Method for producing secondary metabolites of plants
专利汇可以提供Method for producing secondary metabolites of plants专利检索，专利查询，专利分析的服务。并且This invention provides for a method for producing secondary metabolites of higher plants, such as Lithospermum erythrorhizon, Coptis japonica, and Nicotinia tabacum, by suspension cultures of a mass of undifferentiated cells (callus). The cultures are carried out in at least two stages in liquid media. The first stage culturing is carried out in a liquid medium conventionally used for the tissue culture of plants, which contains indispensably inorganic substances and carbon sources and, additionally, phytohormones, vitamins and/ or amino acids. While the second stage culturing is carried out in another liquid medium, of which the concentration of at least one of the constituents is substantially varied, namely decreased or increased, from the first stage liquid medium. According to such two stage liquid medium cultures, the productivity of the second metabolites, such as shikonin, berberine and nicotine, and significantly increased, even though the rate of cell growth in the second stage is relatively low.，下面是Method for producing secondary metabolites of plants专利的具体信息内容。
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