The group of inventions relates to chemical/pharmaceutical industry and medicine and concerns production of biologically active substances, in particular, a protein/polypeptide complex, method for producing the same, and the claimed complex-based pharmcomposition. This complex is produced by consecutive treatment of fetal nerve and skin tissues of hoofed farm animals, using filtration, centrifugation, and anion-exchange chromatography with an eluent buffer having a defined ionic strength. This complex includes negatively charged faintly acid and neutral proteins and polypeptides with molecular weights ranging from 5 to 200 kDa, therewith at least 70% of the total protein amount having molecular weights ranging from 20 to 160 kDa. The complex is characterized with a set of specific bands at denaturing gel electrophoresis («SDS-Page») in 5% polyacrylamide gel.

The complex makes antihypoxic, tissue-specific reparative and rejuvenating effect on skin tissue. It is used as an active ingredient in pharmaceutical compositions for producing therapeutic and cosmetic agents.

The pharmcomposition includes the protein/polypeptide complex and excipients - preservatives, detergents, solubilizers etc. The pharmcomposition makes improved antihypoxic, tissue-specific reparative and rejuvenating effect on skin tissue. The pharmcomposition is used as a preparation intended to treat various skin diseases (vascular, autoimmune, traumatic, toxic) in aesthetic medicine, gerontology and beauty therapy.

The invention refers to medicine, in particular to oncology, pharmacology and veterinary science, and can be used for treatment and prevention of neoplastic processes of skin tissues - squamous-cell and basal-cell skin cancer, as well as melanoma and other benignant and malignant skin tumors, with the help of pharmaceutical preparation including protein-polypeptide complex produced from homogenates of nervous and skin tissues of the hoofed livestock.

In the claimed invention, protein-polypeptide complex is used as a primary active substance (biologically active substance), it is represented as a biologically active protein-polypeptide complex (hereinafter - PPC) with the molecular mass of its constituent components within the range of 5-200 kDa and content of medium molecular fraction within the range of 10 to 120 kDa in at least 80%, general protein content 0.2 - 4.2 mg/ml, maximum absorption peak of UV spectrum of solution at the wave length 280±5nm, peak presence at the wave length 274-284 nm in the UV-visible spectrum, presence of specific bands within pl range from 4.2 to 8.4 at isoelectric focusing in 5% polyacrilamide gel and pharmaceutically acceptable agent, for example diluents, as well as conventional agents (pharmaceutically acceptable) upon composition of pastes, gels, creams, etc., and the diluents can include buffer solution and excipients - high-molecular compounds belonging to the class of preservatives and solubilizers in sufficient concentrations.

The substance is produced by ion-exchange chromatography of filtrate of extracted homogenate of skin, cerebral and spinal cord tissues of hoofed livestock's embryos with the gestation period from the middle of the first third till the middle of the last third in the presence of buffer solution, detergents, proteolysis inhibitors and solubilizers.

The method implies use of the specified protein-polypeptide complex both in the form of solutions, suspension in pharmaceutically acceptable carriers, diluents in composition of auxiliary additives, and in the form of pastes, gels, liposomal emulsion, both for treatment and for simultaneous treatment and prevention of oncological skin diseases.