| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 181 | 用于链霉素耐药结核分枝杆菌的核酸快速检测试剂盒及其检测方法 | CN202110150109.3 | 2021-02-03 | CN112725487A | 2021-04-30 | 张国良; 刘鹏; 刘磊; 王鑫杰; 刘馨怡; 董谦; 张锦萍 |
| 本发明公开了一种用于链霉素耐药结核分枝杆菌的核酸快速检测试剂盒及其检测方法，针对rpsl Wild type基因、rpsl K43R和rpsl K88R耐药突变基因片段3个基因，设计一系列特异性crRNA,可灵敏、特异检测到链霉素耐药结核分枝杆菌核酸。本发明系首次采用Cas12a RR检测链霉素耐药结核分枝杆菌，具有灵敏度高、特异性强、耗时短、高通量、肉眼可直接判读和不依赖大型实验设备等优势。这些优势使得本发明开发的基于Cas12a RR荧光法方法方便用于临床一线对链霉素耐药结核分枝杆菌核酸的快速检测和鉴定诊断。 | ||||||
| 182 | 一种链霉素电化学-光电化学双方法比率适配体传感器的制备方法及应用 | CN201910800125.5 | 2019-08-28 | CN110618177A | 2019-12-27 | 刘东; 申秀丽; 由天艳 |
| 本发明属于生物传感检测技术领域，涉及一种链霉素电化学-光电化学双方法比率传感器的制备及应用。具体为首次提出一种基于电化学和光电化学两种方法的新型比率适配体传感器用于链霉素的特异性检测。本发明中，链霉素存在时，亚甲基蓝标记的链霉素适配体(简写为MB-Apt)可与其特异性结合并引起MB-Apt构象的变化使得MB-Apt脱离电极表面，进一步引起光电化学信号及电化学信号的双重变化。构建的传感器对链霉素的线性响应范围为3.0×10-8-1.0×10-4mol/L，检出限为1.0×10-8mol/L。本发明的电化学-光电化学双方法比率适配体传感器选择性好且灵敏度高，结合两种方法获得更高的准确度，为测定实际样品中链霉素提供了一种新型的传感平台。 | ||||||
| 183 | 一种同时测定普鲁卡因、青霉素和链霉素含量的液相色谱分析方法 | CN201711178591.1 | 2017-11-23 | CN107884498A | 2018-04-06 | 毕海林; 苏玲; 肖裕章; 张超; 袁启峰 |
| 本发明公开了一种同时测定普鲁卡因、青霉素和链霉素含量的液相色谱分析方法，该方法先分别配制普鲁卡因、青霉素和链霉素对照品标准溶液，然后配制含普鲁卡因、青霉素和/或链霉素成分的供试品溶液，再使用液相色谱得到的液相色谱分别绘制普鲁卡因、青霉素和链霉素的标准曲线，最后检测供试品溶液，并利用普鲁卡因、青霉素和链霉素的标准曲线来计算普鲁卡因、青霉素/或链霉素的含量。本发明提供方法能准确检测普鲁卡因、青霉素、链霉素的含量，方法专属性强、精密度高、稳定性好、准确度高，适用于兽药行业中的普鲁卡因青霉素—链霉素单方与复方产品的质量控制，可有效的节约检测成本和时间。 | ||||||
| 184 | 一种基于核酸适配体和量子点同时检测链霉素氯霉素四环素三种抗生素残留的方法 | CN201510751684.3 | 2015-11-09 | CN105301085B | 2017-10-27 | 周楠迪; 薛璟珂; 田亚平; 刘晶 |
| 一种基于核酸适配体和量子点同时检测链霉素氯霉素四环素三种抗生素残留的方法，属于分析化学技术领域。本发明设计合成了三段互补cDNA1序列，分别与三种抗生素适配体DNA序列互补，同时还与三种抗生素各自的捕获探针DNA序列和互补cDNA2序列部分互补。三种cDNA1首先和各自互补的适配体DNA杂交形成双链DNA，当样品中存在目标抗生素时，适配体DNA优先与其对应的抗生素结合使双链DNA去杂交并释放出cDNA1。游离cDNA1与修饰在金电极表面的捕获探针DNA结合，三种量子点修饰的cDNA2与cDNA1的另一端杂交。金电极表面的三种量子点经酸溶后会产生与目标抗生素对应的溶出伏安峰。利用峰电流值的变化和抗生素浓度之间的关系实现对三种抗生素的检测。该方法具有良好的重复性、稳定性和高灵敏度，可用于对牛奶等样品中的三种抗生素残留进行直接测定。 | ||||||
| 185 | 检测大观霉素、链霉素、庆大霉素和新霉素的试纸条 | CN201220510206.5 | 2012-09-29 | CN203069593U | 2013-07-17 | 万宇平; 罗晓琴; 刘叶; 聂雯莹; 杨秀贤; 郝士元; 陈娟; 韩京朋 |
| 本实用新型公开了一种检测大观霉素、链霉素、庆大霉素和新霉素的试纸条。试纸条包括微孔条、微孔试剂、试纸条和微孔塞，所述试纸由样品吸收垫、反应膜、吸水垫、保护膜、底板依次连接组成，所述反应膜上包括四条检测线和一条质控线，四条检测线分别包被有大观霉素-载体蛋白偶联物、链霉素-载体蛋白偶联物、庆大霉素-载体蛋白偶联物和新霉素-载体蛋白偶联物，质控线包被有羊抗鼠抗抗体。用本实用新型试纸条检测大观霉素、链霉素、庆大霉素和新霉素的方法，简便、快速、直观、准确、便于携带、适用范围广、成本低、易推广使用。 | ||||||
| 186 | 检测螺旋霉素、链霉素、庆大霉素、新霉素的试纸条 | CN201220509664.7 | 2012-09-29 | CN203069592U | 2013-07-17 | 何方洋; 冯才伟; 杨秀贤; 刘叶; 吴小胜; 赵正苗; 汪善良 |
| 本实用新型提供了一种检测螺旋霉素、链霉素、庆大霉素、新霉素的试纸条，试纸条包括微孔试剂和试纸，试纸由底板、附着在底板上依次紧密相连的样品吸收垫、反应膜、吸水垫和保护膜组成，螺旋霉素单克隆抗体-胶体金标记物、链霉素单克隆抗体-胶体金标记物、庆大霉素单克隆抗体-胶体金标记物、新霉素单克隆抗体-胶体金标记物冻干在微孔试剂中，反应膜上具有包被有螺旋霉素半抗原-载体蛋白偶联物、链霉素半抗原-载体蛋白偶联物、庆大霉素半抗原-载体蛋白偶联物、新霉素半抗原-载体蛋白偶联物构成的四条检测线印迹和包被羊抗鼠抗抗体构成的质控线印迹。本实用新型试纸条具有便携性好，灵敏度高，检测时间短等特点。 | ||||||
| 187 | Preparation of chain terpenediol | JP7498477 | 1977-06-24 | JPS549208A | 1979-01-24 | MURATA ATSUO; TSUCHIYA SHIYUUJI; SUZUKI HIDEO; IKEDA HISAO |
| PURPOSE: To prepare a chain terpenediol useful as perfumes and raw material of perfumes, selectively, by reducing specific hydroxylamine under specific hydrogen pressure in the presence of a catalyst such as reducing copper, etc. COPYRIGHT: (C)1979,JPO&Japio | ||||||
| 188 | Preparation of chain terpenediol | JP3130577 | 1977-03-22 | JPS53116311A | 1978-10-11 | MURATA ATSUO; IKEDA HISAO; SUZUKI HIDEO; TSUCHIYA SHIYUUJI |
| PURPOSE: To obtain a chain terpendediol selectively without reduction and shift of double bonds, by the catalytic reductive cleavage reaction of a hydroxylamine derivative of a chain unsaturated hydrocarbon in the presence of a specific catalyst. COPYRIGHT: (C)1978,JPO&Japio | ||||||
| 189 | 一种基于属特异性引物-PCR结合链霉素抗性平板特异性分离鞘氨醇单胞菌的方法及其专用引物 | CN201210125014.7 | 2012-04-25 | CN102643918A | 2012-08-22 | 李慧; 周丽莎; 张颖; 徐慧; 韩斯琴; 史荣久; 杨伟超 |
| 本发明涉及微生物学技术和分子生物学技术交叉领域，具体的说一种基于属特异性引物-PCR结合链霉素抗性平板特异性分离鞘氨醇单胞菌的方法及其专用引物。具体为，将待测样品悬液涂布含链霉素的LB培养基固体平板，挑取黄色菌落反复划线分纯，经液体培养后，提取纯菌株基因组DNA，采用鞘氨醇单胞菌属特异性引物PCR扩增16SrDNA片段，存在阳性扩增且扩增得到504bpPCR产物的片段即为鞘氨醇单胞菌。采用本发明提供的属特异性引物扩增结合链霉素抗性平板法能够特异性的从土壤中直接分离可培养的鞘氨醇单胞菌，针对这一特殊种属进行计数，同时获得可培养的鞘氨醇单胞菌菌株资源。 | ||||||
| 190 | 스트렙토그라민 Ａ 유도체 및 이를 포함하는 항생제 | KR1020130054110 | 2013-05-14 | KR101516386B1 | 2015-05-04 | 박제원 |
| 본 발명은 항생 활성을 갖는 스트렙토그라민 A 유도체, 상기 스트렙토그라민 A 유도체 또는 그의 약제학적으로 허용되는 염을 포함하는 항생 조성물, 상기 스트렙토그라민 A 유도체의 제조 방법에 관한 것이다.
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| 191 | 정제형스트렙토그라민및그의제법 | KR1019940002667 | 1994-02-15 | KR100333185B1 | 2002-08-21 | 바스칼앤거; 버트란보나보; 알랭깔레; 빠뜨릭르페브르 |
| 하기 구조식의 스트렙토그라민의 하나 이상의 B군 성분 ; (상기식에서, A1은 하기 구조식: 의 라디칼이고, 이때 R'는 수소원자 또는 히드록시 라디칼이며, Y는 수소원자, 메틸아미노 라디칼 또는 디메틸아미노 라디칼이고, R은 에틸 라디칼이거나, R'가 수소 원자인 경우 R은 또한 메틸 라디칼이고, R1 및 R2는 각각 수소원자이거나, 또는 A1은 하기 구조식; 의 라디칼이고, R은 이소 부틸 라디칼이고, R1은 히드록실 라디칼이며 R2는 메틸 라디칼임), 및 하기 구조식의 스트렙토그라민의 하나이상의 A군 부성분; (상기식에서, R"는 수소 원자, 또는 메틸 또는 에틸 라디칼임)의 배합물로 구성된, 공결정물, 공침전물 또는 미세 분쇄 물리적 혼합물 형태의 정제형 스트렙토그라민은 유용한 항균성 활성을 갖는다. | ||||||
| 192 | 스트렙토마이신- 민감성 유전자를 함유한는 플라즈미드 벡터 | KR1019880012513 | 1988-09-28 | KR1019890005271A | 1989-05-13 | 토바마리; 하지모찌다모쭈 |
| 내용 없음 | ||||||
| 193 | Streptomycin salts | US39420953 | 1953-11-24 | US2783220A | 1957-02-26 | MILTON WOLF |
| 194 | Cellular toxin product formed by covalent bond of lysine a chain with antibody | JP12525779 | 1979-09-28 | JPS5549321A | 1980-04-09 | GUI BOASHIN; FURANTSU JIYANSEN; PIEERU GUROSU |
| 195 | STREPTOSPIROLE DERIVATIVES | EP09777218.0 | 2009-07-16 | EP2321322A1 | 2011-05-18 | WINK, Joachim; GERLITZ, Martin; OLIVAN, Hélène; KURZ, Michael; DUFOUR-SCHROIF, Cosima |
| The invention relates to streptospirole derivatives of the general formula (I), wherein R 1, R 2, R 3, X 1, X 2, Y 1 and Y 2 are as defined herein, a process for the preparation of said compounds by fermenting the microorganism Streptomyces sp. ST 108140 (DSM 19369) and optionally derivatizing the compounds produced by said microorganism, a pharmaceutical composition comprising at least one compound of the formula (I), and the use of a compound of the formula (I) for the preparation of a medicament for the treatment and/or prophylaxis of bacterial infections. | ||||||
| 196 | Farbmarker in Streptomyceten-Plasmiden | EP87111509.3 | 1987-08-08 | EP0257417B1 | 1992-05-13 | Muth, Günter; Wohlleben, Wolfgang, Dr.; Pühler, Alfred, Prof. Dr.; Wöhner, Gerhard, Dr.; Marquardt, Rüdiger, Dr. |
| 197 | Production of straight-chain type amylose | JP11013194 | 1994-04-27 | JPH07289278A | 1995-11-07 | MIYAMOTO JINICHI; MAEDA KAZUHIRO; FUJIMORI MASAHIRO; KAWAMURA KICHIYA |
| PURPOSE: To efficiencyly obtain an amylose useful as a raw material for adhesives, dispersing agents, filters, biodegradable resins, etc., by reacting glucose-1-phosphates and maltodextrin with a phosphorylase immobilized on a porous chitosan carrier. CONSTITUTION: This method for producing a straight-chain type amylose is to immerse a porous chitosan carrier in a buffer solution, sufficiently equilibrate the carrier, as necessary, carry out the deaeration treatment, then add the resultant carrier into a solution of the glutaraldehyde at 1-5wt.% concentration, shake the mixture at ambient temperature for 1-2hr, adsorb the glutaraldehyde on the carrier, thoroughly wash the carrier adsorbing the glutaraldehyde with water, then add the washed carrier into a buffer solution of a phosphorylase, allow the resultant mixture to stand at ambient temperature for 1-2hr and use the resultant immobilized enzyme in which the phosphorylase is immobilized as an enzyme,in a method for producing the amylose from glucose 1-phosphate or its salt and maltodextrin using the phosphorylase. Thereby, the immobilized enzyme is repetitively used to efficiently afford the straight-chain type amylose having a high polymerization degree at a low cost. COPYRIGHT: (C)1995,JPO | ||||||
| 198 | Chain terpene compound | JP16243990 | 1990-06-20 | JPH0454141A | 1992-02-21 | TAKAYANAGI HISAO; KITANO YASUNORI; MORINAKA YASUHIRO |
| NEW MATERIAL:A chain terpene compound of formula I [R 1 is H, 1- alkoxyalkyl, tetrapyranyl, tetrahydrofuranyl, acyl, group of formula II (R 3 to R 5 are 1-10C alkyl, phenyl); R 2 is group of formula III or IV [R 6 is H,CO 2R 7; R 7 is 1-4C alkyl; but when R 1 is H, R 6 is not H, and when R 1 is 1-ethoxyethyl, R 7 is not CH 3; R 8 is-C≡CH,-CH=CH 2)]. EXAMPLE: Ethyl 2-acetyl-5,6-dimethyl-10-(2-tetrahydropyranyl)oxy-4, 8-decadienate. USE: An intermediate enabling to profitably prepare Sarcophytol A having an anti-carcinogenesis-promoting action and an antitumor action. PREPARATION: The hydroxyl group of a compound of formula K is protected and the prepared compound of formula L is subjected to a nucleophilic substitu tion reaction with acetoacetic acid ester alkali metal salt to provide the com pound of formula Ia. COPYRIGHT: (C)1992,JPO&Japio | ||||||
| 199 | Chain terpene compound | JP18171089 | 1989-07-14 | JPH0348633A | 1991-03-01 | TAKAYANAGI HISAO; KITANO YASUNORI; MORINAKA YASUHIRO |
| NEW MATERIAL:The compound of formula [R is CO 2R 1 (R 1 is 1-4C alkyl or phenyl), hydroxymethyl, formyl or C(CN)HOR 2 (R 2 is H, trimethylsilyl or 1- ethoxyethyl); X is halogen or OSO 2R 3 (R 3 is (substituted) 1-4C alkyl or phenyl); the double bonds 8, 12 among the double bonds 2, 4, 8, 12 take type-E steric configuration and the double bonds 2, 4 take type-(Z, E), (E, E), (Z, Z) or (E, Z) configuration]. EXAMPLE: 14-Chloro-2-(1-methylethyl)-5,9,13-trimethyl 2,4,1,12-tetradecatetraenoic acid ethyl ester. USE: An intermediate for the total synthesis of an antitumor agent sarcophytol A. PREPARATION: The compound of formula wherein R is CO 2R 1 can be produced e.g. by halogenating a 14-hydroxy-2-(1-methylethyl)-5,9,13-trimethyl 2,4,8,12- tetradecatetraenoic acid ester. COPYRIGHT: (C)1991,JPO&Japio | ||||||
| 200 | 스트랩토키나제와 스트렙토도르나제를 생산하는 미생물 및 이를 이용한 스트렙토키나제 및 스트렙토도르나제의 제조방법 | KR1019940020022 | 1994-08-13 | KR1019960007770A | 1996-03-22 | 최덕호; 배신규; 최미영; 이상훈; 조연덕 |
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