| 序号 | 专利名 | 申请号 | 申请日 | 公开（公告）号 | 公开（公告）日 | 发明人 |
|---|---|---|---|---|---|---|
| 1 | 葡萄扇叶病毒的RT-LAMP检测方法 | CN202310151477.9 | 2023-02-22 | CN116397053A | 2023-07-07 | 谈静惠; 陈林; 谢婷; 张博文 |
| 本发明提供了葡萄扇叶病毒的实时环介导等温扩增(RT‑LAMP)检测方法，所述方法是以葡萄扇叶病毒的外壳蛋白基因为目标基因设计引物，利用引物进行实时环介导等温扩增检测技术检测鉴定葡萄扇叶病毒。本发明提供的检测方法特异性强，灵敏度高，鉴定结果直观，试验操作简单，仪器成本低。 | ||||||
| 2 | Grapevine fanleaf virus resistance in grapevine expressing grapevine fanleaf virus coat protein | US09162017 | 1998-09-28 | US06667426B1 | 2003-12-23 | Dennis Gonsalves; Baodi Xue; Tania Krastanova; Kai-Shu Ling; Marc Fuchs |
| In general, the invention features a method for selecting a transgenic grapevine or grapevine component having increased resistance to a fanleaf disease, the method including the steps of: (a) transforming a grape plant cell with a grape nepovirus coat protein nucleic acid molecule or fragment thereof which is capable of being expressed in the plant cell; (b) regenerating a transgenic grapevine or grapevine component from the plant cell; and (c) selecting a transgenic grapevine or grapevine component which expresses, at a low level, the nucleic acid molecule or fragment thereof, wherein the low level expression increases the resistance of the transgenic grapevine or grapevine component to fanleaf disease. The invention also relates to an isolated nucleic acid molecule encoding a coat protein or fragment thereof of a ‘Geneva’ isolate of a grapevine nepovirus virus. | ||||||
| 3 | Engineering broad and durable resistance to grapevine fanleaf virus in plants | US13126956 | 2009-11-02 | US08669419B2 | 2014-03-11 | Marc Fuchs; Jonathan Oliver |
| The present invention relates to nucleic acid molecules useful for conferring broad and durable resistance to grapevine fanleaf virus in plants. The invention also relates to methods of enhancing resistance to plant pathogens and plants or plant components (such as grape plants) expressing such nucleic acid molecules. In addition, the invention relates to products (e.g., foodstuffs including beverages such as wine or juice) derived from grape plants transformed with such nucleic acids. | ||||||
| 4 | 葡萄树中的线虫传多面体病毒抗性 | CN98811600.6 | 1998-09-29 | CN1280457A | 2001-01-17 | D·戈沙维斯; B·薛; T·克拉斯塔诺瓦; K·S·林 |
| 一般来说,本发明的特征在于选择具有增强的对扇叶病抗性的转基因葡萄树或葡萄树部分的一种方法,该方法包括以下步骤(a)用能在植物细胞中表达的葡萄线虫传多面体病毒外壳蛋白核酸分子或其片段转化葡萄植物细胞;(b)从该植物细胞再生转基因葡萄树或葡萄树部分;和(c)选择低水平表达核酸分子或其片段的转基因葡萄树或葡萄树部分,其中低水平表达增强转基因葡萄树或葡萄树部分对扇叶病的抗性。本发明还涉及编码葡萄树线虫传多面体病毒的日内瓦分离株的外壳蛋白或其片段的分离的核酸分子。 | ||||||
| 5 | 杀菌灭病毒净化型空气过滤装置 | CN200320112070.3 | 2003-10-30 | CN2655088Y | 2004-11-10 | 罗雁 |
| 一种杀菌灭病毒净化型空气过滤装置，其特征在于：在中央空调的管道中有扇叶架，扇叶架上安装有垂直于中央空调的管道轴向的用高强度轻型耐腐蚀材料制成的，表面是网状透孔的，杀菌灭病毒净化型空气扇叶，杀菌灭病毒净化型空气扇叶中间装有杀菌灭病毒剂。每扇杀菌灭病毒净化型空气扇叶内是几个独立的空间，各个独立的空间互不连通，每扇杀菌灭病毒净化型空气扇叶各个独立的空间内装有杀菌灭病毒剂。其特点是：能100％的杀灭SARS－冠状病毒、甲3型流感病毒、呼吸道合胞病毒、腺病毒及大肠杆菌、金黄色葡萄球菌、肺炎克雷伯氏菌、枯草芽孢杆菌、黑曲霉菌并能去除甲醛、苯等有毒、有害气体及异臭、异味达到空气消毒净化的效果。 | ||||||
| 6 | 一种葡萄试管内集成脱毒方法 | CN201310074449.8 | 2013-03-10 | CN103109744A | 2013-05-22 | 顾地周; 顾川岳; 陆爽; 姜云天; 朱俊义; 杨丽娟; 潘雨; 禚玲玲; 张学士; 王秋爽; 付航; 倪伟佳 |
| 本发明涉及一种葡萄试管内集成脱毒方法，属于植物繁殖技术。其步骤如下：（1）将葡萄休眠枝条水培促使休眠芽萌发生长，处理后的茎尖作为外植体备。（2）将切取的葡萄茎尖接种至附加吲哚丁酸0.08～0.13mg·L-1和赤霉素2.20～3.30mg·L-1，添加食用白糖5g·L-1，琼脂条8.0g·L-1的基本培养基中进行脱除葡萄扇叶病毒和卷叶病毒培养；（3）培养温度控制在45～48℃，培养时间51～57d。（4）当茎尖生长至1.0cm时，即可切割0.03～0.05cm的茎尖进一步开展无毒苗培养。葡萄扇叶病毒和卷叶病毒脱除率达100.0%；无毒苗成活率达97.0%以上。可直接应用于葡萄品种脱毒种苗的工厂化生产。 | ||||||
| 7 | 一种葡萄试管内集成脱毒方法 | CN201310074449.8 | 2013-03-10 | CN103109744B | 2014-05-14 | 顾地周; 顾川岳; 陆爽; 姜云天; 朱俊义; 杨丽娟; 潘雨; 禚玲玲; 张学士; 王秋爽; 付航; 倪伟佳 |
| 本发明涉及一种葡萄试管内集成脱毒方法，属于植物繁殖技术。其步骤如下：（1）将葡萄休眠枝条水培促使休眠芽萌发生长，处理后的茎尖作为外植体备。（2）将切取的葡萄茎尖接种至附加吲哚丁酸0.08～0.13mg·L-1和赤霉素2.20～3.30mg·L-1，添加食用白糖5g·L-1，琼脂条8.0g·L-1的基本培养基中进行脱除葡萄扇叶病毒和卷叶病毒培养；（3）培养温度控制在45～48℃，培养时间51～57d。（4）当茎尖生长至1.0cm时，即可切割0.03～0.05cm的茎尖进一步开展无毒苗培养。葡萄扇叶病毒和卷叶病毒脱除率达100.0%；无毒苗成活率达97.0%以上。可直接应用于葡萄品种脱毒种苗的工厂化生产。 | ||||||
| 8 | 一种防治犬细小病毒的复方水剂及其制备方法 | CN201410732055.1 | 2014-12-06 | CN104667236A | 2015-06-03 | 阎华; 王会; 黄升谋 |
| 一种治疗犬细小病毒病的复方水剂及其制备方法，涉及治疗动物医药领域。本发明按重量称取菊花粉、淡竹叶粉，艾叶粉，鱼腥草粉，三七粉，绿茶粉，泽泻粉，大黄粉，葛根粉，大蒜粉，生姜粉。地塞米松，葡萄糖，维生素C，叶酸，碳酸钙。鱿鱼干粉，扇贝粉。经过处理后合并组合物，即可获得防治犬细小病毒复方水剂。本发明的有益效果是：组方合理，制备简单，该组合物无毒副作用、无污染、无药物残留、无耐药性，生物利用度高，疗效非常显著，临床证明对治疗犬细小病毒病有特效。 | ||||||
| 9 | NEPOVIRUS RESISTANCE IN GRAPEVINE | PCT/US9820272 | 1998-09-29 | WO9916298A9 | 1999-07-15 | GONSALVES DENNIS; XUE BAODI; KRASTANOVA TANIA; LING KAI-SHU |
| In general, the invention features a method for selecting a transgenic grapevine or grapevine component having increased resistance to a fanleaf disease, the method including the steps of: (a) trnsforming a grape plant cell with a grape nepovirus coat protein nucleic acid molecule or fragment thereof which is capable of being expressed in the plant cell; (b) regenerating a transgenic grapevine or grapevine component from the plant cell; and (c) selecting a transgenic grapevine or grapevine component which expresses, at a low level, the nucleic acid molecule or fragment thereof, wherein the low level expression increases the resistance of the transgenic grapevine or grapevine component to fanleaf disease. The invention also relates to an isolated nucleic acid molecule encoding a coat protein or fragment thereof of a "Geneva" isolate of a grapevine nepovirus virus. | ||||||
| 10 | 포도부채잎바이러스 피시알 검사 시스템 | KR1020090003073 | 2009-01-14 | KR1020100083607A | 2010-07-22 | 이수헌; 신용길; 한상진; 최홍수; 김정수; 김현란 |
| PURPOSE: A PCR test system for diagnosing GFLV(grapevine fanleaf virus) is provided to diagnose and to easily diagnose. CONSTITUTION: A primer for diagnosing grapevine fanleaf virus is primer complex sequence numbers 8 and 19, sequence numbers 9 and 18, sequence number 11 and 16, and sequence number 12 and 15. The diagnosis is performed by RT-PCR using the primer with a genome RNA as a template. | ||||||
| 11 | GENERATION OF GRAPEVINE ROOTSTOCKS THAT PROVIDE RESISTANCE AND SANITATION IN RELATION TO GRAPEVINE FANLEAF VIRUS (GFLV) | PCT/IB2012056297 | 2012-11-09 | WO2013072827A3 | 2013-08-08 | ARCE JOHNSON PATRICIO; TORRES PACHECO ELIZABETH; GODOY SANTIN FRANCISCA; MEDINA AREVALO CONSUELO; CADAVID LABRADA AGNES; VALENZUELA VALDES PABLO; BRUNO URBINA CONSUELO |
| The invention relates to a vector plasmid known as "GFLV silencing construct" which provides resistance and sanitation in relation to grapevine fanleaf virus (GFLV), to plant cells transformed with said vector plasmid and to a method for conferring resistance and sanitation in relation to grapevine fanleaf virus (GFLV) to non-transgenic grapevines by grafting same on seedlings generated from the cells transformed with said vector plasmid. The "GFLV silencing construct" vector plasmid of the invention contains inverted duplicates of sequences that code for the capsid protein of grapevine fanleaf virus (GFLV). | ||||||
| 12 | ENGINEERING BROAD AND DURABLE RESISTANCE TO GRAPEVINE FANLEAF VIRUS IN PLANTS | PCT/US2009/063013 | 2009-11-02 | WO2010051548A2 | 2010-05-06 | FUCHS, Marc; OLIVER, Jonathan |
The present invention relates to nucleic acid molecules useful for conferring broad and durable resistance to grapevine fanleaf virus in plants. The invention also relates to methods of enhancing resistance to plant pathogens and plants or plant components (such as grape plants) expressing such nucleic acid molecules. In addition, the invention relates to products (e.g., foodstuffs including beverages such as wine or juice) derived from grape plants transformed with such nucleic acids. |
||||||
| 13 | ENGINEERING BROAD AND DURABLE RESISTANCE TO GRAPEVINE FANLEAF VIRUS IN PLANTS | PCT/US2009063013 | 2009-11-02 | WO2010051548A3 | 2010-08-05 | FUCHS MARC; OLIVER JONATHAN |
| The present invention relates to nucleic acid molecules useful for conferring broad and durable resistance to grapevine fanleaf virus in plants. The invention also relates to methods of enhancing resistance to plant pathogens and plants or plant components (such as grape plants) expressing such nucleic acid molecules. In addition, the invention relates to products (e.g., foodstuffs including beverages such as wine or juice) derived from grape plants transformed with such nucleic acids. | ||||||
| 14 | GENERACIÓN DE PORTAINJERTOS DE VID PARA RESISTENCIA Y SANEAMIENTO AL VIRUS DE LA HOJA EN ABANICO DE LA VID (GFLV) | PCT/IB2012/056297 | 2012-11-09 | WO2013072827A2 | 2013-05-23 | ARCE JOHNSON, Patricio; TORRES PACHECO, Elizabeth; GODOY SANTIN, Francisca; MEDINA AREVALO, Consuelo; CADAVID LABRADA, Agnes; VALENZUELA VALDES, Pablo; BRUNO URBINA, Consuelo |
La invención divulga un plásmido vector llamado "GFLV silencing construct" que confiere resistencia y saneamiento al virus de la hoja en abanico de la vid (GFLV); células vegetales transformadas con dicho plásmido vector y un método para conferir resistencia y saneamiento al virus de la hoja en abanico de la vid (GFLV) en vides no transgénicas al injertarlas en plántulas generadas desde las células transformadas con dicho plásmido vector. El plásmido vector de la invención "GFLV silencing construct" contiene duplicados invertidos de secuencias que codifican para la proteína de la cápside del virus de la hoja en abanico de la vid (GFLV). |
||||||
| 15 | GENERATION OF GRAPEVINE ROOTSTOCKS THAT PROVIDE RESISTANCE AND SANITATION IN RELATION TO GRAPEVINE FANLEAF VIRUS (GFLV) | US14358884 | 2012-11-09 | US20150067916A1 | 2015-03-05 | Patricio Arce Johnson; Elizabeth Torres Pacheco; Francisca Godoy Santin; Consuelo Medina Arevalo; Agnes Cadavid Labrada; Pablo Valenzuela Valdes; Consuelo Bruno Urbina |
| The invention discloses a vector plasmid called “GFLV silencing construct” that confers resistance and sanitation against the grapevine fanleaf virus (GFLV); plant cells transformed with said vector plasmid and a method to impart resistance and sanitation against the grapevine fanleaf virus (GFLV) in non-transgenic grapevines when being grafted onto seedlings generated from cells transformed with said plasmid vector. The “GFLV silencing construct” plasmid vector of the invention comprises inverted sequence duplicates coding for the grapevine fanleaf virus (GFLV) capsid protein. | ||||||
| 16 | Grapevine fanleaf virus resistance in grapevine | US10377435 | 2003-02-28 | US07211710B2 | 2007-05-01 | Dennis Gonsalves; Baodi Xue; Tania Krastanova; Kai-Shu Ling; Marc Fuchs |
| In general, the invention features a method for selecting a transgenic grapevine or grapevine component having increased resistance to a grapevine fanleaf disease. The method involves (a) transforming a grape plant cell with a grapevine fanleaf virus coat protein nucleic acid molecule or fragment thereof which is expressed in the plant cell; (b) regenerating a transgenic grapevine or grapevine component from the plant cell; and (c) selecting a transgenic grapevine or grapevine component expressing a grapevine fanleaf virus coat protein or coat protein fragment thereof, where the coat protein or the coat protein fragment thereof is expressed at a level equal to or less than an optical density of about 0.2 at 405 nm as detected by an enzyme-linked immunoassay as compared to a control plant, the selected transgenic grapevine or grapevine component having resistance to the grapevine fanleaf disease. | ||||||
| 17 | Grapevine fanleaf virus resistance in grapevine | US10377435 | 2003-02-28 | US20030226172A1 | 2003-12-04 | Dennis Gonsalves; Baodi Xue; Tania Krastanova; Kai-Shu Ling; Marc Fuchs |
| In general, the invention features a method for selecting a transgenic grapevine or grapevine component having increased resistance to a grapevine fanleaf disease. The method involves (a) transforming a grape plant cell with a grapevine fanleaf virus coat protein nucleic acid molecule or fragment thereof which is expressed in the plant cell; (b) regenerating a transgenic grapevine or grapevine component from the plant cell; and (c) selecting a transgenic grapevine or grapevine component expressing a grapevine fanleaf virus coat protein or coat protein fragment thereof, where the coat protein or the coat protein fragment thereof is expressed at a level equal to or less than an optical density of about 0.2 at 405 nm as detected by an enzyme-linked immunoassay as compared to a control plant, the selected transgenic grapevine or grapevine component having resistance to the grapevine fanleaf disease. | ||||||
| 18 | NEPOVIRUS RESISTANCE IN GRAPEVINE | EP98951963 | 1998-09-29 | EP1026941A4 | 2004-05-26 | GONSALVES DENNIS; XUE BAODI; KRASTANOVA TANIA; LING KAI-SHU |
| In general, the invention features a method for selecting a transgenic grapevine or grapevine component having increased resistance to a fanleaf disease, the method including the steps of: (a) transforming a grape plant cell with a grape nepovirus coat protein nucleic acid molecule or fragment thereof which is capable of being expressed in the plant cell; (b) regenerating a transgenic grapevine or grapevine component from the plant cell; and (c) selecting a transgenic grapevine or grapevine component which expresses, at a low level, the nucleic acid molecule or fragment thereof, wherein the low level expression increases the resistance of the transgenic grapevine or grapevine component to fanleaf disease. The invention also relates to an isolated nucleic acid molecule encoding a coat protein or fragment thereof of a 'Geneva' isolate of a grapevine nepovirus virus. | ||||||
| 19 | NEPOVIRUS RESISTANCE IN GRAPEVINE | EP98951963.2 | 1998-09-29 | EP1026941A1 | 2000-08-16 | GONSALVES, Dennis; XUE, Baodi; KRASTANOVA, Tania; LING, Kai-Shu |
| In general, the invention features a method for selecting a transgenic grapevine or grapevine component having increased resistance to a fanleaf disease, the method including the steps of: (a) transforming a grape plant cell with a grape nepovirus coat protein nucleic acid molecule or fragment thereof which is capable of being expressed in the plant cell; (b) regenerating a transgenic grapevine or grapevine component from the plant cell; and (c) selecting a transgenic grapevine or grapevine component which expresses, at a low level, the nucleic acid molecule or fragment thereof, wherein the low level expression increases the resistance of the transgenic grapevine or grapevine component to fanleaf disease. The invention also relates to an isolated nucleic acid molecule encoding a coat protein or fragment thereof of a 'Geneva' isolate of a grapevine nepovirus virus. | ||||||
| 20 | Hybrid grapevine rootstock named 'VR 043-43' | US86653786 | 1986-05-23 | US6319P | 1988-10-04 | LIDER LLOYD A; OLMO HAROLD P; GOHEEN AUSTIN C |
| A new and distinct variety of grapevine rootstock is provided. It is characterized by its immunity to feeding by the nematode vector of grapevine fanleaf virus, and when grafted with cultivars, such as 'Cabernet Sauvignon,' 'Chenin blanc,' and 'Carignane,' may be planted in soil contaminated with viruliferous nematodes, producing healthy fruit and excellent yields. | ||||||
QQ群二维码
意见反馈
意见反馈