Exponential decay time constant measurement using frequency of offset-phase locked loop: system and method |
申请号 |
EP88304136.0
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申请日 |
1988-05-06 |
公开(公告)号 |
EP0294938A3 |
公开(公告)日 |
1990-03-28 |
申请人 |
Hewlett-Packard Company;
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发明人 |
Dukes, John Noland;
Carlsen, William F.,Jr.;
Pittaro, Richard J.;
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摘要 |
Oxygen determination based on luminescence quenching of fluorescent dye is effected by using the frequency output of an offset-phase locked loop (15) to calculate the time constant for the exponential decay of fluorescence. An offset phase angle between a periodic stimulus signal used to excite the dye and a response signal based on fluorescence detection is predetermined to optimize signal-to-noise ration for a wide range of time constants. An offset-phase locked loop (15) is used to vary the frequency of a periodic stimulus signal until the predetermined phase relationship is established, and the frequency forms a measure of the decay rate. Where the stimulus and response signals are substantially sinusoidal, the offset phase angle is ideally about 49.3°, although substantially optimal performance is achieved using a more conveniently generated 45°. The 45° angle offset can also be used with a square-wave stimulus signal. |
权利要求 |
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说明书全文 |
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