Agent and method for determination of calcium

申请号 US3798000D 申请日 1972-08-14 公开(公告)号 US3798000A 公开(公告)日 1974-03-19
申请人 MERCK PATENT GMBH; 发明人 HELGER R;
摘要 An agent for the colorimetric determination of calcium, containing a color reagent, which forms complexes in the presence of calcium ions, and a buffer system which consists essentially of a mixture of amidosulfonic acid, disodium tetraborate and alkali carbonate.
权利要求
  • 2. An agent in accordance with claim 1 wherein said color reagent and amidosulfonic acid are in tablet form and said alkali carbonate and disodium tetraborate are in solution form.
  • 3. An agent in accordance with claim 2, wherein said color reagent comprises phthalein purple and 8-hydroxyquinoline sulfate.
  • 4. An agent in accordance with claim 1 wherein said buffer system comprises in grams per liter of aqueous solution, 4 - 5 g. amidosulfonic acid, 0.5 - 0.8 g. disodium tetraborate, and 8-12 g. sodium carbonate.
  • 5. An agent in accordance with claim 1 wherein said color reagent comprises phthalein purple and 8-hydroxyquinoline sulfate.
  • 6. A method for colorimetric determination of calcium ions in a test solution, comprising mixing said calcium ion-containing test solution with an agent solution comprising a color reagent which forms color complexes in the presence of calcium ions, and a buffer system consisting essentially of amidosulfonic acid, disodium tetraborate, and an alkali carbonate, and thereafter measuring the light absorption of the color complex formed in the solution.
  • 7. A method in accordance with claim 6 wherein said color reagent and said amidosulfonic acid in tablet form are mixed with an aqueous solution of sodium tetraborate and sodium carbonate to form said agent solution.
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