DETECTION OF CHROMOSOME COPY NUMBER CHANGES TO DISTINGUISH MELANOCYTIC NEVI FROM MALIGNANT MELANOMA专利检索- 本小类其它组都不包括的细目专利检索查询-专利查询网

DETECTION OF CHROMOSOME COPY NUMBER CHANGES TO DISTINGUISH MELANOCYTIC NEVI FROM MALIGNANT MELANOMA
申请号	PCT/US0009609	申请日	2000-04-10	公开(公告)号	WO0061814A8	公开(公告)日	2001-05-25
申请人	UNIV CALIFORNIA;			发明人	BASTIAN BORIS; PINKEL DANIEL;
摘要	The present invention provides for methods of distinguishing melanocytic nevi, such as Spitz nevi, from malignant melanoma. The methods comprise contacting a nucleic acid sample from a patient with a probe which binds selectively to a target polynucleotide sequence on a chromosomal region such as 11p, which is usually amplified in Spitz nevi. The nucleic acid sample is typically from skin tumor cells located within a tumor lesion on the skin of the patient. Using another probe which binds selectively to a chromosomal region such as 1q, 6p, 7p, 9p, or 10q, which usually show altered copy number in melanoma, the method can determine that those tumor cells with no changes in copy number of 1q, 6p, 7p, 9p, or 10q, are not melanoma cells but rather Spitz nevus cells. The finding of amplifications of chromosome 11p, particularly the presence of an 11p isochromosome would be an additional indication of Spitz nevus. An increase in copy number of chromosome 11p can also be determined by detecting the presence of an amplification of the H-RAS gene. The amplified gene can be normal or can be a mutated H-RAS gene.
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