLLAND, Filip; THEVELEIN, Johan; VAN DIJCK, Patrick; VANDESTEENE, Lies |
| The present invention relates to the use of trehalose-6-phosphate synthase to modulate plant growth. More specifically, it relates to the use of a class Il trehalose-6-phosphate synthase, comprising both a synthase and a phosphatase-like part to modulate plant growth. Preferably, the activity of trehalose-6-phosphate synthase is downregulated to obtain an increased plant biomass yield. | ||||||
| 476 | USE OF TREHALOSE-6-PHOSPHATE SYNTHASE TO MODULATE PLANT GROWTH | EP07703096.3 | 2007-01-29 | EP1989312B1 | 2011-10-19 | LEYMAN, Barbara; RAMON, Matthew; ROLLAND, Filip; THEVELEIN, Johan; VAN DIJCK, Patrick; VANDESTEENE, Lies |
| The present invention relates to the use of trehalose-6-phosphate synthase to modulate plant growth. More specifically, it relates to the use of a class Il trehalose-6-phosphate synthase, comprising both a synthase and a phosphatase-like part to modulate plant growth. Preferably, the activity of trehalose-6-phosphate synthase is downregulated to obtain an increased plant biomass yield. | ||||||
| 477 | Trehalose releasing enzyme, DNA encoding therefor, their preparation and uses | EP95301474.3 | 1995-03-07 | EP0671470B9 | 2004-11-03 | Kubota, Michio; Tsusaki, Keiji; Hattori, Kazuko; Sugimoto, Toshiyuki |
| 478 | POLYPEPTIDES HAVING TREHALASE ACTIVITY AND THE USE THEREOF IN PROCESS OF PRODUCING FERMENTATION PRODUCTS | EP16734780.6 | 2016-06-13 | EP3310910A1 | 2018-04-25 | KANG, Zhengfang; SCHNORR, Kirk Matthew; SKOV, Lars Kobberøe |
| The present invention relates to polypeptides having trehalase activity. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using a trehalase of the invention, in particular a process of producing a fermentation product, such as ethanol. | ||||||
| 479 | USE OF TREHALASE GENES TO CONFER NEMATODE RESISTANCE TO PLANTS | EP08708688.0 | 2008-02-05 | EP2111456A1 | 2009-10-28 | WIIG, Aaron; HUANG, Xiang; CHAUDHURI, Sumita |
| The invention provides transgenic plants that exhibit increased resistance to nematode infection by virtue of overexpression of a gene that encodes trehalase in nematode-induced syncytia. Expression vectors comprising trehalase-encoding polynucleotides and methods of employing such vectors to increase nematode resistance of plants are also provided. | ||||||
| 480 | Maltose-trehalose converting enzyme, and preparation and uses thereof | EP94305342.1 | 1994-07-20 | EP0636693B1 | 2005-12-28 | Nishimoto, Tomoyuki; Chaen, Hiroto; Sugimoto, Toshiyuki; Miyake, Toshio |
QQ群二维码
意见反馈
意见反馈