Agent and method for determination of calcium |
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申请号 | US3798000D | 申请日 | 1972-08-14 | 公开(公告)号 | US3798000A | 公开(公告)日 | 1974-03-19 |
申请人 | MERCK PATENT GMBH; | 发明人 | HELGER R; | ||||
摘要 | An agent for the colorimetric determination of calcium, containing a color reagent, which forms complexes in the presence of calcium ions, and a buffer system which consists essentially of a mixture of amidosulfonic acid, disodium tetraborate and alkali carbonate. | ||||||
权利要求 | 2. An agent in accordance with claim 1 wherein said color reagent and amidosulfonic acid are in tablet form and said alkali carbonate and disodium tetraborate are in solution form. 3. An agent in accordance with claim 2, wherein said color reagent comprises phthalein purple and 8-hydroxyquinoline sulfate. 4. An agent in accordance with claim 1 wherein said buffer system comprises in grams per liter of aqueous solution, 4 - 5 g. amidosulfonic acid, 0.5 - 0.8 g. disodium tetraborate, and 8-12 g. sodium carbonate. 5. An agent in accordance with claim 1 wherein said color reagent comprises phthalein purple and 8-hydroxyquinoline sulfate. 6. A method for colorimetric determination of calcium ions in a test solution, comprising mixing said calcium ion-containing test solution with an agent solution comprising a color reagent which forms color complexes in the presence of calcium ions, and a buffer system consisting essentially of amidosulfonic acid, disodium tetraborate, and an alkali carbonate, and thereafter measuring the light absorption of the color complex formed in the solution. 7. A method in accordance with claim 6 wherein said color reagent and said amidosulfonic acid in tablet form are mixed with an aqueous solution of sodium tetraborate and sodium carbonate to form said agent solution. |
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