BIOREACTOR FOR IN VITRO PLANT CULTURE

申请号 EP13897207 申请日 2013-11-11 公开(公告)号 EP3069591A4 公开(公告)日 2017-05-17
申请人 FIBRIA CELULOSE SA; 发明人 PENCHEL RICARDO MIGUEL; REIS JOCEMAR PALAURO DOS; OLIVEIRA MILA LIPARIZE DE;
摘要 The present utility model aims to provide a bioreactor for the in vitro cultivation of plants of the temporary immersion type comprising: (i) an upper container (2) with transparent walls for the material being propagated, preferably explants of eucalyptus, said container (2) being provided with (a) gas exchange diffuser to the external environment, (b) humidifier (12), and (c) artificial illumination (14); (ii) a lower container (3) with transparent walls for the liquid nutrient medium, having an aluminum tray (15) and a point (13) of water entry and/or nutrient medium, located in the bottom of said container (3); (iii) points (4) of injection/removal of air/oxygen/carbon dioxide located in the transparent lid (5); (iv) point (11) of additional injection of carbon dioxide with a drip line, close to the bottom of said upper container (2); (v) means (6) of hermetic connection between the upper and lower containers for supply/drainage of the nutrient medium to and from the upper container; (vi) a screen support (7) for the material to be propagated, with the possibility to exchange screens of different meshes; (vii) means (8) for locking the lid to hermetically close the upper container; (viii) means (9) for locking the lid to hermetically close the lower container; and (ix) means (10) for pneumatic drive of the liquid nutrient medium between said upper container and said lower container.
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