Process for producing a protein hydrolysate
阅读：149发布：2022-09-26
专利汇可以提供Process for producing a protein hydrolysate专利检索，专利查询，专利分析的服务。并且A protein hydrolysate having a high glutamic acid content is produced by adding a glutaminase produced by such microorganisms as bacteria, yeasts, ray fungus, etc. to protein-containing materials when the protein is hydrolysed by a proteolytic enzyme and hydrolyzing the protein thereby to prevent glutamine liberated by proteolysis from conversion to pyroglutamic acid.，下面是Process for producing a protein hydrolysate专利的具体信息内容。
1. A PROCESS FOR PRODUCING A PROTEIN HYDROLYSATE HAVING A HIGH GLUTAMIC ACID CONTENT, WHICH COMPRISES ADDING A GLUTAMINASE TOGETHER WITH A PROTEOLYTIC ENZYME TO A SALT FREE DENATURED PROTEIN-CONTAINING RAW MATERIAL AND HYDROLYZING IN A CLOSED SYSTEM THE PROTEIN CONTAINED IN THE RAW MATERIAL.
2. A process according to claim 1, wherein the glutaminase is a salt-resistant glutaminase.
3. A process according to claim 1, wherein the glutaminase is a heat-resistant glutaminase.
4. A process according to claim 1, wherein the proteolytic enzyme is a crude enzyme or purified enzyme of animal, vegetable or microorganism origin, or a culture of the microorganism produced by using a culture medium free of protein-containing raw material.
5. A process according to claim 1, wherein the hydrolysis is carried out at 30*-70*C.
6. A process according to claim 1, wherein the hydrolysis is carried out at a pH of 2.5-9.0.
7. A process for producing a protein hydrolysate having a high glutamic acid content, which comprises adding to a salt free denatured protein-containing raw material, cells, dried cells, filtrate of culture, crude enzyme, purified enzyme or a crude enzyme-containing solution containing glutaminase which is produced by culturing at least one microorganism capable of producing glutaminase selected from Escherichia coli B IAM 1268 (ATCC 11303), Bacillus subtilis, Bacillus coagulans, Bacillus megaterium NRRL B-939, Proteus vulgaris IAM 1025, Pseudomonas aeruginosa, Pseudomonas ovalis R-962, Pseudomonas ovalis 3-5, Pseudomonas fluorescens IAM 1179, Pseudomonas reptilivora S 1105, Pseudomonas schuylkilliensis IAM 1154 (NRRL B-6), Clostidium perfringes, Staphilococcus aureus, Achromobacter liquefaciens TR-9, Bacterium succinicum IAM 1017, Serratia marcescens IAM 1184, Cryptococcus laurenti, Cryptococcus albidus ATCC 20293, Cryptococcus albidus ATCC 20294, Saccharomyces cerevisiae, Saccharomyces rouxii ATCC 13356, Candida utilis, Streptomyces griseus ATCC 10137, Streptomyces fradiae, Streptomyces globisporus, Aspergillus oryzae, Aspergillus sojae IAM 2665, Penicillium chrysogenum, Rhizopus oryzae and Aspergillus awamori ATCC 14335, together with a proteolytic enzyme, and hydrolyzing in a closed system the protein contained in the raw material.
8. A process according to claim 7, wherein the hydrolysis is carried out at 30*-70*C.
9. A process according to claim 7, wherein the hydrolysis is carried out at a pH of 2.5-9.0.
10. A process for producing a protein hydrolysate having a high glutamic acid content, which comprises adding a heat-resistant glutaminase together with a proteolytic enzyme to a protein-containing raw material, or adding a heat-resistant glutaminase to a protein-containing denatured raw material in which a proteolytic enzyme is present and hydrolyzing in a closed system the protein contained in the raw material at a temperature of at least 50*C.
11. A process according to claim 10, wherein the heat-resistant glutaminase is one produced by culturing at least one microorganism capable of producing a heat-resistant glutaminase selected from the group consisting of Bacillus megaterium NRRL B-939, Pseudomonas ovalis 3-5, Pseudomonas reptilivora S 1105, Serratia marcescens IAM 1184, Achromobacter liquefaciens TR-9, Bacterium succinicum IAM 1017, Cryptococcus albidus K-0434, ATCC 20294.
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