BACKGROUND OF THE INVENTION

&null;0002&null; Nematodes are important plant pests which cause millions of dollars of damage each year to turf grasses, ornamental plants, and food crops. Efforts to eliminate or minimize damage caused by nematodes in agricultural settings have typically involved the use of soil fumigation with materials such as chloropicrin, methyl bromide, and dazomet, which volatilize to spread the active ingredient throughout the soil. Such fumigation materials can be highly toxic and may create an environmental hazard. Various non-fumigant chemicals have also been used, but these, too, create serious environmental problems and can be highly toxic to humans.

&null;0003&null; The accepted methodology for control of nematodes afflicting animals has centered around the use of the drug benzimidazole and its congeners. The use of these drugs on a wide scale has led to many instances of resistance among nematode populations (Prichard, R. K. et al. &null;1980&null; &null;The problem of anthelmintic resistance in nematodes,&null; Austr. Vet. J. 56:239-251; Coles, G. C. &null;1986&null; &null;Anthelmintic resistance in sheep,&null; In Veterinary Clinics of North America: Food Animal Practice, Vol 2:423-432 &null;Herd, R. P., Eds.&null; W. B. Saunders, New York).

&null;0004&null; The pesticidal activity of avermectins is well known. The avermectins are disaccharide derivatives of pentacyclic, 16-membered lactones. They can be divided into four major compounds: A1a, A2a, B1a, and B2a; and four minor compounds: A1b, A2b, B1b, and B2b.

&null;0005&null; The organism which produces avermectins was isolated and identified as Streptomyces avermitilis MA-4680 (NRRL-8165). Characteristics of the avermectin producing culture and the fermentation process are well documented and known to those skilled in the art (Burg, R. W. et al. &null;1979&null; &null;Avermectins, New Family of Potent Anthelmintic Agents: Producing Organism and Fermentation,&null; Antimicrob. Agents Chemother. 15(3):361-367). The isolation and purification of these compounds is also described in U.S. Pat. No. 4,310,519, issued Jan. 12, 1982.

&null;0006&null; Another family of pesticides produced by fermentation are the milbemycins, which are closely related to the avermectins. The milbemycins can be produced by a variety of Streptomyces and originally differed from the avermectins only in the C-13 position. The milbemycins and their many derivatives are also well known to those skilled in the art and are the subject of U.S. patents. See, for example, U.S. Pat. No. 4,547,520.

&null;0007&null; While the avermectins were initially investigated for their anthelmintic activities, they were later found to have other insecticidal properties, although the degree varies. The activity of avermectins must generally be determined empirically.

&null;0008&null; 22,23-dihydroavermectin B1 is a synthetic derivative of the avermectins and has been assigned the nonproprietary name of ivermectin. It is a mixture of 80% 22,23-dihydroavermectin B1a and 20% 22,23-dihydroavermectin B1b. Ivermectin has been tested on a variety of laboratory and domestic animals for control of nematodes, ticks, and heartworms.

&null;0009&null; Avermectin B2a is active against the root-knot nematode, Meloidogyne incognita. It is reported to be 10-30 times as potent as commercial contact nematicides when incorporated into soil at 0.16-0.25 kg/ha (Boyce Thompson Institute for Plant Research 58th Annual Report &null;1981&null;; Putter, I. et al. &null;1981&null; &null;Avermectins: Novel Insecticides, Acaracides, and Nematicides from a Soil Microorganism,&null; Experientia 37:963-964). Avermectin B2a is not toxic to tomatoes or cucumbers at rates of up to 10 kg/ha. Avermectin B1 is a combination of avermectin B1a (major component) and avermectin B1b. It has demonstrated a broad spectrum of insecticidal activities. The data indicate that avermectin B1 is primarily a miticide, although it is also effective on the Colorado potato beetle, potato tuberworm, beet army worm, diamondback moth, gypsy moth, and the European corn borer.

&null;0010&null; The use of avermectins in various agricultural applications has been described in publications and patents. The use of avermectin with spray oils (lightweight oil compositions) has been described. See, for example, U.S. Pat. No. 4,560,677 issued Dec. 24, 1985; EPO applications0 094 779 and 0 125 155; and Anderson, T. E., J. R. Babu, R. A. Dybas, H. Mehta (1986) J. Econ. Entomol. 79:197-201.

&null;0011&null; There is a continuing need for new, alternative materials and methods useful for killing nematodes.

BRIEF SUMMARY OF THE INVENTION

&null;0012&null; The subject invention concerns compositions and processes for controlling nematodes. In one embodiment, the subject invention comprises the use of certain 3,5-bis-alkylamino-1,2,4 thiadiayol compounds to control nematodes which infest and afflict animals. Nematodes which infest plants or the situs of plants can also be controlled using the methods and compositions of the subject invention, as can other acarid and arthropod pests.

&null;0013&null; Preferred compounds useful according to the subject invention can be represented by the Formulae I, II, III, IV, and V as further described herein.

&null;0014&null; 1. A urea derivative of the following Formula I:

Ar&null;(Alk)0-1&null;NH&null;CO&null;NR1&null;Alk&null;R2&null;&null;(Formula I)

&null;0015&null; wherein Ar is aryl or heteroaryl optionally substituted by one or more R3 groups;

&null;0016&null; each Alk is a linear or cyclic alkylene radical of up to 8 C atoms;

&null;0017&null; R1 is H or C1-6 alkyl;

&null;0018&null; R2 is heteroaryl or heterocycloalkyl optionally substituted by Ar, or forms such a group by cyclisation with R1; and

&null;0019&null; R3 is OH, halogen, CF3, OCF, or a group selected from NH2, SO2&null;C1-6 alkyl, C6-10 aryl,

&null;0020&null; C6-10 aryloaxy, C5-6 cycloalkyl, C1-5 alkoxy, and C1-6alkyl, said group being optionally substituted by OH, C1-6 alkoxy, C1-6 alkyl, phenyl, halogen, or CF3.

&null;0021&null; Particularly preferred anthelmintic compounds according to Formula I are exemplified herein by compounds represented by structures 1-10 (depicted in FIGS. 1-10, respectively), which have been assigned the respective reference numbers:

1

AKC 111

(STRUCTURE 1),

AKC 112

(STRUCTURE 2),

AKC 113

(STRUCTURE 3),

AKC 107

(STRUCTURE 4),

AKC 114

(STRUCTURE 5),

AKC 108

(STRUCTURE 6),

AKC 115

(STRUCTURE 7),

AKC 116

(STRUCTURE 8),

AKC 117

(STRUCTURE 9), and

AKC 118

(STRUCTURE 10).

&null;0022&null; 2. A heterocycle-substituted amide of the following Formula II:

Ar&null;(Alk)0-1&null;NH&null;CO&null;Het&null;&null;(Formula II)

&null;0023&null; wherein Ar is aryl or heteroaryl optionally substituted by one or more R3 groups;

&null;0024&null; each Alk is an optionally cyclic alkylene radical of up to 8 C atoms;

&null;0025&null; Het is heteroaryl or heterocycloalkyl optionally substituted by Ar and/or R3; and

&null;0026&null; R3 is OH, halogen, CF3, OCF3, or a group selected from NH2, SO2 alkyl, C6-10 aryl, C1-6 alkoxy, and C1-6 alkyl, said group being optionally substituted by OH, C1-6 alkoxy, C1-6 alkyl, phenyl, halogen, or CF3.

&null;0027&null; Particularly preferred anthelmintic compounds according to Formula II are exemplified herein by compounds represented by structures 11-25 (depicted in FIGS. 11-25 respectively), which have been assigned the respective reference numbers:

2

ARC 119

(STRUCTURE 11),

AKC 110

(STRUCTURE 12),

ARC 120

(STRUCTURE 13),

ARC 121

(STRUCTURE 14),

ARC 2153

(STRUCTURE 15),

AKC 122

(STRUCTURE 16),

ARC 104

(STRUCTURE 17),

ARC 123

(STRUCTURE 18),

ARC 124

(STRUCTURE 19),

AKC 125

(STRUCTURE 20),

AKC 105

(STRUCTURE 21),

AKC 126

(STRUCTURE 22),

AKC 102

(STRUCTURE 23),

AKC 103

(STRUCTURE 24), and

AKC 171

(STRUCTURE 25).

&null;0028&null; 3. A secondary arylamine of the following Formula III:

Ar&null;NH&null;CHR&null;CH2&null;CO&null;Y&null;&null;(Formula III)

&null;0029&null; wherein Ar is aryl or heteroaryl optionally substituted by one or more R3 groups;

&null;0030&null; R is aryl, heteroaryl, or heterocycloalkyl optionally substituted by R3;

&null;0031&null; Y is C1-6 alkyl, aryl, or heteroaryl optionally substituted by R3;

&null;0032&null; or R and Y together form a cycloalkyl or heterocycloalkyl ring; and

&null;0033&null; R3 is OH, halogen, CF3, OCF3, or a group selected from NH2, SO2 alkyl, C6-10 aryl, C1-6 alkoxy, and C1-6 alkyl, said group being optionally substituted by OH, C1-6 alkoxy, C1-6 alkyl, phenyl, halogen, or CF3.

&null;0034&null; Particularly preferred anthelmintic compounds according to Formula III are exemplified herein by compounds represented by structures 26-31 (depicted in FIGS. 26-31, respectively), which have been assigned the respective reference numbers:

3

AKC 128

(STRUCTURE 26),

AKC 129

(STRUCTURE 27),

AKC 130

(STRUCTURE 28),

AKC 131

(STRUCTURE 29),

AKC 132

(STRUCTURE 30), and

AKC 133

(STRUCTURE 31).

&null;0035&null; 4. A diaryl amine of the following Formula IV:

Ar&null;(Z)0-1&null;Ar&null;(CH2)0-1&null;NHR&null;&null;(Formula IV)

&null;0036&null; wherein Ar is aryl or heteroaryl optionally substituted by one or more R3 groups;

&null;0037&null; Z is NH, O, S, or Alk; and Alk is a linear or cyclic alkylene radical of up to 8 C atoms

&null;0038&null; wherein said radical optionally includes one or more heteroatoms;

&null;0039&null; R is H or R3,

&null;0040&null; R3 is OH, halogen, CF3, OCF3, or a group selected from NH2, SO2 alkyl, C6-10 aryl, C1-6 alkoxy, and C1-6 alkyl, said group being optionally substituted by OH, C1-6 alkoxy, C1-6 alkyl, phenyl, halogen, or CF3.

&null;0041&null; Particularly preferred anthelmintic compounds according to Formula IV are exemplified by compounds represented by structures 32-37 (depicted in FIGS. 32-37, respectively), which have been assigned the respective reference numbers:

4

AKC 109

(STRUCTURE 32),

AKC 134

(STRUCTURE 33),

AKC 135

(STRUCTURE 34),

AKC 136

(STRUCTURE 35),

AKC 137

(STRUCTURE 36), and

AKC 138

(STRUCTURE 37).

&null;0042&null; 5. A substituted heteropolycyclic compound of the following Formula V:

Het2&null;Q&null;&null;(Formula V)

&null;0043&null; wherein Het2 is two or three fused aromatic rings including one or more heteroatoms selected from N, O and S, and Q includes at least one substituent selected from OH, COOR3 and CONHR3, and optionally also another substituent selected from alkyl and alkenyl of up to 10 C atoms;

&null;0044&null; wherein R3 is OH, halogen, CF3, OCF3, or a group selected from NH2, SO2 alkyl, C6-10 aryl, C1-6 alkoxy, and C1-6 alkyl, said group being optionally substituted by OH, C1-6 alkoxy, C1-6 alkyl, phenyl, halogen, or CF3.

&null;0045&null; Particularly preferred anthelmintic compounds according to Formula V are exemplified by compounds represented by structures 38-43 (depicted in FIGS. 38-43, respectively), which have been assigned the respective reference numbers:

5

AKC 139

(STRUCTURE 38),

AKC 140

(STRUCTURE 39),

AKC 141

(STRUCTURE 40),

AKC 142

(STRUCTURE 41),

AKC 143

(STRUCTURE 42), and

AKC 144

(STRUCTURE 43).

&null;0046&null; For the foregoing Formulae I, II, III, IV, and V, as well as throughout this disclosure, the following definitions apply.

&null;0047&null; &null;Aryl&null; refers to an aromatic group, typically of 6-10 C atoms, such as phenyl or naphthyl.

&null;0048&null; &null;Alk&null; includes, for example, (CH2)n wherein n is an integer of up to 6, e.g. 1, 2, 3, or 4, or cyclohexylene.

&null;0049&null; &null;Heteroaryl&null; means an aromatic group including one or more heteroatoms selected from O, S and N. It will typically have 5 or 6 ring atoms. It may also be fused to one or more aryl groups. Examples are in the illustrated compounds.

&null;0050&null; &null;Heterocycloalkyl&null; means a cycloalkyl group in which one or more C atoms are replaced by one or more heteroatoms selected from O, S and N. It will typically have 5 or 6 ring atoms. Examples are in the illustrated compounds of structures 1-43.

&null;0051&null; Other preferred anthelmintic compounds useful according to the subject invention are represented by structures 44, 45, and 46 (depicted in FIGS. 44-46, respectively),and have been assigned the respective reference numbers:

6

AKC 145

(STRUCTURE 44),

AKC 146

(STRUCTURE 45), and

AKC 147

(STRUCTURE 46).

&null;0052&null; The invention process is particularly valuable to control nematodes which are pests to animals, as well as nematodes attacking the roots of desired crop plants, ornamental plants, and turf grasses. The desired crop plants can be, for example, cotton, soybean, tomatoes, potatoes, grapes, strawberries, bananas, or vegetables.

&null;0053&null; In one embodiment of the subject invention, the subject anthelmintic compounds are used in conjunction with one or more other nematicidal agents. The other nematicidal agent may be, for example, a biological agent, an avermectin, a milbemycin, or a fatty acid.

&null;0054&null; In another embodiment, the subject invention further provides methods for killing the eggs of nematodes. Thus, the subject invention further relates to the surprising discovery that certain compounds have ovicidal activity against nematode eggs. Compositions comprising the anthelmintic compounds of the subject invention are particularly useful for preplant applications in nematode-control schemes.

DESCRIPTION OF THE DRAWINGS

&null;0055&null; FIG. 1 depicts Structure 1 which represents anthelmintic compound AKC 111.

&null;0056&null; FIG. 2 depicts Structure 2 which represents anthelmintic compound AKC 112.

&null;0057&null; FIG. 3 depicts Structure 3 which represents anthelmintic compound AKC 112.

&null;0058&null; FIG. 4 depicts Structure 4 which represents anthelmintic compound AKC 107.

&null;0059&null; FIG. 5 depicts Structure 5 which represents anthelmintic compound AKC 114.

&null;0060&null; FIG. 6 depicts Structure 6 which represents anthelmintic compound AKC 108.

&null;0061&null; FIG. 7 depicts Structure 7 which represents anthelmintic compound AKC 115.

&null;0062&null; FIG. 8 depicts Structure 8 which represents anthelmintic compound AKC 116.

&null;0063&null; FIG. 9 depicts Structure 9 which represents anthelmintic compound AKC 117.

&null;0064&null; FIG. 10 depicts Structure 10 which represents anthelmintic compound AKC 118.

&null;0065&null; FIG. 11 depicts Structure 11 which represents anthelmintic compound AKC 119.

&null;0066&null; FIG. 12 depicts Structure 12 which represents anthelmintic compound AKC 110.

&null;0067&null; FIG. 13 depicts Structure 13 which represents anthelmintic compound AKC 120.

&null;0068&null; FIG. 14 depicts Structure 14 which represents anthelmintic compound AKC 121.

&null;0069&null; FIG. 15 depicts Structure 15 which represents anthelmintic compound AKC 2153.

&null;0070&null; FIG. 16 depicts Structure 16 which represents anthelmintic compound AKC 122.

&null;0071&null; FIG. 17 depicts Structure 17 which represents anthelmintic compound AKC 104.

&null;0072&null; FIG. 18 depicts Structure 18 which represents anthelmintic compound AKC 123.

&null;0073&null; FIG. 19 depicts Structure 19 which represents anthelmintic compound AKC 124.

&null;0074&null; FIG. 20 depicts Structure 20 which represents anthelmintic compound AKC 125.

&null;0075&null; FIG. 21 depicts Structure 21 which represents anthelmintic compound AKC 105.

&null;0076&null; FIG. 22 depicts Structure 22 which represents anthelmintic compound AKC 126.

&null;0077&null; FIG. 23 depicts Structure 23 which represents anthelmintic compound AKC 102.

&null;0078&null; FIG. 24 depicts Structure 24 which represents anthelmintic compound AKC 103.

&null;0079&null; FIG. 25 depicts Structure 25 which represents anthelmintic compound AKC 171.

&null;0080&null; FIG. 26 depicts Structure 26 which represents anthelmintic compound AKC 128.

&null;0081&null; FIG. 27 depicts Structure 27 which represents anthelmintic compound AKC 129.

&null;0082&null; FIG. 28 depicts Structure 28 which represents anthelmintic compound AKC 130.

&null;0083&null; FIG. 29 depicts Structure 29 which represents anthelmintic compound AKC 121.

&null;0084&null; FIG. 30 depicts Structure 30 which represents anthelmintic compound AKC 132.

&null;0085&null; FIG. 31 depicts Structure 31 which represents anthelmintic compound AKC 133.

&null;0086&null; FIG. 32 depicts Structure 32 which represents anthelmintic compound AKC 109.

&null;0087&null; FIG. 33 depicts Structure 33 which represents anthelmintic compound AKC 134.

&null;0088&null; FIG. 34 depicts Structure 34 which represents anthelmintic compound AKC 135.

&null;0089&null; FIG. 35 depicts Structure 35 which represents anthelmintic compound AKC 136.

&null;0090&null; FIG. 36 depicts Structure 36 which represents anthelmintic compound AKC 137.

&null;0091&null; FIG. 37 depicts Structure 37 which represents anthelmintic compound AKC 138.

&null;0092&null; FIG. 38 depicts Structure 38 which represents anthelmintic compound AKC 139.

&null;0093&null; FIG. 39 depicts Structure 39 which represents anthelmintic compound AKC 140.

&null;0094&null; FIG. 40 depicts Structure 40 which represents anthelmintic compound AKC 141.

&null;0095&null; FIG. 41 depicts Structure 41 which represents anthelmintic compound AKC 142.

&null;0096&null; FIG. 42 depicts Structure 42 which represents anthelmintic compound AKC 143.

&null;0097&null; FIG. 43 depicts Structure 43 which represents anthelmintic compound AKC 144.

&null;0098&null; FIG. 44 depicts Structure 44 which represents anthelmintic compound AKC 145.

&null;0099&null; FIG. 45 depicts Structure 45 which represents anthelmintic compound AKC 146.

&null;0100&null; FIG. 46 depicts Structure 46 which represents anthelmintic compound AKC 147.

&null;0101&null; FIG. 47 depicts a basic structure, Structure 47, of a preferred class of anthelmintic compound.

&null;0102&null; FIG. 48 depicts anthelmintic compound AKC 261 of the class represented in FIG. 47.

&null;0103&null; FIG. 49 depicts anthelmintic compound AKC 247 of the class represented in FIG. 47.

&null;0104&null; FIG. 50 depicts anthelmintic compound AKC 252 of the class represented in FIG. 47.

&null;0105&null; FIG. 51 depicts anthelmintic compound AKC 262 of the class represented in FIG. 47.

&null;0106&null; FIG. 52 depicts anthelmintic compound AKC 263 of the class represented in FIG. 47.

&null;0107&null; FIG. 53 depicts anthelmintic compound AKC 253 of the class represented in FIG. 47.

&null;0108&null; FIG. 54 depicts anthelmintic compound AKC 264 of the class represented in FIG. 47.

&null;0109&null; FIG. 55 depicts anthelmintic compound AKC 245 of the class represented in FIG. 47.

&null;0110&null; FIG. 56 depicts anthelmintic compound AKC 248 of the class represented in FIG. 47.

&null;0111&null; FIG. 57 depicts anthelmintic compound AKC 254 of the class represented in FIG. 47.

&null;0112&null; FIG. 58 depicts anthelmintic compound AKC 265 of the class represented in FIG. 47.

&null;0113&null; FIG. 59 depicts anthelmintic compound AKC 249 of the class represented in FIG. 47.

&null;0114&null; FIG. 60 depicts anthelmintic compound AKC 255 of the class represented in FIG. 47.

&null;0115&null; FIG. 61 depicts anthelmintic compound AKC 266 of the class represented in FIG. 47.

&null;0116&null; FIG. 62 depicts anthelmintic compound AKC 246 of the class represented in FIG. 47.

&null;0117&null; FIG. 63 depicts anthelmintic compound AKC 250 of the class represented in FIG. 47.

&null;0118&null; FIG. 64 depicts anthelmintic compound AKC 256 of the class represented in FIG. 47.

&null;0119&null; FIG. 65 depicts anthelmintic compound AKC 258 of the class represented in FIG. 47.

&null;0120&null; FIG. 66 depicts anthelmintic compound AKC 251 of the class represented in FIG. 47.

&null;0121&null; FIG. 67 depicts anthelmintic compound AKC 257 of the class represented in FIG. 47.

&null;0122&null; FIG. 68 depicts anthelmintic compound AKC 259 of the class represented in FIG. 47.

&null;0123&null; FIG. 69 depicts anthelmintic compound AKC 260 of the class represented in FIG. 47.

&null;0124&null; FIG. 70 depicts anthelmintic compound AKC 271 of the class represented in FIG. 47.

&null;0125&null; FIG. 71 depicts anthelmintic compound AKC 267 of the class represented in FIG. 47.

&null;0126&null; FIG. 72 depicts anthelmintic compound AKC 268 of the class represented in FIG. 47.

&null;0127&null; FIG. 73 depicts anthelmintic compound AKC 269 of the class represented in FIG. 47.

&null;0128&null; FIG. 74 depicts anthelmintic compound AKC 270 of the class represented in FIG. 47.

&null;0129&null; FIG. 75 depicts anthelmintic compound AKC 134 of the class represented in FIG. 47.

&null;0130&null; FIG. 76 depicts anthelmintic compound AKC 273 of the class represented in FIG. 47.

&null;0131&null; FIG. 77 depicts anthelmintic compound AKC 280 of the class represented in FIG. 47.

&null;0132&null; FIG. 78 depicts anthelmintic compound AKC 274 of the class represented in FIG. 47.

&null;0133&null; FIG. 79 depicts anthelmintic compound AKC 281 of the class represented in FIG. 47.

&null;0134&null; FIG. 80 depicts anthelmintic compound AKC 287 of the class represented in FIG. 47.

&null;0135&null; FIG. 81 depicts anthelmintic compound AKC 272 of the class represented in FIG. 47.

&null;0136&null; FIG. 82 depicts anthelmintic compound AKC 275 of the class represented in FIG. 47.

&null;0137&null; FIG. 83 depicts anthelmintic compound AKC 282 of the class represented in FIG. 47.

&null;0138&null; FIG. 84 depicts anthelmintic compound AKC 276 of the class represented in FIG. 47.

&null;0139&null; FIG. 85 depicts anthelmintic compound AKC 288 of the class represented in FIG. 47.

&null;0140&null; FIG. 86 depicts anthelmintic compound AKC 277 of the class represented in FIG. 47.

&null;0141&null; FIG. 87 depicts anthelmintic compound AKC 283 of the class represented in FIG. 47.

&null;0142&null; FIG. 88 depicts anthelmintic compound AKC 278 of the class represented in FIG. 47.

&null;0143&null; FIG. 89 depicts anthelmintic compound AKC 284 of the class represented in FIG. 47.

&null;0144&null; FIG. 90 depicts anthelmintic compound AKC 279 of the class represented in FIG. 47.

&null;0145&null; FIG. 91 depicts anthelmintic compound AKC 285 of the class represented in FIG. 47.

&null;0146&null; FIG. 92 depicts anthelmintic compound AKC 320 of the class represented in FIG. 47.

&null;0147&null; FIG. 93 depicts anthelmintic compound AKC 292 of the class represented in FIG. 47.

&null;0148&null; FIG. 94 depicts anthelmintic compound AKC 296 of the class represented in FIG. 47.

&null;0149&null; FIG. 95 depicts anthelmintic compound AKC 299 of the class represented in FIG. 47.

&null;0150&null; FIG. 96 depicts anthelmintic compound AKC 135 of the class represented in FIG. 47.

&null;0151&null; FIG. 97 depicts anthelmintic compound AKC 310 of the class represented in FIG. 47.

&null;0152&null; FIG. 98 depicts anthelmintic compound AKC 303 of the class represented in FIG. 47.

&null;0153&null; FIG. 99 depicts anthelmintic compound AKC 311 of the class represented in FIG. 47.

&null;0154&null; FIG. 100 depicts anthelmintic compound AKC 289 of the class represented in FIG. 47.

&null;0155&null; FIG. 101 depicts anthelmintic compound AKC 318 of the class represented in FIG. 47.

&null;0156&null; FIG. 102 depicts anthelmintic compound AKC 321 of the class represented in FIG. 47.

&null;0157&null; FIG. 103 depicts anthelmintic compound AKC 293 of the class represented in FIG. 47.

&null;0158&null; FIG. 104 depicts anthelmintic compound AKC 300 of the class represented in FIG. 47.

&null;0159&null; FIG. 105 depicts anthelmintic compound AKC 304 of the class represented in FIG. 47.

&null;0160&null; FIG. 106 depicts anthelmintic compound AKC 312 of the class represented in FIG. 47.

&null;0161&null; FIG. 107 depicts anthelmintic compound AKC 290 of the class represented in FIG. 47.

&null;0162&null; FIG. 108 depicts anthelmintic compound AKC 297 of the class represented in FIG. 47.

&null;0163&null; FIG. 109 depicts anthelmintic compound AKC 305 of the class represented in FIG. 47.

&null;0164&null; FIG. 110 depicts anthelmintic compound AKC 313 of the class represented in FIG. 47.

&null;0165&null; FIG. 111 depicts anthelmintic compound AKC 322 of the class represented in FIG. 47.

&null;0166&null; FIG. 112 depicts anthelmintic compound AKC 294 of the class represented in FIG. 47.

&null;0167&null; FIG. 113 depicts anthelmintic compound AKC 306 of the class represented in FIG. 47.

&null;0168&null; FIG. 114 depicts anthelmintic compound AKC 314 of the class represented in FIG. 47.

&null;0169&null; FIG. 115 depicts anthelmintic compound AKC 323 of the class represented in FIG. 47.

&null;0170&null; FIG. 116 depicts anthelmintic compound AKC 295 of the class represented in FIG. 47.

&null;0171&null; FIG. 117 depicts anthelmintic compound AKC 301 of the class represented in FIG. 47.

&null;0172&null; FIG. 118 depicts anthelmintic compound AKC 307 of the class represented in FIG. 47.

&null;0173&null; FIG. 119 depicts anthelmintic compound AKC 315 of the class represented in FIG. 47.

&null;0174&null; FIG. 120 depicts anthelmintic compound AKC 319 of the class represented in FIG. 47.

&null;0175&null; FIG. 121 depicts anthelmintic compound AKC 308 of the class represented in FIG. 47.

&null;0176&null; FIG. 122 depicts anthelmintic compound AKC 316 of the class represented in FIG. 47.

&null;0177&null; FIG. 123 depicts anthelmintic compound AKC 291 of the class represented in FIG. 47.

&null;0178&null; FIG. 124 depicts anthelmintic compound AKC 298 of the class represented in FIG. 47.

&null;0179&null; FIG. 125 depicts anthelmintic compound AKC 309 of the class represented in FIG. 47.

&null;0180&null; FIG. 126 depicts anthelmintic compound AKC 317 of the class represented in FIG. 47.

&null;0181&null; FIG. 127 depicts anthelmintic compound AKC 324 of the class represented in FIG. 47.

&null;0182&null; FIG. 128 depicts anthelmintic compound AKC 326 of the class represented in FIG. 47.

&null;0183&null; FIG. 129 depicts anthelmintic compound AKC 327 of the class represented in FIG. 47.

&null;0184&null; FIG. 130 depicts anthelmintic compound AKC 329 of the class represented in FIG. 47.

&null;0185&null; FIG. 131 depicts anthelmintic compound AKC 334 of the class represented in FIG. 47.

&null;0186&null; FIG. 132 depicts anthelmintic compound AKC 325 of the class represented in FIG. 47.

&null;0187&null; FIG. 133 depicts anthelmintic compound AKC 330 of the class represented in FIG. 47.

&null;0188&null; FIG. 134 depicts anthelmintic compound AKC 335 of the class represented in FIG. 47.

&null;0189&null; FIG. 135 depicts anthelmintic compound AKC 328 of the class represented in FIG. 47.

&null;0190&null; FIG. 136 depicts anthelmintic compound AKC 331 of the class represented in FIG. 47.

&null;0191&null; FIG. 137 depicts anthelmintic compound AKC 336 of the class represented in FIG. 47.

&null;0192&null; FIG. 138 depicts anthelmintic compound AKC 332 of the class represented in FIG. 47.

&null;0193&null; FIG. 139 depicts anthelmintic compound AKC 333 of the class represented in FIG. 47.

&null;0194&null; FIG. 140 depicts anthelmintic compound AKC 337 of the class represented in FIG. 47.

&null;0195&null; FIG. 141 depicts anthelmintic compound AKC 338 of the class represented in FIG. 47.

&null;0196&null; FIG. 142 depicts anthelmintic compound AKC 218 of the class represented in FIG. 47.

&null;0197&null; FIG. 143 depicts anthelmintic compound AKC 219 of the class represented in FIG. 47.

&null;0198&null; FIG. 144 depicts anthelmintic compound AKC 221 of the class represented in FIG. 47.

&null;0199&null; FIG. 145 depicts anthelmintic compound AKC 220 of the class represented in FIG. 47.

&null;0200&null; FIG. 146 depicts anthelmintic compound AKC 222 of the class represented in FIG. 47.

&null;0201&null; FIG. 147 depicts anthelmintic compound AKC 223 of the class represented in FIG. 47.

&null;0202&null; FIG. 148 depicts anthelmintic compound AKC 226 of the class represented in FIG. 47.

&null;0203&null; FIG. 149 depicts anthelmintic compound AKC 224 of the class represented in FIG. 47.

&null;0204&null; FIG. 150 depicts anthelmintic compound AKC 225 of the class represented in FIG. 47.

&null;0205&null; FIG. 151 depicts anthelmintic compound AKC 231 of the class represented in FIG. 47.

&null;0206&null; FIG. 152 depicts anthelmintic compound AKC 227 of the class represented in FIG. 47.

&null;0207&null; FIG. 153 depicts anthelmintic compound AKC 232 of the class represented in FIG. 47.

&null;0208&null; FIG. 154 depicts anthelmintic compound AKC 228 of the class represented in FIG. 47.

&null;0209&null; FIG. 155 depicts anthelmintic compound AKC 233 of the class represented in FIG. 47.

&null;0210&null; FIG. 156 depicts anthelmintic compound AKC 229 of the class represented in FIG. 47.

&null;0211&null; FIG. 157 depicts anthelmintic compound AKC 234 of the class represented in FIG. 47.

&null;0212&null; FIG. 158 depicts anthelmintic compound AKC 230 of the class represented in FIG. 47.

&null;0213&null; FIG. 159 depicts anthelmintic compound AKC 235 of the class represented in FIG. 47.

&null;0214&null; FIG. 160 depicts anthelmintic compound AKC 237 of the class represented in FIG. 47.

&null;0215&null; FIG. 161 depicts anthelmintic compound AKC 238 of the class represented in FIG. 47.

&null;0216&null; FIG. 162 depicts anthelmintic compound AKC 236 of the class represented in FIG. 47.

&null;0217&null; FIG. 163 depicts anthelmintic compound AKC 239 of the class represented in FIG. 47.

&null;0218&null; FIG. 164 depicts anthelmintic compound AKC 240 of the class represented in FIG. 47.

&null;0219&null; FIG. 165 depicts anthelmintic compound AKC 241 of the class represented in FIG. 47.

&null;0220&null; FIG. 166 depicts anthelmintic compound AKC 244 of the class represented in FIG. 47.

&null;0221&null; FIG. 167 depicts anthelmintic compound AKC 242 of the class represented in FIG. 47.

&null;0222&null; FIG. 168 depicts anthelmintic compound AKC 243 of the class represented in FIG. 47.

&null;0223&null; FIG. 169 depicts one library scheme by which the skilled artisan can create compounds represented by the structure depicted in FIG. 47.

DETAILED DISCLOSURE OF THE INVENTION

&null;0224&null; The process of the subject invention concerns the use of certain organic compounds to control the infestation of plants or animals by nematodes. These organic compounds comprise Formulae I, II, III, IV, and V, as well as Structures 44, 45, and 46. In a particularly preferred embodiment of the subject invention, the anthelmintic compound is selected from the group consisting of Compounds 1-46 represented by Structures 1-46. Particularly preferred is the compound represented by Structures 33 and 34, and compounds related thereto as represented by Structure 47 depicted in FIG. 47, and as further exemplified by Structures 48-168 depicted in FIGS. 48 through 168. Preferred anthelmintic compounds useful in accord with the subject invention are represented by Structure 47, wherein:

&null;0225&null; R1 is H; C2-5 alkenyl; C2-5 ether; C2-8 ester; or C1-20 straight or branched alkyl which is optionally substituted with OH, C3-12 cycloalkyl, or aryl (optionally substituted with halogenated alkyl, halogen, OC1-5 alkyl, OAr, or C1-5 alkyl);

&null;0226&null; R2 is H; C2-5 alkenyl; C2-5 ether; C2-8 ester; or C1-20 straight or branched alkyl which is optionally substituted with OH, C3-12 cycloalkyl, or aryl (optionally substituted with halogenated alkyl, halogen, OC1-5 alkyl, OAr, or C1-5 alkyl);

&null;0227&null; or R1 and R2 form a heterocycle which is optionally substituted with alkyl, halogenated alkyl, OH, C1-5 alcohol, amide, aryl (optionally substituted with halogenated alkyl, halogen, or alkyl), or heteroaryl;

&null;0228&null; R3 is H; C2-5 alkenyl; C2-5 ether; C2-8 ester; or C1-20 straight or branched alkyl which is optionally substituted with OH, C3-12 cycloalkyl, or aryl (optionally substituted with halogenated alkyl, halogen, OC1-5 alkyl, OAr, or C1-5 alkyl);

&null;0229&null; R4 is H; C2-5 alkenyl; C2-5 ether; C2-8 ester; or C1-20 straight or branched alkyl which is optionally substituted with OH, C3-12 cycloalkyl, or aryl (optionally substituted with halogenated alkyl, halogen, OC1-5 alkyl, OAr, or C1-5 alkyl);

&null;0230&null; or R3 and R4 form a heterocycle which is optionally substituted with alkyl, halogenated alkyl, OH, C1-5 alcohol, amide, aryl (optionally substituted with halogenated alkyl, halogen, or alkyl), or heteroaryl. Generally, the anthelmintic compounds of the subject invention can be unsubstituted or substituted, saturated or unsaturated. The anthelmintic component of an anthelmintic composition used according to the subject invention may be a single anthelmintic compound or a mixture of two or more anthelmintic compounds. The subject compounds may be used in conjunction with other anthelmintic compounds, including the free acids and salts of the anthelmintic compounds of the present invention. The salts may be, for example, sodium or potassium salts, or ammonium salts. As would be apparent to the ordinary skilled artisan, physiologically acceptable acids and salts of the subject anthelmintic compounds can readily be made and used in accord with the teachings herein, and are hereby expressly included by reference to each compound or group of compounds. For example, &null;AKC 261&null;, &null;Compound 48&null;, or &null;Structure 48&null; each refer to the same compounds and each is intended to include the physiologically acceptable acids and salts thereof.

&null;0231&null; Anthelmintic compounds specifically exemplified herein include Compounds 1-46 represented by Structures 1-46 above, and Compounds 48-168 represented by Structures 48-168 depicted in FIGS. 48-168.

&null;0232&null; The subject compounds used in the invention can be applied to animals, the living and feeding areas of animals, plants, or to the situs of plants needing nematode control. The anthelmintic compositions may be applied by, for example, drip and drench techniques. With the drip application, the subject compositions can be applied directly to the base of plants or to the soil root zone. The composition may be applied through already existing drip irrigation systems. This procedure is particularly applicable for ornamental plants, strawberries, tomatoes, potatoes, grapes, and vegetables. Alternatively, a drench application can be used. For treating plants, a sufficient quantity of the anthelmintic composition is applied such that the composition drains to the root area of the plants. An important aspect of the subject invention is the surprising discovery that certain compounds have excellent nematicidal activity at concentrations which are not phytotoxic.

&null;0233&null; The drench technique can be used for a variety of crops and for turf grasses. The drench technique can also be used for animals. Preferably, for administration to animals the anthelmintic composition would be administered orally to facilitate activity against internal nematode parasites. The compositions of the subject invention can readily be applied using the teachings provided herein.

&null;0234&null; In a preferred embodiment of the subject invention, an anthelmintic compound will be applied as an aqueous microemulsion. As described herein, the concentration of the active ingredient should be sufficient to control the nematode infestation without causing phytotoxicity to the desired plants. The concentration of anthelmintic compound may be, for example, from about 0.0001% to about 2%, preferably from about 0.025% to about 1%, and, most preferably, from about 0.05% to about 0.5%.

&null;0235&null; The anthelmintic composition used according to the subject invention can be applied in conjunction with one or more other nematicidal agents. The other nematicidal agent may, for example, be applied simultaneously or sequentially with the anthelmintic. Such other nematicidal agents include, for example, avermectins, the B.t.s, and fatty acids.

&null;0236&null; The avermectin compound used according to the subject invention may be any of the avermectins, milbemycins, or derivatives of either, having activity against nematodes. The avermectin's activity will be enhanced when combined with an anthelmintic compound as described herein. Thus, the specific combination of ingredients can be manipulated to provide the optimal composition for a particular application.

&null;0237&null; Standard concentrations of avermectins are well known to those skilled in the art. For example, the avermectin compounds can be employed in the combination of the subject invention at concentrations of from about 0.03 to about 110 parts per million (ppm). Preferably, from about 1 to about 5 ppm are employed.

&null;0238&null; As would be readily appreciated by a person skilled in the art, the delivery of the subject anthelmintic and/or avermectin compound can be calculated in terms of the active ingredient applied per unit area. For example, the subject anthelmintic may be applied at a rate of about 0.02 lb/acre to about 0.1 lb/acre and, preferably, from about 0.5 lb/acre to about 2 lbs/acre. Similarly, the avermectin product can be applied at a rate of up to about 16 oz. of formulated product (&null;AVID,&null; available from Merck) per acre. Preferably, about 4 oz. to about 8 oz. formulated &null;AVID&null; per acre would be used. Thus, the avermectin compound can be applied up to about 0.02 lb/acre. Preferably, the rate of avermectin is between about 0.005 lb/acre and 0.01 lb/acre. A person of ordinary skill in the art would readily appreciate that the desired application rate of the active ingredients could be achieved using a great variety of different concentrations of active ingredients while varying the application rate of the solution. Thus, a large quantity of dilute solution could be applied or a smaller quantity of a more concentrated solution.

&null;0239&null; A variety of different avermectins or related compounds can be used according to the subject invention. Ivermectin may also be used according to the subject invention, as may the milbemycins. For brevity, the term &null;avermectin&null; is used herein to refer to all the avermectins and their derivatives as well as related compounds such as the milbemycins and the ivermectins. &null;Derivatives&null; refer to chemical modifications of the avermectins or milbemycins which are well known and available to those skilled in this art. Such derivatives are described, for example, in U.S. Pat. No. 4,560,677. Avermectin is readily available under a variety of tradenames including &null;AVID,&null; &null;ZEPHYR,&null; &null;VERTIMEC,&null; and &null;AGRI-MEK.&null;

&null;0240&null; The anthelmintic compositions of the subject invention may also be used in conjunction with nematicidal agents other than the avermectins. For example, the anthelmintic compounds may be used with biological agents such as Bacillus thuringiensis or with nematicidal fungi. In this context, the anthelmintic composition could be applied at concentrations which would not antagonize the action of the biological agent. The biologically active agent may be in a live proliferative form or may be in a dead stabilized form as described, for example, in U.S. Pat. Nos. 4,695,462 and 4,695,455. Furthermore, the anthelmintic compositions of the subject invention may be used with plants which are specifically bred or engineered for nematode resistance. The plants may, for example, be transformed with B.t. genes which confer nematode resistance or may simply be hybrids or varieties selected for such resistance. The anthelmintic compositions of the subject invention are particularly effective against free-living ectoparasitic nematodes and, therefore, combined use with plants selected for endoparasitic nematode resistance is highly advantageous.

&null;0241&null; The subject invention further relates to the surprising discovery that the anthelmintics of the subject invention have ovicidal activity against nematode eggs. Thus, in another embodiment, provided are methods for killing the eggs of nematodes, including those within cysts or egg masses that are commonly formed by Heterodera, Globodera, and Meloidogyne (cyst and root-knot) species.

&null;0242&null; The ovicidal compositions according to the subject invention are particularly useful for preplant applications in nematode-control schemes. In addition, the ovicidal compositions of the subject invention can be advantageously used as postplant nematicides, especially because of their relatively low phytotoxicity. In the latter embodiments, ovicidal compositions of the subject invention can be delivered, after planting and at appropriate, essentially non-phytotoxic concentrations of anthelmintic compounds, along with irrigation water and/or plant nutrients to ensure a continuous zone of nematode protection to the enlarging plant root mass. Thus, when applied using these techniques, which include drench or drip systems as are known in the art, phytopathogenic nematodes in their vermiform (wormlike) and egg stages are controlled.

&null;0243&null; Anthelmintic compounds having Formulae I, II, III, IV, and V, Structure 47 and most preferably Structures 1-46, and particularly Structures 33 and 34, and Structures 48-168, are used in preferred embodiments for killing nematode eggs. In addition, microemulsions of the subject compounds are highly preferred for ovicidal applications. In preferred embodiments, the anthelmintic compound(s) will be present in a concentration of greater than about 150 ppm. More preferably, the concentration will be greater than about 200 ppm; most preferably it will be about 250 ppm or more. For certain conditions, the anthelmintic compounds should be applied at high concentrations of about 1,000 ppm to about 5,000 ppm or more.

&null;0244&null; In light of the subject disclosure, one skilled in the art could readily use a variety of application techniques and formulations to prevent the hatching of nematode eggs in a variety of agricultural, farm-related, and garden-related settings.

&null;0245&null; Examples of animal parasitic nematodes against which the subject compounds can be used include the following:

&null;0246&null; Amblyomma spp.

&null;0247&null; Babesia spp. (RBC)

&null;0248&null; Bunostomum spp.

&null;0249&null; Calliphorid larvae

&null;0250&null; Capillaria spp.

&null;0251&null; Chabertia ovina

&null;0252&null; Chorioptes

&null;0253&null; Cooperia spp.

&null;0254&null; Cryptosporidium sp.

&null;0255&null; Damalinia ovis

&null;0256&null; Damalinia caprae

&null;0257&null; Demodex

&null;0258&null; Dermacentor spp.

&null;0259&null; Dicrocoelium dentriticum

&null;0260&null; Dictyocaulus filaria

&null;0261&null; Echinococcus hydatid cyst

&null;0262&null; Eimeria spp.

&null;0263&null; Elaeophora schneideri

&null;0264&null; Fasciola hepatica

&null;0265&null; Fasciola gigantica

&null;0266&null; Fascioloides magna

&null;0267&null; Giardia sp.

&null;0268&null; Gongylonema spp.

&null;0269&null; Haematobia irritans

&null;0270&null; Haemonchus contortus contortus

&null;0271&null; Ixodes

&null;0272&null; Linguatula serrata larvae

&null;0273&null; Linguatula serrata nymphs

&null;0274&null; Linognathus spp.

&null;0275&null; M. domestica

&null;0276&null; Marshallagia marshalli

&null;0277&null; Melophagus ovinus

&null;0278&null; Moniezia benedeni

&null;0279&null; Moniezia expansa

&null;0280&null; Muellerius capillaris

&null;0281&null; Musca autumnalis

&null;0282&null; Nematodirus spp.

&null;0283&null; Oesophagostomum spp.

&null;0284&null; Oestrus ovis

&null;0285&null; Ornithodoros

&null;0286&null; Ostertagia circumcincta

&null;0287&null; Ostertagia trifurcata

&null;0288&null; Otobius

&null;0289&null; Paramphistomum sp.

&null;0290&null; Parelaphostrongylus tenuis

&null;0291&null; Protostrongylus sp.

&null;0292&null; Psoroptes

&null;0293&null; Rhipicephalus spp.

&null;0294&null; Sarcoptes scabiei

&null;0295&null; Sarcocystis spp.

&null;0296&null; Sarcocystis spp. cysts

&null;0297&null; Schistosoma spp.

&null;0298&null; Stomoxys calcitrans

&null;0299&null; Strongyloides papillosus

&null;0300&null; Taenia hydatigena cysticerci

&null;0301&null; Taenia multiceps coenurus

&null;0302&null; Taenia ovis cysticerci

&null;0303&null; Thelazia

&null;0304&null; Thysanosoma actinoides

&null;0305&null; Theileria spp.C)

&null;0306&null; Toxocara vitulorum

&null;0307&null; Toxoplasma gondii

&null;0308&null; Toxoplasma gondii cysts

&null;0309&null; Trichostrongylus axei

&null;0310&null; Trichostrongylus spp.

&null;0311&null; Trichunis ovis

&null;0312&null; Trypanosoma spp. (plasma)

&null;0313&null; It has been found that helminth, acarid and arthropod endo- and ectoparasitic infestations may be controlled, prevented or eliminated, by applying to, injecting or orally dosing said animals with an endo- or ectoparasiticidally effective amount of the subject anthelmintic compounds, preferably the above-described structure 1-46 compounds. This may be achieved by applying the compound to the skin, hide and/or hair of the animals, or injecting or orally dosing said animals with a solid or liquid formulated composition.

&null;0314&null; For control of flea infestations, treatment of the infested animal to control adults in conjunction with treatment of the area occupied by the infested animal to control flea larvae is recommended. The compositions of the present invention may be admixed with suitable carriers for application to interior and/or exterior areas for control of flea larvae.

&null;0315&null; The compositions of the present invention may be employed as animal feeds, animal feed premixes or feed concentrates. Feed concentrates and feed premixes, useful in the practice of the invention, may be prepared by admixing about 0.25% to 35% by weight of a subject anthelmintic compound, preferably a structure 1-46 compound, with about 99.75% to 65% by weight of a suitable agronomic carrier or diluent. Carriers suitable for use include 0.75% to 35% by weight of a physiologically acceptable alcohol such as benzyl alcohol, phenethyl alcohol or propylene glycol, 0 to about 10% by weight of a vegetable oil such as corn oil or soybean oil, or propylene glycol and about 30% to 95% by weight of a sorptive, edible organic carrier such as corn grits, wheat middlings, soybean meal, expanded corn grits, extracted corn meal or the like or a sorptive silica or a silicate. These feed premixes or concentrates may be admixed with the appropriate amount of animal feed to provide the animals with about 0.5 ppm to 1,000 ppm and preferably about 1 ppm to 500 ppm ofthe compound in the animal's diet. These premixes or concentrates may also be used as top dressings for the animal's daily ration and applied across the top of the daily ration in sufficient amount to provide the animal with about 0.5 ppm to 1,000 ppm and preferably about 1 ppm to 500 ppm of the active ingredient, based on the animal's total feed.

&null;0316&null; The subject anthelmintic compounds, and particularly the Structure 1-46 compounds, most particularly Structures 33 and 34 and Structure 48-168 compounds, may be administered to the animals in or with their drinking water.

&null;0317&null; The compound may also be administered in the form of a pill, tablet, bolus, implant, capsule, or drench, containing sufficient anthelmintic compound to provide the treated animal with about 0.01 mg/kg to 100 mg/kg of animal body weight per day of the compound. These dosage forms are prepared by intimately and uniformly mixing the active ingredient with suitable finely divided diluents, fillers, disintegrating agents and/or builders such as starch, lactose, talc, magnesium stearate, vegetable gums, or the like. These unit dosage formulations may be varied with respect to the total weight and content of anthelmintic compound depending upon the kind and size of the animal to be treated, the severity or type of infection encountered and the weight of the host.

&null;0318&null; Alternatively, the anthelmintic compound may be administered to animals parenterally, for example, by intraruminal, intramuscular, or subcutaneous injection in which the active ingredient is dissolved or dispersed in a liquid carrier. For this type administration the compound may be dispersed in a physiologically acceptable solvent for subcutaneous injection, or it may be dispersed in a fat or wax or mixture thereof containing an oil, buffer, surfactant, stabilizer, preservative and salt. Components useful in these preparations include carbowax, aluminum monostearate gel, diethyl succinate, soya oil, glyceral dioleate, saline, and capric/caprylic triglycerides.

&null;0319&null; The subject anthelmintic compounds may also be applied topically to the larger animals such as swine, sheep, cattle, and horses and companion animals such as dogs and cats in the form of aqueous dips or sprays. For this type administration, the active compound is generally prepared as a wettable powder, emulsifiable concentrate, aqueous flowable, or the like, which is mixed with water at the site of treatment and applied topically to the hide, skin, or hair of the animal. Such sprays or dips usually contain about 0.5 ppm to 5,000 ppm and preferably about 1 ppm to 3,000 ppm of the compound.

&null;0320&null; Advantageously, the subject anthelmintic compounds may also be prepared as pour-on formulations and poured on the backs of the animals such as swine, cattle, sheep, horses, poultry, and companion animals to protect them against infe station by nematodes, acarids, and arthropod endo- and ectoparasites. Such pour-on compositions are generally prepared by dissolving, dispersing, or emulsifying the anthelmintic compound in a suitable nontoxic pharmacologically acceptable diluent for pour-on and administration. The diluent must be compatible with the compound and should not be a source of irritation or damage to the animals hide, skin, or hair. Such diluents include vegetable oils, spreading oils, polyhydric alcohols, aliphatic or aromatic hydrocarbons, esters of fatty acids, and lower alkyl ketones.

&null;0321&null; A typical pour-on formulation includes about 0.5% to 30% by weight of the anthelmintic compound, about 30% to 60% by weight of an aliphatic or aromatic hydrocarbon, mono or polyhydric alcohol, lower alkyl ketone or mixtures thereof, 0 to about 20% by weight of a vegetable or mineral oil and about 0.5% to 30% by weight of a spreading oil. Another typical pour-on contains about 45% by weight of xylene, about 15% by weight of the anthelmintic compound, about 10% by weight of corn oil or mineral oil, about 25% by weight of cyclohexanone and about 5% by weight of other pharmacologically acceptable spreading agents, antifoam agents, surfactants, or the like.

&null;0322&null; The subject anthelmintic compounds may also be prepared as ear tags for animals, particularly quadrupeds such as cattle and sheep. The tags may be prepared by stirring together about 55% to 60% by weight of a vinyl dispersion resin, having an inherent viscosity of about 1.20 and an average particle size of about 0.75 microns, a curing temperature range of about 120&null; C. to 180&null; C., with about 28% by weight of butylbenzylphthalate. Stirring is continued, and about 1.5% by weight of ca/Zn stearate stabilizer is added along with about 7.0% of the compound and 2.8% of epoxidized soybean oil. The resulting mixture is deaerated for 15 to 20 minutes at 125 mm/Hg. This mixture can be coated on an ear tag blank by dipping and the resulting tag cured at about 145&null; C. to 150&null; C. for about five minutes.

&null;0323&null; The compounds of Formulae I-V, Structure 47, particularly Structures 1-46, and particularly Structures 33, 34, and 48-168 are nematicidal and can be used to control nematodes in crop plants. Therefore, in a further preferred aspect of the invention, there is provided a method for killing or controlling nematodes which comprises applying to the locus of the pests or to a plant susceptible to attack by the pest an effective amount of a compound having any of Structures 1-46, preferably Structure 47, and particularly Structures 33, 34, and 48-168, as defined herein.

&null;0324&null; The term &null;controlling&null; extends to non-lethal effects which result in the reduction or prevention of damage to the host plant or animal and the limitation of nematode population increase. These effects may be the result of chemical induced disorientation, immobilisation, or hatch prevention or induction. The chemical treatment may also have deleterious effects on nematode development, reproduction, or viability.

&null;0325&null; The compounds of the invention can be used against both plant-parasitic nematodes and nematodes living freely in the soil. Examples of plant-parasiticnematodes are: ectoparasites, for example Xiphinema spp., Longidorus spp., and Trichodorous spp.; semi-endoparasites, for example, Tylenchulus spp.; migratory endoparasites, for example, Pratylenchus spp., Radopholus spp., and Scutellonema spp.; sedentary endoparasites, for example, Heterodera spp., Globodera spp., and Meloidogyne spp.; and stem and leaf endoparasites, for example, Ditylenchus spp., Aphelenchoides spp., and Hirshmaniella spp.

&null;0326&null; The Formulae I-V compounds, Structure 47 compounds, and preferably the compounds of Structures 1-46, more preferably the compounds of Structures 33, 34, and 48-168, display nematicidal activity against different types of nematodes including the cyst nematode. The subject compounds may also be used to combat and control infestations of insect pests such as Lepidoptera, Diptera, Homoptera, and Coleoptera (including Diabrotica i.e. corn rootworms) and also other invertebratepests, for example, acarine pests. The insect and acarine pests which may be combated and controlled by the use of the invention compounds include those pests associated with agriculture (which term includes the growing of crops for food and fiber products), horticulture and animal husbandry, forestry, the storage of products of vegetable origin, such as fruit, grain and timber, and also those pests associated with the transmission of diseases of man and animals. Examples of insect and acarine pest species which may be controlled by the subject compounds include:

&null;0327&null; Myzus persicae (aphid)

&null;0328&null; Aphis gossypii (aphid)

&null;0329&null; Aphis fabae (aphid)

&null;0330&null; Megoura viceae (aphid)

&null;0331&null; Aedes aegypti (mosquito)

&null;0332&null; Anopheles spp. (mosquitos)

&null;0333&null; Culex spp. (mosquitos)

&null;0334&null; Dysdercus fasciatus (capsid)

&null;0335&null; Musca domestica (housefly)

&null;0336&null; Pieris brassicae (white butterfly)

&null;0337&null; Plutella maculipennis (diamond back moth)

&null;0338&null; Phaedon cochleariae (mustard beetle)

&null;0339&null; Aonidiella spp. (scale insects)

&null;0340&null; Trialeuroides spp. (white flies)

&null;0341&null; Bemisia tabaci (white fly)

&null;0342&null; Blattella germanica (cockroach)

&null;0343&null; Periplaneta americana (cockroach)

&null;0344&null; Blatta orientalis (cockroach)

&null;0345&null; Spodoptera littoralis (cotton leafworm)

&null;0346&null; Hellothis virescens (tobacco budworm)

&null;0347&null; Chortiocetes terminifera (locust)

&null;0348&null; Diabrotica spp. (rootworms)

&null;0349&null; Agrotis spp. (cutworms)

&null;0350&null; Chilo partellus (maize stem borer)

&null;0351&null; Nilaparvata lugens (planthopper)

&null;0352&null; Nephotettix cincticeps (leafhopper)

&null;0353&null; Panonychus ulmi (European red mite)

&null;0354&null; Panonychus citri (citrus red mite)

&null;0355&null; Tetranychus urticae (two-spotted spider mite)

&null;0356&null; Tetranychus cinnabarinus (carmine spider mite)

&null;0357&null; Phyllcoptruta oleivora (citrus rust mite)

&null;0358&null; Polyphagotarsonemus latus (broad mite)

&null;0359&null; Brevipalpus spp. (mites)

&null;0360&null; In order to apply the compound to the locus of the nematode, insect, or acarid pest, or to a plant susceptible to attack by the nematode, insect, or acarid pest, the compound is usually formulated into a composition which includes in addition to at least one of the subject anthelmintic compounds suitable inert diluent or carrier materials, and/or surface active agents. Thus, in two further aspects of the invention there is provided a nematicidal, insecticidal, or acaricidal composition comprising an effective amount of a subject anthelmintic compound and preferably of any of Structures 1-46, preferably compounds of Structure 47, more preferably as exemplified by Structures 33, 34, and 48-168, as defined herein and an inert diluent or carrier material and optionally a surface active agent.

&null;0361&null; The amount of active ingredients generally applied for the control of nematode pests is from 0.01 to 10 kg per hectare, and preferably from 0.1 to 6 kg per hectare.

&null;0362&null; The compositions can be applied to the soil, plant or seed, to the locus of the pests, or to the habitat of the pests, in the form of dusting powders, wettable powders, granules (slow or fast release), emulsion or suspension concentrates, liquid solutions, emulsions, seed dressings, fogging/smoke formulations or controlled release compositions, such as microencapsulated granules or suspensions.

&null;0363&null; Dusting powders are formulated by mixing the active ingredient with one or more finely divided solid carriers and/or diluents, for example natural clays, kaolin, pyrophyllite, bentonire, alumina, montmorillonite, kieselguhr, chalk, diatomaceous earths, calcium phosphates, calcium and magnesium carbonates, sulphur, lime, flours, talc, and other organic and inorganic solid carriers.

&null;0364&null; Granules are formed either by absorbing the active ingredient in a porous granular material for example pumice, attapulgite clays, fullers earth, kieselguhr, diatomaceous earths, ground corn cobs, and the like, or on to hard core materials such as sands, silicates, mineral carbonates, sulphates, phosphates, or the like. Agents which are commonly used to aid in impregnation, binding or coating the solid carriers include aliphatic and aromatic petroleum solvents, alcohols, polyvinyl acetates, polyvinyl alcohols, ethers, ketones, esters, dextrins, sugars, and vegetable oils with the active ingredient. Other additives may also be included, such as emulsifying agents, wetting agents, or dispersing agents.

&null;0365&null; Microencapsulated formulations (microcapsule suspensions CS) or other controlled release formulations may also be used, particularly for slow release over a period of time, and for seed treatment.

&null;0366&null; Alternatively the compositions may be in the form of liquid preparations to be used as dips, irrigation additives or sprays, which are generally aqueous dispersions or emulsions of the active ingredient in the presence of one or more known wetting agents, dispersing agents or emulsifying agents (surface active agents). The compositions which are to be used in the form of aqueous dispersions or emulsions are generally supplied in the form of an emulsifiable concentrate (EC) or a suspension concentrate (SC) containing a high proportion of the active ingredient or ingredients. An EC is a homogeneous liquid composition, usually containing the active ingredient dissolved in a substantially non-volatile organic solvent. An SC is a fine particle size dispersion of solid active ingredient in water. To apply the concentrates they are diluted in water and are usually applied by means of a spray to the area to be treated. For agricultural or horticultural purposes, an aqueous preparation containing between 0.0001% and 0.1% by weight of the active ingredient (approximately equivalent to from 5-2000 g/ha) is particularly useful.

&null;0367&null; Suitable liquid solvents for ECs include methyl ketone, methyl isobutyl ketone, cyclohexanone, xylenes, toluene, chlorobenzene, paraffins, kerosene, white oil, alcohols, (for example, butanol), methylnaphthalene, trimethylbenzene, trichloroethylene, N-methyl-2-pyrrolidone, and tetrahydrofurfuryl alcohol (THFA).

&null;0368&null; Wetting agents, dispersing agents, and emulsifying agents may be of the cationic, anionic, or non-ionic type. Suitable agents of the cationic type include, for example, quaternary ammonium compounds, for example cetyltrimethyl ammonium bromide. Suitable agents of the anionic type include, for example, soaps; salts of aliphatic monoesters of sulphuric acid, for example sodium lauryl sulphate; salts of sulphonated aromatic compounds, for example sodium dodecylbenzenesulphonate; sodium, calcium or ammonium lignosulphonate; or butylnaphthalene sulphonate; and a mixture of the sodium salts of diisopropyl- and triisopropylnaphthalenesulphonates. Suitable agents of the non-ionic type include, for example, the condensation products of ethylene oxide with fatty alcohols such as oleyl alcohol or cetyl alcohol; or with alkyl phenols such as octyl phenol, nonyl phenol, and octyl cresol. Other non-ionic agents are the partial esters derived from long chain fatty acids and hexitol anhydrides, the condensation products of the said partial esters with ethylene oxide, and the lecithins.

&null;0369&null; These concentrates are often required to withstand storage for prolonged periods and after such storage, to be capable of dilution with water to form aqueous preparations which remain homogeneous for a sufficient time to enable them to be applied by conventional spray equipment. The concentrates may preferably contain 1-85% by weight of the active ingredient or ingredients. When diluted to form aqueous preparations such preparations may contain varying amounts of the active ingredient depending upon the purpose for which they are to be used.

&null;0370&null; The subject anthelmintic compounds may also be formulated as powders (dry seed treatment DS or water disperible powder WS) or liquids (flowable concentrate FS, liquid seed treatment LS), or microcapsule suspensions CS for use in seed treatments. The formulations can be applied to the seed by standard techniques and through conventional seed treaters. In use the compositions are applied to the nematodes, to the locus of the nematodes, to the habitat of the nematodes, or to growing plants liable to infestation by the nematodes, by any of the known means of applying pesticidal compositions, for example, by dusting, spraying, or incorporation of granules.

&null;0371&null; The compounds of the invention may be the sole active ingredient of the composition or they may be admixed with one or more additional active ingredients such as nematicides, agents which modify the behavior of nematodes (such as hatching factors), insecticides, synergists, herbicides, fungicides or plant growth regulators where appropriate.

&null;0372&null; Suitable additional active ingredients for inclusion in admixture with the compounds of the invention may be compounds which will broaden the spectrum of activity of the compounds of the invention or increase their persistence in the location of the pest. They may synergise the activity of the compound of the invention or complement the activity for example by increasing the speed of effect or overcoming repellency. Additionally multi-component mixtures of this type may help to overcome or prevent the development of resistance to individual components.

&null;0373&null; The particular additional active ingredient included will depend upon the intended utility of the mixture and the type of complementary action required. Examples of suitable insecticides include the following:

&null;0374&null; a) Pyrethroids such as permethrin, esfenvalerate, deltamethrin, cyhalothrin in particular lambda-cyhalothrin, biphenthrin, fenpropathrin, cyfluthrin, tefluthrin, fish safe pyrethroids for example ethofenprox, natural pyrethrin, tetramethrin, s-bioallethrin, fenfluthrin, prallethrin, and 5-benzyl-3-furylmethyl-(E)-(1R,3S)-2,2-dimethyl-3-(2-oxothiolan-3-ylidenem ethyl) cyclopropane carboxylate;

&null;0375&null; b) Organophosphates such as profenofos, sulprofos, methyl parathion, azinphos-methyl, demeton-s-methyl, heptenophos, thiometon, fenamiphos, monocrotophos, profenophos, triazophos, methamidophos, dimethoate, phosphamidon, malathion, chloropyrifos,phosalone, terbufos, fensulphothion, fonofos, phorate, phoxim, pyrimiphos-methyl, pyrimiphos-ethyl, fenitrothion, or diazinon;

&null;0376&null; c) Carbamates (including aryl carbamates) such as pirimicarb, cloethocarb, carbofuran, furathiocarb, ethiofencarb, aldicarb, thiofurox, carbosulphan, bendiocarb, fenobucarb, propoxur, or oxamyl;

&null;0377&null; d) Benzoyl ureas such as triflumuron or chlorofluazuron;

&null;0378&null; e) Organic tin compounds such as cyhexatin, fenbutatin oxide, or azocyclotin;

&null;0379&null; f) Macrolides such as avermectins or milbemycins, for example such as abamectin, avermectin, and milbemycin;

&null;0380&null; g) Hormones and pheromones;

&null;0381&null; h) Organochlorine compounds such as benzene hexachloride, DDT, endosulphan, chlordane, or dieldrin;

&null;0382&null; i) Amidines, such as chlordimeform or amitraz;

&null;0383&null; j) Fumigant agents;

&null;0384&null; k) nitromethylenes such as imidacloprid.

&null;0385&null; In addition to the major chemical classes of insecticide listed above, other insecticideshaving particular targets may be employed in the mixture if appropriate for the intended utility of the mixture. For instance, selective insecticides for particular crops, for example stemborer specific insecticides for use in rice such as cartap or buprofezin, can be employed. Alternatively, insecticides specific for particular insect species/stages, for example, ovo-larvicides such as chlofentezine, flubenzimine, hexythiazox, and tetradifon; motilicides such as dicofol or propargite; acaricides such as bromopropylate or chlorobenzilate;or growth regulators such as hydramethylon,cyromazin, methoprene, chlorfluazuron, and diflubenzuron may also be included in the compositions.

&null;0386&null; Examples of suitable synergists for use in the compositions include piperonyl butoxide, sesamax, safroxan, and dodecyl imidazole.

&null;0387&null; Suitable herbicides, fungicides, and plant-growth regulators for inclusion in the compositions will depend upon the intended target and the effect required.

&null;0388&null; An example of a rice selective herbicides which can be included is propanil, an example of a plant growth regulator for use in cotton is &null;Pix&null;, and examples of fungicides for use in rice include blasticides such as blasticidin-S. The ratio of the compound of the invention to the other active ingredient in the composition will depend upon a number of factors including type of target, effect required from the mixture, etc. However in general, the additional active ingredient of the composition will be applied at about the rate as it is usually employed, or at a slightly lower rate if synergism occurs.

&null;0389&null; The anthelmintic compounds according to the invention also show fungicidal activity and may be used to control one or more of a variety of plant pathogens. In a further aspect the invention therefore includes a method of combating fungi which comprises applying to a plant, to a seed of a plant, or to the locus of the plant or seed a fungicidally effective amount of a compound as herein defined or a composition containing the same. The invention further includes a fungicidal composition comprising a fungicidally effective amount of a compound as herein defined and a fungicidally acceptable carrier or diluent therefor.

&null;0390&null; Examples of plant pathogens which the compounds or fungicidal compositions of the invention may control, methods by which fungi may be combatted and the form of suitable compositions, including acceptable carriers and diluents; adjuvants such as wetting, dispersing, emulsifying, and suspending agents; and other ingredients, such as fertilisers and other biologically active materials, are described, for instance, in International application No. WO 93/08180, the content of which is incorporated herein by reference.

&null;0391&null; All of the U.S. patents cited herein are hereby incorporated by reference.

&null;0392&null; Following are examples which illustrate procedures for practicing the invention. These examples should not be construed as limiting. All percentages are by weight and all solvent mixture proportions are by volume unless otherwise noted. For clarity the following abbreviations shall be used throughout the examples:

7

ACD

Available Chemicals Directory

DMSO

Dimethylsulfoxide

dH2O

Distilled Water

EXAMPLE 1

Preparation of Anthelmintic Compounds 1-46

&null;0393&null; The anthelmintic compounds of the subject invention can readily be produced using procedures well known to those skilled in the art.

&null;0394&null; A variety of anthelmintic compounds useful according to the subject invention can be readily prepared by a person skilled in this art having the benefit of the subject disclosure.

EXAMPLE 2

Nematicidal Activity of Anthelmintic Compositions 1-31

&null;0395&null; Caenorhabditis elegans adults were grown on Nematode Growth Medium (NGM) until they produced eggs, then the adults were removed.

&null;0396&null; The eggs were allowed to hatch, and the L1 larvae collected. See The Nematode Caenorhabditis elegans (1988) Cold Spring Harbor Laboratory Press. Using a Matrix Programmable Pipette, the L1s were distributed into 96-well tissue culture plates, 20 L1 in 50 &null;l NGM per well. Antibiotic/Antimyoticwas added to each well, and 1% by weight E. coli strain HB101. The subject anthelmintic compounds were stored at 5 mM in 100% DMSO. 0.7 &null;l of compounds 1-31 were added to the left-most column of wells to yield a final concentration of 70 &null;M in 1.4% DMSO, with 1.4% DMSO only as the control. The compounds were then subjected to 5 more 3-fold dilutions from left to right to yield 6 column concentrations of 70 &null;M, 23.3 &null;M, 7.8 &null;M, 2.6 &null;M, 0.9 &null;M, and 0.3 &null;M. Plates were stored in air-tight Rubbermaid plastic boxes at 20&null; C. The nematodes had cleared all control wells by day 4, and nematode viability was scored by visual examination under a 100&null; dissecting microsope on day 5. A visual viability scoring system was used as follows:

WORM VISUAL SCORING GUIDE

&null;0397&null;

8

WORM VISUAL SCORING GUIDE

Lethality:

Dead

only stiff L1s (no movement)

Dead (L4)

worms are dead, but at a later larval stage

L1

majority of worms are L1 (based on size)

worms move when plate is tapped

L2

majority of worms are L2 (based on size)

L3

majority of worms are L3 (based on size)

L4

majority of worms are L4 (based on size)

Partial Penetrance:

AD

majority of worms are adult

&null;AD

5 adult worms or less

Broodsize Reductions:

B&null;

sterile

(0-25 progeny)

B

low broodsize

(25-100 progeny)

&null;B

moderate broodsize

(100-250 progeny)

<

reduced broodsize

(250-500 progeny)

OK

no effect

(&null;1000&null; progeny)

If several classes of worms exist in a well, then all classes are scored. If adults are present, then the brood score is also recorded. Thus, &null;L1/L2&null; would mean a mixture of L1&null;s and L2's are present in the well. &null;L4/&null;AD/B&null; would mean that a mixture of L4's and adults are resent in the well. The &null;&null;AD&null; would mean that there are 6 or less adults, and the &null;B&null; would mean that there were 100 progeny or less.

&null;0398&null; The results are reported in Table 1. Column V1 has a compound concentration of 70 &null;M with sequential 3-fold dilutions reported in columns V2, V3, V4, V5, and V6, respectively, such that the V6 concentration was 0.3 &null;M.

9

TABLE I

Dose Response Tracking

5 Day Visual Score

DR&null;

HTS Tracking Library &null;

Structure &null;

Source P

Well Address

V1

V2

V3

V4

V5

V6

1575

AKC 111

1

N2 &null;93

5081:D10

Dead

Dead

L2

Dead(L3/L

&null;AD/B

OK

1647

AKC 112

2

N2 &null;98

5090:A10

L2/L3

L2/L3

L2/L3

L3/L4

&null;AD/&null;B

OK

1466

AKC 113

3

N2 &null;85

5061:A10

Dead

Dead

Dead

Dead(L2/L

L2/Dead(A

L4/&null;AD/B

1469

AKC 107

4

N2 &null;86

5061:D10

Dead

Dead(L2/L3)

L2/L3

Dead(L4)

L4/Dead(A

L2/Dead(L

1477

AKC 114

5

N2 &null;86

5061:D11

Dead

L3/Dead(L4)

L3

L3/Dead(L

L2/L3

&null;B

1476

AKC 108

6

N2 &null;86

5061:C1

Dead

Dead

L1

L1

L1/L2

L2/L3

1473

AKC 115

7

N2 &null;86

5061:H10

Dead

Dead(L2/L3)

L2/Dead(L2)

Dead(L2)

Dead(L2/L

L2/L3

&null;035

AKC 119

11

N2 &null;126

5393.B4

&null;AD/B

&null;AD/B

&null;AD/B

&null;AD/B

&null;AD/B

<

2059

AKC 110

12

N2 &null;128

5399.C4

L1

L1

L1

L1

L1

L1

2083

AKC 120

13

N2 &null;130

5419:C4

L1

L2/L3

L1

L1/L2

&null;AD/&null;B

OK

2032

AKC 121

14

N2 &null;126

5389:C4

L1

L1

L1/L2

&null;AD/&null;B

&null;AD/B

<

2029

AKC 2153

15

N2 &null;126

5379:C4

L1

L1

L1/L2

&null;AD/B

&null;AD/B

&null;AD/B

1962

AKC 122

16

N2 &null;121

5373:B8

Dead

L1

&null;AD/B

&null;AD/B

&null;AD/B

OK

1388

AKC 104

17

N2 &null;80

5022:C4

L1/L2

L1/L2

L1/L2

&null;AD/B

L1/L2

L1/L2

1372

AKC 123

18

N2 &null;79

5016.B8

L1

L1/L2

&null;AD/B

<

&null;AD/B

&null;AD/&null;B

1402

AKC 124

19

N2 &null;81

5033:D8

&null;AD/B&null;

&null;AD/B&null;

L2/L3

L4/&null;AD/B

L4/&null;AD/B

L4/&null;AD/B

1396

AKC 125

20

N2 &null;80

5031:G8

L2/Dead(L3)

L2/Dead(L4)

L2

L2

&null;AD/B

<

1393

AKC 105

21

N2 &null;80

5031.G2

L2/Dead(L3)

L2/Dead(L3)

L2/Dead(L3)

L2/Dead(L

L2/Dead(A

1164

AKC 126

22

N2 &null;64

4724:E10

L1/L2

L1/L2

L1/L2

&null;AD/&null;B

&null;B

&null;AD/&null;B

1174

AKC 102

23

N2 &null;65

4727:E8

L1/L2

L1/L2

L2/L3

L3

&null;AD/B

B

&null;806

AKC 103

24

N2 &null;149

4470:D10

L1/L2

Dead(L3/L4)

Dead(L4)

B

L4/&null;AD/B

<

&null;18

AKC 171

25

N2 &null;2

2606.A1

Dead

L2

Dead(L4)/&null;A

L2/L3

<

L2/L3

&null;433

AKC 128

26

N2 &null;31

3313:A10

Dead

Dead

Dead

L1

&null;AD/&null;B

OK

&null;506

AKC 129

27

N2 &null;37

3315:A10

Dead

Dead

L1/L2

L1/L2

OK

L1/L2

&null;484

AKC 130

28

N2 &null;35

3314:D10

Dead

&null;AD/-B

&null;AD/B

&null;AD/&null;B

OK

&null;AD/B&null;

&null;486

AKC 131

29

N2 &null;35

3314:F10

Dead

L1

&null;AD/&null;B

&null;AD/&null;B

&null;AD/&null;B

<

&null;568

AKC 132

30

N2 &null;41

3323:G4

Dead(L2)

Dead

Dead

&null;AD/B

&null;AD/B

Dead

&null;569

AKC 133

31

N2 &null;41

3323:H4

Dead

Dead

Dead

Dead

Dead

Dead

&null;187

AKC 340

32

N2 &null;14

2665:B5

L1

L1

&null;AD/B&null;

&null;AD/B&null;

&null;AD/B

B

&null;133

AKC 134

33

N2 &null;10

2640:A11

Dead

Dead

Dead

&null;AD/B

&null;AD/B

OK

&null;149

AKC 135

34

N2 &null;11

2641:A8

Dead

L1

L1/L2

&null;AD/B

&null;B

&null;AD/B&null;

CONTROL

OK

OK

OK

OK

OK

OK

EXAMPLE 3

Nematicidal Activity of Anthelmintic Compositions 32-46

&null;0399&null; The C. elegans nematode activity assay for anthelmintic compounds 32-46 was similar to that described in Example 2 above, except for the following noted differences. The compound concentrations were adjusted to 140 &null;M and subjected to 2-fold dilutions to yield 140 &null;M, 70 &null;M, 35 &null;M, 17.5 &null;M, 8.8 &null;M, 4.4 &null;M, 2.2 &null;M, and 1.09 &null;M. The visual evaluation of viability was conducted at Day 4, and the results are presented in Table 2.

10

TABLE 2

&null;M Concentration

Compound

140

70

35

17.5

8.8

4.4

2.2

1.09

AKC-138

L1

L1

L1

L2

B

OK

OK

OK

AKC-144

L3/L4

L4/AD/B

B

&null;B

OK

OK

OK

OK

AKC-141

L1

L1

L1

<

OK

OK

OK

OK

AKC-116

L1/L2

L2/L3

L3

B&null;

B

OK

OK

OK

AKC-117

L1/L2

L2/L3

L3

B&null;

B

<

OK

OK

AKC-118

L2

L2/L3

L3

L4/AD/B&null;

B

&null;B

OK

OK

Control

OK

OK

OK

OK

OK

OK

OK

OK

EXAMPLE 4

Activity Against Nematode (C. elegans) Eggs

&null;0400&null; Compositions of the subject invention are surprisingly found to be ovicidal. The following procedures are used to test for lethal effects against nematode eggs.

Materials

&null;0401&null; As referred to herein, &null;S Medium&null; refers to &null;S basal&null; supplemented with CaCl2, MgSO4, and a trace metals solution as follow:

11

S basal

NaCl

5.857 g

1M potassium phosphate (pH 6)

50.0 ml

Cholesterol (5 mg/ml in EtOH)

1.0 ml

dH2O

1 L

&null;0402&null; The above preparation is then autoclaved. S basal can be stored until needed.

&null;0403&null; Just prior to use, S Medium is made from S basal by adding, asceptically, the following components to 1L S basal (components should first be autoclaved separately):

12

1M potassium citrate (pH 6)

10 ml

Trace metals solution (see below)

10 ml

1M CaCl2

3 ml

1M MgSO4

3 ml

Trace Metals solution

Na2EDTA

1.86 g

(to 5 mM)

Fe2SO4.7H2O

0.69 g

(to 2.5 mM)

MnCl2.4H2O

0.20 g

(to 1 mM)

ZnSO4.7H2O

0.29 g

(to 1 mM)

CuSO4.5H2O

0.025 g

(to 0.1 mM)

dH2O

1 L

&null;0404&null; Procedure:

&null;0405&null; 1. Make anthelmintic compound dilutions as indicated in Examples 2-3.

&null;0406&null; 2. To 500 &null;l of each dilution, added 10 &null;l of eggs (estimated >200 eggs/10 &null;l).

&null;0407&null; 3. Mixed well and allowed to incubate at room temperature for from 30 minutes to 3 hours.

&null;0408&null; 4. Centrifuge at 2000 rpm for 5 minutes at room temperature.

&null;0409&null; 5. Pipette off supernatant.

&null;0410&null; 6. Re-suspend in 500 &null;l S Medium.

&null;0411&null; 7. Centrifuge at 2000 rpm for 5 minutes at room temperature

&null;0412&null; 8. Pipette off supernatant.

&null;0413&null; 9. Re-suspend in 300 &null;l S Medium.

&null;0414&null; 10. Transfer 300 &null;l into 24-well tissue culture bioassay tray.

&null;0415&null; 11. Add 2 &null;l of stationary phase E. coli to each well.

&null;0416&null; 12. Score after 3 days at room temperature in the dark.

EXAMPLE 5

Additional Observations of Activity Against Nematode (C. elegans) Eggs

&null;0417&null; Additional tests are conducted to confirm the ovicidal activity. The following procedures are used.

&null;0418&null; 1. Make anthelmintic compound dilutions to 2&null; concentrations shown in Example 4.

&null;0419&null; 2. Distribute 0.5 ml of each dilution into 1.5-ml Eppendorf tubes.

&null;0420&null; 3. Add 0.5 ml of C. elegans egg preparation to 0.5 ml 2&null; dilution to yield final exposure concentration.

&null;0421&null; 4. Mix well and allow to incubate at room temperature for from 30 minutes to 3 hours.

&null;0422&null; 5. Centrifuge at 2000 rpm for 5 minutes at room temperature.

&null;0423&null; 6. Pipette off supernatant and re-suspend in 1.5 ml S Medium.

&null;0424&null; 7. Spin as above for 2 minutes.

&null;0425&null; 8. Pipette off supernatant and re-suspend in 1.5 ml S Medium.

&null;0426&null; 9. Repeat &null;7.

&null;0427&null; 10. Pipette off supernatant and re-suspend in 1.0 ml S Medium.

&null;0428&null; 11. Add 280 &null;l of S Medium to each well of 24-well tissue culture plate.

&null;0429&null; 12. Add 20 &null;l of each treated (and control) sample in triplicate into the respective wells.

&null;0430&null; 13. Score after 3 days at room temperature in the dark.

EXAMPLE 6

Preparation of Anthelmintic Compounds 47, as specifically exemplified by Compounds 48-168

&null;0431&null; While the anthelmintic compounds of the subject invention can readily be produced using procedures well known to those skilled in the art, the following is a preferred method of producing anthelmintic Compounds 47, and exemplified Compounds 48-168, as shown in FIGS. 47-168. The general library scheme resulting in Compounds 47 is depicted in FIG. 169.

&null;0432&null; The addition of secondary amines to 3,5-dichloro-1,2,4-thiadiazole was done on a 10 to 20 mmol scale in standard glassware. Most secondary amine reactions were complete in less than an hour at room temperature. Because of this high reactivity, 3,5-dichloro-1,2,4-thiadiazole was handled as if it was a hazardous material. Thirty-two different 3-chloro-5-alkylamino-1,2,4-thiadiazoles were prepared.

&null;0433&null; Displacement of the second chloro was performed by hand on 0.1 mmoles in septa capped 2 mL vials. Each of 48 amines was distributed into vials and treated with each of 32 mono-chlorothiadiazoles. The vials were heated to 125&null; C. in a vented oven. Each reaction mixture was extracted in a solid supported liquid-liquid format with 2 N hydrochloric acid in 96 well filter plates using Varian Chem Elut hydromatrix.

&null;0434&null; Synthesis of 3-chloro-5-(propylcyclopropanemethylamino)-1,2,4,-thiadiazole. To a solution of 3,5-dichloro-1,2,4-thiadiazole (2.325 g, 15 mmol) in 8 mL of dichloroethane was added dropwise a solution of propylcyclopropanemethylamine (2.10 g, 15.75 mmol) in 12 mL of dichloroethane, followed by dropwise addition of a solution of diisopropylethylamine (2.133 g, 16.5 mmol). The reaction mixture was shaken at room temperature for 24 h. and poured onto an extraction column cartridge (60 mL) which was packed with Varian Chem Elut hydromatrix (15 g) pretreated with 15 mL of water. The column was then washed two times with 50 mL of dichloromethane. The collected extracts were concentrated and dried under high vacuum to yield 3.46 g (100%).

&null;0435&null; Isopropanol was used as the reaction solvent, for amines which are not soluble at these concentrations in dichloroethane. The isopropanol is removed by rotovap before the extraction column which is eluted with chloroform.

&null;0436&null; Preparation of Library: 3,5-dialkylamino-1,2,4-thiadiazole

&null;0437&null; A set of 48 amine solutions (1.0 M) in MeOH/CH2Cl2 (&null;) and a set of thirty-two 5-alkylamino-3-chloro-1,2,4-thiadiazole solutions (2.0 M) in DMSO were prepared. For those 5-alkylamino-3-chloro-1,2,4-thiadiazoles that are not soluble in DMSO, a solution (1.0 M) in MeOH/CH2Cl2 (&null;) was prepared. To each of 1536 septa capped 2 mL autosampler vials placed in Gilson code 209 racks were added 0.05 mL of 5-alkylamino-3-chloro-1,2,4-thiadiazole solution(0.1 mmol, 1.0 eq) and 0.5 mL of amine solution (0.5 mmol, 5.0 eq) by Eppendorf repeater pipette using the following procedure: amine 1 was dispensed into vials 1 to 16, amine 2 is dispensed into vials 17 to 32, and so on. Those 5-alkylamino-3-chloro-1,2,4-thiadiazolesthat are not soluble in DMSO are added at this time. Then the solvents were evaporated in a high vacuum oven at 40 &null; C. for 2 h. Amines which are low boiling liquids evaporate under these conditions. Consequently volatile amines (ACD&null;5949, 5996, 6005, 11690, 134208, 47920)were added to the reaction vials after MeOH/CH2Cl2 evaporation. The 5-alkylamine-3-chloro-1,2,4-thiadiazole solution(0.05 mL, 0.1 mmol, 1.0 eq) in DMSO was then added to each vial by Eppendorf repeater pipette. The first 5-alkylamine-3-chloro-1,2,4-thiadiazole was dispensed to the first vial of each set of 16 vials, the second 5-alkylamine-3-chloro-1,2,4-thiadiazoldis dispensed to the second vial of each set of 16 vials, and so on. For the vials which already contain 5-alkylamino-3-chloro-1,2,4-thiadiazoles, 0.05 mL of DMSO was added. The Gilson racks were placed in an oven and heated at 125&null; C. for 48 h. The amines 37 to 48 are heated at 125&null; C. for 96 h. The fastest reaction and thus the shorter reaction times were for cyclic secondary amines with a structure related to piperidine. Excess amine and much of the DMSO is removed by solid supported liquid-liquid extraction using Varian Chem Elut hydromatrix in Beckman square well plates. The 96 well filter plates (88 format) were drilled and fitted with polyethylene frits and packed with about 2 g of hydromatrix treated with 300 &null;L of 2 N HCl. The fritted plate was placed on top of a second plate for collection of the eluted products. The contents of each reaction vial was dissolved in 200 &null;L chloroform and poured on to the liquid-liquid extraction matrix. The vials were rinsed with 500 &null;L of chloroform. Chloroform (2 mL) was added in 2 portions to elute the products. The collected extracts in an 88 format microtiter plate were concentrated.

EXAMPLE 7

Nematicidal Activity of Anthelmintic Compositions 48-168

&null;0438&null; The nematicidal activity of anthelmintic Compositions 48-168 were determined in accordance with the procedure outlined in Example 2. The results are reported in Table 3.

13

TABLE 3

HTS Data

HTS Tracking

Initial HTS Run

Follow-up HTS Run

5 Day Visual Score

AKC&null;

MP &null;

Well

mOD

% Run

Visual Score

mOD

% Run

Visual Score

Well Addr

V1

V2

V3

V4

V5

V6

218

2628

B3

100

91%

1AD/B&null;

125

78%

Dead

2628:B3

Dead

Dead

&null;AD/B

OK

OK

OK

219

2628

D3

99

90%

4AD/B

119

74%

Dead/1AD/B&null;

2628:D3

Dead

&null;AD/B

&null;AD/B

OK

OK

OK

220

2628

H3

105

95%

1AD/B&null;

121

75%

Dead

2628:H3

Dead

&null;AD/B

&null;B

OK

OK

OK

221

2628

G4

107

97%

L4/AD/B

136

84%

5AD/B

2628:G4

&null;AD/&null;B

L3/L4

OK

OK

OK

OK

222

2628

H6

117

106%

L2-Dead L3&null;

203

126%

L2/L3

2628:H6

&null;AD/&null;B

OK

OK

OK

OK

OK

223

2629

E2

118

107%

L3/1L4

190

118%

Dead

2629:E2

Dead

B

<

OK

OK

OK

B&null;

B&null;

B&null;

OK

OK

OK

224

2630

C4

157

143%

L1/L2

196

122%

L1

2630:C4

Dead

Dead

<

OK

OK

OK

225

2630

C8

125

114%

L2/L3

172

107%

L3/L4

2630:C8

L1/L2

L3/L4

&null;B

OK

OK

OK

226

2630

C10

118

107%

L2/L3

167

104%

L3/L4

2630:C10

L1

&null;B

OK

OK

OK

OK

227

2632

C7

118

107%

Dead

146

91%

Dead

2632:C7

Dead

Dead

OK

OK

OK

OK

228

2632

D7

118

107%

1AD/B&null;

118

73%

Dead/1L4/1AD

2632:D7

Dead

Dead

OK

OK

OK

OK

229

2632

E7

137

125%

Dead

130

81%

5AD/B

2632:E7

Dead

&null;AD/B

&null;AD/&null;B

OK

OK

OK

230

2632

F7

133

121%

Dead/1AD/B&null;

101

63%

3AD/B

2632:F7

Dead

&null;AD/B&null;

&null;AD/B

<

OK

OK

L1

L1

L1

L1/L2

OK

OK

231

2632

B8

131

119%

2AD/B

105

65%

2AD/B

2632:B8

Dead

Dead

<

OK

OK

OK

232

2632

C8

132

120%

2AD/B

109

68%

Dead/1AD/B

2632:C8

Dead

&null;AD/B

OK

OK

OK

OK

233

2632

D8

124

113%

1AD/B

150

93%

Dead

2632:D8

Dead

&null;AD/B

&null;B

OK

OK

OK

234

2632

E8

132

120%

3AD/B

102

63%

2AD/B

2632:E8

Dead

&null;AD/B&null;

&null;B

<

OK

OK

235

2632

G9

141

128%

Dead (L3&null;)

205

127%

L2 - 1/2 Dead

2632:G9

Dead

&null;AD/&null;B

OK

OK

OK

OK

236

2633

G6

150

136%

1AD/B

198

123%

Dead

2633:G6

Dead

L1/L2

L1/L2

<

<

OK

237

2633

A7

146

133%

L1/L2

189

117%

L1/L2

2633:A7

L1

L1

L1

<

OK

OK

B&null;

B&null;

B&null;

OK

OK

OK

238

2633

D7

148

135%

L4/Ad/B&null;

197

122%

L4/AD/B

2633:D7

L1

L2/L3

<

<

OK

OK

239

2633

H8

147

134%

L4/AD/B&null;

212

132%

B

2633:H8

L2

B

OK

OK

OK

OK

240

2634

A11

111

101%

L1/L2

138

86%

Dead

2634:A11

Dead

L1

&null;AD/B

&null;B

<

OK

241

2635

A1

125

114%

L3/L4

141

88%

B

2635:A1

L1/L2

&null;B

OK

OK

OK

OK

242

2635

D1

117

106%

Dead (L3&null;)

140

87%

L1/L2

2635:D1

L1/L2

B

OK

OK

OK

OK

243

2635

E1

127

115%

L4/AD/B&null;

139

86%

B

2635:E1

&null;AD/B&null;

B

OK

OK

OK

OK

244

2635

A4

138

125%

Dead

167

104%

Dead

2635:A4

Dead

Dead

<

OK

OK

OK

L1

L1

L1

L1

L1/L2

<

245

2638

D2

113

103%

B&null;-Few Eggs

189

117%

B&null;

2638:D2

L2/Dead(L3)

&null;AD/&null;B

<

OK

OK

OK

246

2638

F2

135

123%

L3

209

130%

L3/L4

2638:F2

Dead

&null;AD/B

OK

OK

OK

OK

247

2638

A4

100

91%

L2/L3

169

105%

L2/L3

2638:A4

L1/L2

&null;B

OK

OK

OK

OK

248

2638

D4

109

99%

L3/L4/AD/B

181

112%

L3/L4/AD/B&null;

2638:D4

L1/L2

&null;B

<

OK

OK

OK

249

2638

E4

108

98%

L2/L3

180

112%

L2/L3

2638:E4

L2/L4

&null;B

OK

OK

OK

OK

250

2638

F4

119

108%

L2 - (Dead L3)

201

125%

L2/L3

2638:F4

L3/Dead(L3)

L4/&null;AD/B&null;

B

OK

OK

OK

251

2638

G4

101

92%

L4/AD/B&null;-Few Eggs

178

111%

L4/AD/B&null;

2638:G4

&null;AD/B&null;

B

OK

OK

OK

OK

B&null;

B&null;

B&null;

OK

OK

OK

252

2638

A5

110

100%

Dead(L3)

161

100%

L2 - Dead L3

2638:A5

Dead(L3)

Dead(L3)

&null;AD/B

<

<

<

253

2638

C5

112

102%

L2/L3

181

112%

L2/L3

2638:C5

Dead

L2/L3

&null;B

OK

OK

OK

254

2638

D5

116

105%

Dead(L3)

182

113%

L2 - Dead L3

2638:D5

Dead(L3)

&null;AD/B

<

OK

OK

OK

255

2638

E5

103

94%

Dead(L3)

157

98%

L1/L2 - Dead L3

2638:E5

Dead

Dead

OK

OK

OK

OK

256

2638

F5

117

106%

L2 - (Dead L3)

180

112%

L2 - Dead L3

2638:F5

Dead

&null;AD/B

OK

OK

OK

OK

257

2638

G5

100

91%

L4/AD/B&null;

168

104%

L4/AD/B&null;

2638:G5

L1

&null;B

OK

OK

OK

OK

258

2638

F6

122

111%

L4/AD/B&null;-Few Eggs

200

124%

B&null;

2638:F6

&null;AD/B&null;

&null;AD/B

&null;B

&null;B

OK

OK

L1

L1

L1

L1/L2

&null;AD/B

<

259

2638

G6

119

108%

L4/AD/B&null;-Clear, Few Eg

212

132%

B&null;

2638:G6

L3/L4

B

<

<

<

<

260

2638

H7

134

122%

L2 (Dead L3)

236

147%

L2

2638:H7

L1

B

OK

OK

OK

OK

261

2638

A10

106

96%

L3/L4/AD/B&null;

155

96%

L2/L3

2638:A10

Dead

&null;AD/B

OK

OK

OK

OK

262

2638

B10

121

110%

L3/L4/AD/B&null;

171

106%

L2/L3

2638:B10

L4/&null;AD/B&null;

<

OK

OK

OK

OK

263

2638

C10

121

110%

L3/L4

167

104%

L2/L3

2638:C10

&null;AD/B

&null;B

OK

OK

OK

OK

264

2638

D10

114

104%

L4/AD/B

177

110%

L4/AD/B

2638:D10

L2

&null;AD/B

<

OK

OK

OK

265

2638

E10

119

108%

L2/L3/L4

178

111%

L2/L3/L4

2638:E10

L3/L4

OK

OK

OK

OK

OK

B&null;

B&null;

B&null;

OK

OK

OK

266

2638

F10

121

110%

L2/L3

200

124%

L2/L3/L4

2638:F10

Dead

&null;AD/B&null;

&null;AD/B

OK

OK

OK

267

2639

A6

103

94%

Dead

160

99%

Dead

2639:A6

Dead

L1

<

OK

OK

OK

268

2639

A8

141

128%

Dead

154

96%

Dead

2639:A8

Dead

Dead

&null;AD/B

L4&null;AD/B

OK

B

269

2639

C8

183

166%

L4/1AD/B

212

132%

L4/AD/B

2639:C8

Dead

&null;AD/B

OK

OK

OK

OK

270

2639

E8

164

149%

L1/L2

182

113%

L1/L2

2639:E8

Dead

&null;AD/B

&null;AD/B

OK

OK

OK

271

2639

A10

111

101%

L4/AD/B

175

109%

L3/L4

2639:A10

Dead

&null;AD/B

&null;AD/B

<

OK

<

272

2640

C4

119

108%

L3

177

110%

L2/L3/L4

2640:C4

Dead

B

OK

OK

OK

OK

Dead

DeadDead

Dead

L1

OK

OK

273

2640

A5

111

101%

L1/L2

151

94%

Dead

2640:A5

Dead

Dead

B

&null;B

OK

OK

274

2640

B5

122

111%

Dead

187

116%

Dead

2640:B5

Dead

Dead

&null;AD/B&null;

B

OK

OK

275

2640

C5

105

95%

Dead

169

105%

Dead

2640:C5

Dead

Dead

OK

OK

OK

OK

276

2640

D5

128

116%

Dead

187

116%

Dead

2640:D5

Dead

Dead

L3/L4

OK

OK

OK

277

2640

E5

129

117%

Dead

174

108%

Dead

2640:E5

Dead

Dead

&null;AD/B

OK

OK

OK