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Soybean fractionation employing a protease

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专利汇可以提供Soybean fractionation employing a protease专利检索,专利查询,专利分析的服务。并且Process of separating nutritional components from soybeans or other oilseeds, employing enzymatic hydrolysis of protein. Clean, whole soybeans are comminuted, heated at about 90*-140* C., and then cooled by adding more water. A proteolytic enzyme, preferably derived from an Aspergillus or Bacillus sp., is added to the slurry, which is then maintained at an incubation temperature of 25*-75* C. until the protein is sufficiently hydrolyzed. The fibrous solids portion is then removed from the slurry, being useful as a livestock feed supplement. The oil phase may then be removed from the fluid portion of the slurry, as by separating an aqueous phase from an oil-containing phase in a cream separator. The aqueous phase is then acidified to about pH 4.5, to precipitate isoelectric protein, which may be recovered, e.g., by centrifugation. To obtain the main product of this process, soy protein hydrolysate, the remaining aqueous phase is desirably concentrated by conventional evaporation techniques, adjusted to substantially neutral pH, and then dried, e.g., by spray drying or lyophilization. Alternatively, the aqueous phase containing protein and/or protein hydrolysate may be employed as the basis of a nutritious beverage.,下面是Soybean fractionation employing a protease专利的具体信息内容。

  • 2. The process of claim 1, wherein said comminuted oilseeds are heated at a temperature between about 90* and 140* C.
  • 3. The process of claim 1, wherein said incubation slurry has a temperature between about 25* and 75* C. and a pH between about 5 and 9.5.
  • 4. The process of claim 1, wherein said enzyme composition comprises a proteolytic enzyme derived from a fungus, bacterium, or mixture thereof.
  • 5. The process of claim 4, wherein said fungus is an Aspergillus and said bacterium is a Bacillus.
  • 6. The process of claim 4, wherein said enzyme composition further comprises a carbohydrase, lipase or cellulase.
  • 7. The process of claim 1, wherein said fluid product comprises an oil phase consisting essentially of crude oilseed oil and further comprises the rest of said fluid product consisting essentially of a protein-containing, predominately aqueous, fluid mixture; further comprising the step of: separating at least a portion of the oil phase from the rest of said fluid product to recover crude oilseed oil and a protein-containing, predominately aqueous fluid mixture.
  • 8. The process of claim 1, wherein the oilseeds are soybeans.
  • 9. The process of claim 8, wherein said comminuted oilseeds comprise soybeans ground to particles between about 10 and 100 mesh.
  • 10. The process of claim 8, wherein said fluid product comprises an oil phase consisting essentially of crude oilseed oil and further comprises the rest of said fluid product consisting essentially of a protein-containing, predominately aqueous fluid mixture; further comprising the steps of: separating at least a portion of the oil phase from the rest of said fluid product to recover crude soybean oil and a protein-containing, predominately aqueous fluid mixture.
  • 11. The process of claim 10, wherein the oil phase is separated from the aqueous phase of said fluid product to recover crude soybean oil and a protein-containing aqueous phase.
  • 12. The process of claim 11, further comprising the steps of: adjusting the pH of said aqueous phase to the isoelectric point of the protein therein; and separating the isoelectric precipitate from the remaining solution.
  • 13. The process of claim 12, further comprising the step of: drying the remaining solution to recover soy protein hydrolysate.
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