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Killed vaccine for infectious porcine atrophic rhinitis and process for preparing the same

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专利汇可以提供Killed vaccine for infectious porcine atrophic rhinitis and process for preparing the same专利检索,专利查询,专利分析的服务。并且A killed vaccine for infectious porcine atrophic rhinitis which is prepared from a Phase I organism contained in the microorganism Bordetella bronchiseptica, and a process for preparing said vaccine. The Phase I organism of said microorganism is a Gram-negative germ, which possesses a K antigen and an antigen capable of giving to mice a protective potency against mouse intracerebral infection and exhibits on a blood agar medium a coccoid or coccobacillary form having capsules or envelopes and having no or slight flagella, and which has such properties as showing strong agglutination to anti-K serum but little or slight agglutination to anti-O serum, showing high virulence when injected into mouse brains, and showing significant necrotoxicity when intracutaneously injected into guinea-pigs. The above-mentioned killed vaccine is prepared by culturing a K antigen-possessing Phase I organism of Bordetella bronchiseptica in a base medium of nutrient agar or nutrient broth which has been incorporated with 0.5 to 1.0 percent of Casamino acid, 0.05 to 1.0 percent of yeast extract, and 0.1 to 0.5 percent of activated carbon powder or 0.3 to 1.0 percent of anion exchange resin, and then killing the resulting cells or inactivating a lysate of the cells.,下面是Killed vaccine for infectious porcine atrophic rhinitis and process for preparing the same专利的具体信息内容。

1. A KILLED VACCINE FOR INFECTIOUS PORCINE ATROPHIC RHINITIS INCORPORATING AN ALUMINA GEL OR OIL ADJUVANT WHICH IS PREPARED FROM A PHASE I ORGANISUM CONTAINED IN THE MICROORGANISUM BORDETELLA BRONCHISEPTICA IN A SOLID OR LIQUID MEDIUM, AND THEN KILLING THE RESULTING CELLS OR INACTIVATING A LYSATE OF THE CELLS SAID PHASE I ORGANISM BEING A GRAM-NEGATIVE GERM WHICH POSESSES A K ANTIGEN AND AN ANTIGEN CAPABLE OF GIVING TO MICE A PROTECTIVE POTENCY AGANST MOUSE INTERACEREBRAL INFECTION AND EXHIBITS ON A BLOOD AGAR MEDIUM A COCCOID OR COCCOBACILLAYER FROM HAVING A CAPSULES OR ENVELOPES AND HAVING NO OR SLIGHT FLAGELLA, AND WHICH HAS SUCH PROPERTIES AS SHOWING STRONG AGGULTINATION TO ANTI-K SERUM BUT LITTLE OR SLIGHT AGGLUTINATION TO ANTI-O SERUM, SHOWING HIGH VIRULENCE WHEN INJECTED INTO MOUSE BRAINS, AND SHOWING SIGNIFICANT NECROTOXICITY WHEN INTRACUTANEOUSLY INJECTED INTO GUNEA-PIGS.
2. In the art of immunizing delivered piglets, pregnant mother pigs and adult pigs, actively or passively, against infectious porcine atrophic rhinitis caused by Bordetella bronchiseptica, the improvement which consists of the step of immunizing said pigs with a killed vaccine prepared from a Phase I organism contained in the microorganism Brodetella bronchiseptica, said Phase I organism being a Gram-negative germ which possesses a K antigen and an antigen capable of giving to mice a protective potency against mouse intracerebral infection and exhibits on a blood agar medium a coccoid or coccobacillary form having capsules or envelopes and having no or slight flagella, and which has such properties as showing strong agglutination to anti-K serum but little or slight agglutination to anti-O serum, showing high virulence when injected into mouse brains, and showing significant necrotoxicity when intracutaneously injected into guinea-pigs.
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